Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS17507  |
| T6SS type | Type i4a |
| Strain | Cupriavidus pinatubonensis JMP134 |
| Replicon (RefSeq) | chromosome I (NC_007347) |
| Location | 1848935..1873477 |
| Function | anti-bacterial; anti-adverse environment |
| Comment | The Fur-regulated T6SS1 plays an important role in iron acquisition in C. necator and TeoL-mediated OMV recruitment is crucial for exploitation competition, oxidative stress resistance, and horizontal gene transfer |
| References | [1] Li C, Zhu L, Wang D, Wei Z, Hao X, Wang Z, Li T, Zhang L, Lu Z, Long M, Wang Y, Wei G, Shen X. et al (2021) T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer.. ISME J. NA. [PudMed:34433898] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| REUT_RS08605 | 1844616..1845830 | - | 1215 | WP_011297872.1 | MFS transporter | ||
| REUT_RS08610 | 1845827..1846192 | - | 366 | WP_011297873.1 | RidA family protein | ||
| REUT_RS08615 | 1846305..1847138 | + | 834 | WP_011297874.1 | TrmB family transcriptional regulator | ||
| REUT_RS08620 | 1847393..1847650 | - | 258 | pseudogene | helix-turn-helix domain-containing protein | ||
| REUT_RS08625 | 1847968..1848882 | - | 915 | WP_011297876.1 | hypothetical protein | ||
| T6CP259588 | TssA | REUT_RS08630 (tssA) | 1848935..1850080 | - | 1146 | WP_049796458.1 | type VI secretion system protein TssA |
| T6CP259589 | TssM | REUT_RS08635 | 1850094..1854242 | - | 4149 | WP_011297878.1 | type VI secretion system protein |
| T6CP259590 | TssL | REUT_RS08640 | 1854280..1855032 | - | 753 | WP_011297879.1 | DotU family type IV/VI secretion system protein |
| T6CP259591 | TssK | REUT_RS08645 (tssK) | 1855058..1856386 | - | 1329 | WP_011297880.1 | type VI secretion system baseplate subunit TssK |
| T6CP259592 | TssJ | REUT_RS08650 | 1856453..1856965 | - | 513 | WP_011297881.1 | hypothetical protein |
| REUT_RS08655 | 1856997..1858430 | - | 1434 | WP_011297882.1 | cation:dicarboxylase symporter family transporter | ||
| REUT_RS08660 | 1858427..1859128 | - | 702 | WP_011297883.1 | amino acid racemase | ||
| REUT_RS08665 | 1859151..1860425 | - | 1275 | WP_011297884.1 | cation:dicarboxylase symporter family transporter | ||
| REUT_RS08670 | 1860446..1861465 | - | 1020 | WP_086004423.1 | ABC transporter substrate-binding protein | ||
| REUT_RS08675 | 1861486..1861746 | - | 261 | WP_011297886.1 | hypothetical protein | ||
| REUT_RS08680 | 1861754..1862140 | - | 387 | WP_011297887.1 | DUF4280 domain-containing protein | ||
| REUT_RS08685 | 1862145..1862768 | - | 624 | WP_140950547.1 | hypothetical protein | ||
| T6CP259594 | TssI | REUT_RS08690 (tssI) | 1862870..1864798 | - | 1929 | WP_011297889.1 | type VI secretion system tip protein VgrG |
| T6CP259595 | TssH | REUT_RS08695 (tssH) | 1864803..1867499 | - | 2697 | WP_011297890.1 | type VI secretion system ATPase TssH |
| T6CP259596 | TssG | REUT_RS08700 (tssG) | 1867512..1868585 | - | 1074 | WP_041679606.1 | type VI secretion system baseplate subunit TssG |
| T6CP259597 | TssF | REUT_RS08705 (tssF) | 1868540..1870291 | - | 1752 | WP_086004442.1 | type VI secretion system baseplate subunit TssF |
| T6CP259598 | TssE | REUT_RS08710 (tssE) | 1870293..1870757 | - | 465 | WP_011297893.1 | type VI secretion system baseplate subunit TssE |
| T6CP259599 | TssD | REUT_RS08715 | 1870799..1871281 | - | 483 | WP_011297894.1 | type VI secretion system tube protein Hcp |
| T6CP259600 | TssC | REUT_RS08720 (tssC) | 1871383..1872885 | - | 1503 | WP_011297895.1 | type VI secretion system contractile sheath large subunit |
| T6CP259601 | TssB | REUT_RS08725 (tssB) | 1872929..1873477 | - | 549 | WP_011297896.1 | type VI secretion system contractile sheath small subunit |
| REUT_RS08730 | 1874046..1874753 | + | 708 | WP_041679607.1 | response regulator transcription factor | ||
| REUT_RS08735 | 1874921..1876354 | - | 1434 | WP_011297898.1 | HDOD domain-containing protein | ||
| REUT_RS08740 | 1876363..1878144 | - | 1782 | WP_140950546.1 | GGDEF domain-containing protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0379 |
REUT_RS14415 (fur) | protein | Global regulator | Cupriavidus pinatubonensis JMP134 | chromosome (NC_007347) | 3110053..3110484 (+) | WP_011298983.1 | reut_A1733 promotor (↑) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF76648 |
REUT_RS08685 (teoL) | - | - | Cupriavidus pinatubonensis JMP134 | chromosome (NC_007347) | 1862145..1862768 (-) | WP_140950547.1 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|---|---|---|---|---|---|---|---|
| ACP44068 |
REUT_RS18795 (cubA) | chaperone | Cupriavidus pinatubonensis JMP134 | chromosome (NC_007348) | 269098..271227 (-) | WP_041680124.1 | - | REUT_RS08685 (teoL) |
| ACP44069 |
REUT_RS23710 (cstR) | chaperone | Cupriavidus pinatubonensis JMP134 | chromosome (NC_007348) | 1349588..1351858 (+) | WP_041680249.1 | - | REUT_RS08685 (teoL) |