Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   NTHIC486_RS03455 Genome accession   NZ_CP007471
Coordinates   678452..679144 (+) Length   230 a.a.
NCBI ID   WP_005686720.1    Uniprot ID   -
Organism   Haemophilus influenzae strain C486     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673452..684144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHIC486_RS03435 (NTHIC486_00687) ampD 674723..675283 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NTHIC486_RS03440 (NTHIC486_00688) pilA 675398..675847 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NTHIC486_RS03445 (NTHIC486_00689) pilB 675844..677241 (+) 1398 WP_005686724.1 GspE/PulE family protein Machinery gene
  NTHIC486_RS03450 (NTHIC486_00690) pilC 677238..678455 (+) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  NTHIC486_RS03455 (NTHIC486_00691) pilD 678452..679144 (+) 693 WP_005686720.1 prepilin peptidase Machinery gene
  NTHIC486_RS03460 (NTHIC486_00692) rho 679198..680460 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  NTHIC486_RS03465 (NTHIC486_00693) metJ 680708..681025 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  NTHIC486_RS03470 (NTHIC486_00694) cueR 681039..681425 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  NTHIC486_RS03475 (NTHIC486_00695) - 681502..681708 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03480 (NTHIC486_00696) - 681794..682000 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03485 (NTHIC486_00697) - 682086..682292 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  NTHIC486_RS03490 (NTHIC486_00698) - 682378..682584 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26677.71 Da        Isoelectric Point: 8.8394

>NTDB_id=119747 NTHIC486_RS03455 WP_005686720.1 678452..679144(+) (pilD) [Haemophilus influenzae strain C486]
MIYFTMFLLGGIFGIALWFYLSGFITRLQQEIYATYIELFPQNFLPFQPSVSSIQQKKCGHILRYFLNVGVGFIFLQIAF
KDSIFAIWLGLTLLILWAISYLDWYYQLISTTPCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=119747 NTHIC486_RS03455 WP_005686720.1 678452..679144(+) (pilD) [Haemophilus influenzae strain C486]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTCGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTCCCACAAAACTTTCTGCCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGGATTTATATTTTTACAAATTGCTTTC
AAGGACTCTATTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATTAGTTATCTTGATTGGTACTATCA
ACTTATTTCTACTACACCCTGTTTATGGCTACTTACAATCGGTTTATTTGGGGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGTATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACTTTGCCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

90.435

100

0.904
  pilD Haemophilus influenzae Rd KW20

90

100

0.9

Multiple sequence alignment