Detailed information of accessory protein


Summary

Accessory protein ID ACP44069 experimental
Type chaperone
Related T6SS (Type) T6SS17507 (Type i4a)
Strain Cupriavidus pinatubonensis JMP134
Replicon chromosome
Related structure protein -
Related effector REUT_RS08685 (teoL)
Description The TeoL effector protein selectively binds to OMVs first, then brings the iron-containing OMVs to the bacterial cell surface by interacting with the CubA/CstR outer membrane receptors.
Reference [1] Li C, Zhu L, Wang D, et al. T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer. ISME J. 2022 Feb;16(2):500-510. doi: 10.1038/s41396-021-01093-8. Epub 2021 Aug 25. PMID: 34433898
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) REUT_RS23710 (cstR)
Coordinates (Strand) 1349588..1351858 (+)
NCBI ID WP_041680249.1
RefSeq NC_007348
Uniprot ID UPI0005A0B0F0
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
TonB_dep_Rec PF00593.26 7e-58 273..755
Plug PF07715.17 5.1e-21 77..174
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-756 VEGKQEKFRLNPLAAALTGTVAAFAMQSAGAQENQPAAELAAASKPVTLQSVTVTSTKDDGYKVDEAQSSKFTAPLLDTPKSVTVVPAEVLQQTGSNSLQDALRTTPGITFGAGEGGNPIGDRPFIRGFDSMSSIYVDGVRDTASQTRDMFNIDRVEVIKGPSSAFGGKGSAGGMINLVSKLPTAENFVQGSVGVGTDSYFRATADGNYLLNDNTAVRLNAMGQSFDVPGRNEVDQHSWGFAPSVTFGLSSPTQVTLSYYHLQGDGMPDYSIPYARPSSQATKANPSAPASVDRNNFYGLTDRDFQRTQSDIATASVQHDFRNGLVFRNTTRWGRATNDYVVTNPDDSRGNVPRGFVWRNTKNRDSATETLTNQTDLTSEFHTGSLKHNLLFGFEFSRDNTDNNPYNVVSANAAASGGTCNASLLASFDCAPLGNPNPNDPWRGSITKMPYTQTTTTNTQSVYLFDTIEITKQWLVNGGIRYDNYDTHSFTNAYTNPNTGAAVAQVDIRNQSHFFNYQAGVVYKPVEYGSIYVSYGTSSTPPGSTNGDGADNITAAQRNLEPETARSFEIGTKWDLLSRRLSLTGAIFQIEKNNARVAIDANTTVNAGKQRVKGFELGFAGNLTDKWGVFGGYTHLDSELIDNGPQAANAANNGNQFPNTPKSSFSLWTTYQVLAPLTIGAGAYYVDKVYANPANSLYVPSYWRFDLMGAYRVNKNLTLQLNIQNLFDKTYYTKAHTHYGALAPGRFGMLTANFRF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.7203 pos: 31-32. AGA-QE. VEGKQEKFRLNPLAAALTGTVAAFAMQSAGA


  Protein sequence: 757 a.a.    

>ACP44069 NC_007348:1349588-1351858 [Cupriavidus pinatubonensis JMP134]
VEGKQEKFRLNPLAAALTGTVAAFAMQSAGAQENQPAAELAAASKPVTLQSVTVTSTKDDGYKVDEAQSSKFTAPLLDTP
KSVTVVPAEVLQQTGSNSLQDALRTTPGITFGAGEGGNPIGDRPFIRGFDSMSSIYVDGVRDTASQTRDMFNIDRVEVIK
GPSSAFGGKGSAGGMINLVSKLPTAENFVQGSVGVGTDSYFRATADGNYLLNDNTAVRLNAMGQSFDVPGRNEVDQHSWG
FAPSVTFGLSSPTQVTLSYYHLQGDGMPDYSIPYARPSSQATKANPSAPASVDRNNFYGLTDRDFQRTQSDIATASVQHD
FRNGLVFRNTTRWGRATNDYVVTNPDDSRGNVPRGFVWRNTKNRDSATETLTNQTDLTSEFHTGSLKHNLLFGFEFSRDN
TDNNPYNVVSANAAASGGTCNASLLASFDCAPLGNPNPNDPWRGSITKMPYTQTTTTNTQSVYLFDTIEITKQWLVNGGI
RYDNYDTHSFTNAYTNPNTGAAVAQVDIRNQSHFFNYQAGVVYKPVEYGSIYVSYGTSSTPPGSTNGDGADNITAAQRNL
EPETARSFEIGTKWDLLSRRLSLTGAIFQIEKNNARVAIDANTTVNAGKQRVKGFELGFAGNLTDKWGVFGGYTHLDSEL
IDNGPQAANAANNGNQFPNTPKSSFSLWTTYQVLAPLTIGAGAYYVDKVYANPANSLYVPSYWRFDLMGAYRVNKNLTLQ
LNIQNLFDKTYYTKAHTHYGALAPGRFGMLTANFRF*

  Nucleotide sequence: 2271 bp    

>ACP44069 NC_007348:1349588-1351858 [Cupriavidus pinatubonensis JMP134]
GTGGAAGGAAAACAGGAAAAATTCCGTTTGAACCCGCTCGCCGCAGCGCTAACCGGTACCGTCGCGGCGTTCGCCATGCA
AAGCGCCGGCGCGCAGGAAAACCAGCCGGCCGCCGAGCTTGCGGCCGCGTCGAAGCCGGTGACGCTGCAAAGCGTGACGG
TGACGAGCACGAAGGACGATGGCTACAAGGTGGACGAAGCCCAGTCGTCCAAGTTCACCGCCCCGCTGCTCGACACGCCG
AAGTCCGTCACCGTCGTGCCGGCCGAAGTGCTGCAGCAGACCGGCTCCAACTCGCTGCAGGACGCCTTGCGTACCACGCC
GGGCATCACGTTCGGCGCCGGCGAAGGCGGCAACCCGATCGGCGACCGTCCTTTCATCCGCGGTTTCGACTCGATGTCGA
GCATCTACGTCGATGGCGTGCGCGACACAGCCTCGCAGACGCGCGACATGTTCAACATCGACCGCGTCGAAGTCATCAAG
GGACCGAGCTCGGCCTTCGGCGGCAAGGGTTCGGCCGGCGGCATGATCAACCTCGTGTCGAAGCTGCCCACCGCCGAGAA
CTTCGTGCAGGGTTCGGTCGGCGTGGGCACGGATTCGTACTTCCGCGCCACGGCTGACGGCAACTACCTTCTCAACGACA
ACACCGCGGTGCGCCTGAACGCGATGGGCCAGTCCTTCGACGTGCCGGGCCGCAACGAAGTCGACCAGCACTCTTGGGGC
TTTGCGCCATCGGTGACCTTCGGCCTGAGTTCGCCCACGCAGGTCACGCTGTCCTACTACCACTTGCAGGGCGACGGCAT
GCCTGACTACAGCATCCCGTACGCGCGCCCGTCCAGCCAGGCGACCAAGGCCAACCCGTCGGCGCCGGCCAGCGTCGACC
GCAATAACTTCTACGGGCTGACCGATCGCGACTTCCAGCGTACGCAGTCCGACATTGCCACCGCATCGGTCCAGCATGAT
TTCCGCAACGGCCTCGTGTTCCGCAACACGACACGCTGGGGCCGTGCAACGAACGACTACGTCGTCACGAATCCCGACGA
CAGCCGCGGCAACGTGCCGCGCGGCTTCGTATGGCGCAACACCAAGAACCGCGACTCGGCCACCGAGACCCTGACCAACC
AGACCGACCTGACGTCGGAGTTCCACACCGGCAGCCTCAAGCACAACCTGCTGTTCGGCTTCGAGTTCAGCCGCGATAAC
ACGGACAACAACCCGTACAACGTGGTGTCGGCCAACGCGGCCGCATCGGGTGGCACTTGCAACGCTTCGCTACTGGCGAG
CTTCGACTGCGCGCCGCTGGGCAATCCGAACCCGAACGATCCGTGGCGCGGCAGCATCACCAAGATGCCGTACACGCAGA
CCACAACGACCAATACGCAATCGGTCTACCTGTTCGACACGATCGAAATCACCAAGCAGTGGCTGGTCAACGGCGGCATC
CGCTACGACAACTACGACACGCACTCCTTCACCAACGCGTACACCAACCCGAACACCGGCGCGGCCGTGGCGCAGGTGGA
CATCCGCAACCAGTCGCACTTCTTCAACTACCAGGCCGGCGTGGTGTACAAGCCGGTCGAGTACGGCAGCATCTATGTGT
CGTACGGCACGTCGTCGACGCCGCCCGGTTCGACCAATGGCGACGGCGCGGACAACATCACCGCGGCGCAGCGCAATCTC
GAGCCTGAAACCGCGCGCTCGTTCGAAATCGGCACCAAGTGGGACCTGCTGTCGCGCCGCCTGTCGCTGACCGGTGCGAT
CTTCCAGATCGAGAAGAACAATGCGCGCGTGGCCATTGACGCCAACACTACCGTCAACGCGGGCAAGCAGCGCGTGAAGG
GCTTCGAGCTGGGCTTCGCCGGCAACCTGACCGACAAGTGGGGCGTATTCGGCGGCTATACCCACCTGGACAGCGAACTG
ATCGATAACGGCCCGCAAGCCGCGAACGCCGCCAACAACGGCAACCAGTTCCCCAACACGCCGAAAAGCAGCTTCAGCCT
GTGGACGACTTACCAGGTACTGGCACCGCTGACGATCGGCGCAGGCGCCTACTATGTCGACAAGGTCTACGCCAATCCGG
CCAACTCGCTGTACGTGCCGTCGTACTGGCGCTTCGACCTGATGGGTGCGTACCGCGTGAACAAGAACCTGACGCTGCAG
TTGAACATCCAGAACCTGTTCGACAAGACGTACTACACCAAGGCGCATACGCACTACGGCGCTCTGGCGCCGGGCCGCTT
TGGTATGCTGACGGCGAACTTCAGGTTCTGA