Detailed information of component protein
Summary
| Component ID | T6CP259590 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS17507  (Type i4a) |
| Strain | Cupriavidus pinatubonensis JMP134 |
| Replicon | chromosome I |
| Sequence | Protein sequence (251 a.a.); Nucleotide sequence (753 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | REUT_RS08640 |
| Coordinate (Strand) | 1854280..1855032 (-) |
| NCBI ID | WP_011297879.1 |
| RefSeq | NC_007347 |
| Uniprot ID | Q470V2_CUPPJ |
| KEGG ID | reu:Reut_A1716 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 1.8e-34 | 20..224 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-209 | MARYRPEAEDDDLAGEQFRAFLDELVNAQARLSESADATPEAVAQGMSRHLLNLLELQTLQSRRDSTRFEMEDVADARYLKAVLADEILLNTDWAGQAQWHAHLLESTLFRTNIAGDEVFRRIEQLLLDREPSRRKLARLYLSALALGFQGRFRGADADARLRSLREELYEFVYQRRPDLSGRDRVVSEAAYAHTLSHIAPRKLPTMTR |
| Transmembrane helix | 210-232 | WTVVFALSLVSLLALSEVLWLWQ |
| Outside | 233-250 | SWPLRQALRADTVVGANP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9903 | - | - |
Protein sequence: 251 a.a.
>T6CP259590 NC_007347:c1855032-1854280 [Cupriavidus pinatubonensis JMP134] [TssL]
MARYRPEAEDDDLAGEQFRAFLDELVNAQARLSESADATPEAVAQGMSRHLLNLLELQTLQSRRDSTRFEMEDVADARYL
KAVLADEILLNTDWAGQAQWHAHLLESTLFRTNIAGDEVFRRIEQLLLDREPSRRKLARLYLSALALGFQGRFRGADADA
RLRSLREELYEFVYQRRPDLSGRDRVVSEAAYAHTLSHIAPRKLPTMTRWTVVFALSLVSLLALSEVLWLWQSWPLRQAL
RADTVVGANP*
MARYRPEAEDDDLAGEQFRAFLDELVNAQARLSESADATPEAVAQGMSRHLLNLLELQTLQSRRDSTRFEMEDVADARYL
KAVLADEILLNTDWAGQAQWHAHLLESTLFRTNIAGDEVFRRIEQLLLDREPSRRKLARLYLSALALGFQGRFRGADADA
RLRSLREELYEFVYQRRPDLSGRDRVVSEAAYAHTLSHIAPRKLPTMTRWTVVFALSLVSLLALSEVLWLWQSWPLRQAL
RADTVVGANP*
Nucleotide sequence: 753 bp
>T6CP259590 NC_007347:c1855032-1854280 [Cupriavidus pinatubonensis JMP134] [TssL]
ATGGCACGCTATCGACCCGAAGCCGAGGACGACGATCTCGCCGGCGAGCAGTTCCGCGCCTTCCTGGACGAACTGGTCAA
TGCCCAGGCGCGCCTGTCCGAGAGTGCCGACGCCACGCCCGAGGCGGTGGCGCAGGGCATGAGCCGCCACCTGCTGAACC
TGCTGGAGTTGCAGACTTTGCAGTCGCGCCGCGACAGCACGCGCTTCGAGATGGAGGACGTCGCCGACGCCCGCTACCTG
AAGGCGGTGCTCGCCGACGAGATCCTGCTCAACACCGATTGGGCGGGCCAGGCGCAGTGGCATGCCCACCTGCTGGAATC
GACGCTGTTCCGCACCAATATCGCCGGCGATGAGGTGTTCCGCCGCATTGAGCAGTTGCTGCTGGATCGCGAGCCATCGC
GCCGAAAGCTGGCCCGGCTGTACTTGTCTGCGCTGGCGCTGGGCTTTCAGGGGCGCTTTCGCGGCGCTGATGCCGACGCA
CGCCTGCGCAGTCTGCGCGAGGAGTTGTATGAGTTTGTCTACCAGCGCCGTCCCGACTTGAGCGGGCGCGACCGCGTGGT
CAGCGAGGCCGCGTACGCGCACACGCTATCCCATATCGCGCCGCGCAAGCTGCCTACCATGACGCGCTGGACCGTGGTGT
TCGCGCTCAGCCTGGTCAGCCTGCTGGCCCTGTCCGAGGTGCTGTGGCTGTGGCAGTCGTGGCCGCTGCGCCAGGCATTG
CGCGCCGATACGGTGGTGGGAGCCAATCCATGA
ATGGCACGCTATCGACCCGAAGCCGAGGACGACGATCTCGCCGGCGAGCAGTTCCGCGCCTTCCTGGACGAACTGGTCAA
TGCCCAGGCGCGCCTGTCCGAGAGTGCCGACGCCACGCCCGAGGCGGTGGCGCAGGGCATGAGCCGCCACCTGCTGAACC
TGCTGGAGTTGCAGACTTTGCAGTCGCGCCGCGACAGCACGCGCTTCGAGATGGAGGACGTCGCCGACGCCCGCTACCTG
AAGGCGGTGCTCGCCGACGAGATCCTGCTCAACACCGATTGGGCGGGCCAGGCGCAGTGGCATGCCCACCTGCTGGAATC
GACGCTGTTCCGCACCAATATCGCCGGCGATGAGGTGTTCCGCCGCATTGAGCAGTTGCTGCTGGATCGCGAGCCATCGC
GCCGAAAGCTGGCCCGGCTGTACTTGTCTGCGCTGGCGCTGGGCTTTCAGGGGCGCTTTCGCGGCGCTGATGCCGACGCA
CGCCTGCGCAGTCTGCGCGAGGAGTTGTATGAGTTTGTCTACCAGCGCCGTCCCGACTTGAGCGGGCGCGACCGCGTGGT
CAGCGAGGCCGCGTACGCGCACACGCTATCCCATATCGCGCCGCGCAAGCTGCCTACCATGACGCGCTGGACCGTGGTGT
TCGCGCTCAGCCTGGTCAGCCTGCTGGCCCTGTCCGAGGTGCTGTGGCTGTGGCAGTCGTGGCCGCTGCGCCAGGCATTG
CGCGCCGATACGGTGGTGGGAGCCAATCCATGA