Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU32_RS18295 Genome accession   NZ_CP155982
Coordinates   4233651..4234784 (+) Length   377 a.a.
NCBI ID   WP_012439300.1    Uniprot ID   Q93S76
Organism   Xanthomonas campestris pv. campestris strain CFBP 1121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4228651..4239784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU32_RS18270 (ABFU32_18260) - 4228827..4230026 (-) 1200 WP_068573752.1 cystathionine gamma-synthase -
  ABFU32_RS18275 (ABFU32_18265) - 4230120..4231490 (-) 1371 WP_011035841.1 pyridoxal-phosphate dependent enzyme -
  ABFU32_RS18280 (ABFU32_18270) - 4231722..4231940 (-) 219 WP_019237985.1 YdcH family protein -
  ABFU32_RS18285 (ABFU32_18275) - 4232282..4233172 (-) 891 WP_014506548.1 membrane protein -
  ABFU32_RS18290 (ABFU32_18280) - 4233169..4233552 (-) 384 WP_068573754.1 DUF4398 domain-containing protein -
  ABFU32_RS18295 (ABFU32_18285) pilU 4233651..4234784 (+) 1134 WP_012439300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU32_RS18300 (ABFU32_18290) maiA 4235306..4235968 (-) 663 WP_068573756.1 maleylacetoacetate isomerase -
  ABFU32_RS18305 (ABFU32_18295) - 4236012..4237001 (-) 990 WP_068573758.1 fumarylacetoacetate hydrolase family protein -
  ABFU32_RS18310 (ABFU32_18300) - 4237381..4238769 (+) 1389 WP_011035834.1 DEAD/DEAH box helicase -
  ABFU32_RS18315 (ABFU32_18305) queD 4238885..4239238 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ABFU32_RS18320 (ABFU32_18310) - 4239428..4239763 (+) 336 WP_068573760.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41799.10 Da        Isoelectric Point: 6.1401

>NTDB_id=1000698 ABFU32_RS18295 WP_012439300.1 4233651..4234784(+) (pilU) [Xanthomonas campestris pv. campestris strain CFBP 1121]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1000698 ABFU32_RS18295 WP_012439300.1 4233651..4234784(+) (pilU) [Xanthomonas campestris pv. campestris strain CFBP 1121]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACGCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGTTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAAAGTGCGCACAAGAACGTGCTGATGAACCTGGCACTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGACTGGACGGGCGCCGGCGCCCTGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGTCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGATCCGTACGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93S76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363

Multiple sequence alignment