Detailed information of accessory protein


Summary

Accessory protein ID ACP44068 experimental
Type chaperone
Related T6SS (Type) T6SS17507 (Type i4a)
Strain Cupriavidus pinatubonensis JMP134
Replicon chromosome
Related structure protein -
Related effector REUT_RS08685 (teoL)
Description The TeoL effector protein selectively binds to OMVs first, then brings the iron-containing OMVs to the bacterial cell surface by interacting with the CubA/CstR outer membrane receptors.
Reference [1] Li C, Zhu L, Wang D, et al. T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer. ISME J. 2022 Feb;16(2):500-510. doi: 10.1038/s41396-021-01093-8. Epub 2021 Aug 25. PMID: 34433898
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) REUT_RS18795 (cubA)
Coordinates (Strand) 269098..271227 (-)
NCBI ID WP_041680124.1
RefSeq NC_007348
Uniprot ID UPI000039FBC0
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
TonB_dep_Rec PF00593.26 6.4e-38 283..707
Plug PF07715.17 9.2e-16 68..169
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-709 MAGLLALTTLDPARAQDGATATGNVSAQTLPEVRVTADASPEDLPAPYAGGQVARGARLGVLGNTSIMKAPFNVSSYTAQMIQDQQSPTLADAVNKDPSVRFTGLPGGNIDNFYIRGFPVGEGNSGEIAFDGLYGVAPNYRVFTDYVERLEVIKGPAAMLYGMSPNSGVGGVINIVPKRAHEDLASVTASYASDTQFGGHVDLSQRFGESREWGVRFNGSYYQGDTPMDNQSRKAGIGALSLDYQGKQLRASLDVIGQQEKIDAPGRAFIMGPGLQAIPGAPDGRRNVTQPWEWTRGTDESLLFRTEYDINDHLTVFADAGGGWTQLSRLFGTTPQIVNAQGDTLSTPTYYKFKVDRATYDAGLRSRFDTGPLRHAVTFQASAYADSLDRANTTGTPVASNIYNPVVRPAQGLPAPDSVPKVSDNDQTGFSLADTMSALDDRVLLTLGVRQQRIRSRNFGATGAVTSSYEESATTPMAGLVVQPWKRVSLYANYVQGLSKGDVAPPTASNSGEVFAPYKTTQYEVGTKVDFGRFSATLAAFQISKPSGQLGGNVFSVNGEQRNRGLELNLFGQVAPGVRLLGGVALIDGEITRSGTAAAVGKDPIGVPRVQANLGAEWDLPWVAGLTVSGTMIYTGRQYVNQLNTASLPSWARFDLGARYRTRITGKTTTFRANVLNVFDRNYWSGVTSWGGFSQAAPRTVMLSATVEF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.5425 pos: 15-16. ARA-QD. MAGLLALTTLDPARA


  Protein sequence: 710 a.a.    

>ACP44068 NC_007348:c271227-269098 [Cupriavidus pinatubonensis JMP134]
MAGLLALTTLDPARAQDGATATGNVSAQTLPEVRVTADASPEDLPAPYAGGQVARGARLGVLGNTSIMKAPFNVSSYTAQ
MIQDQQSPTLADAVNKDPSVRFTGLPGGNIDNFYIRGFPVGEGNSGEIAFDGLYGVAPNYRVFTDYVERLEVIKGPAAML
YGMSPNSGVGGVINIVPKRAHEDLASVTASYASDTQFGGHVDLSQRFGESREWGVRFNGSYYQGDTPMDNQSRKAGIGAL
SLDYQGKQLRASLDVIGQQEKIDAPGRAFIMGPGLQAIPGAPDGRRNVTQPWEWTRGTDESLLFRTEYDINDHLTVFADA
GGGWTQLSRLFGTTPQIVNAQGDTLSTPTYYKFKVDRATYDAGLRSRFDTGPLRHAVTFQASAYADSLDRANTTGTPVAS
NIYNPVVRPAQGLPAPDSVPKVSDNDQTGFSLADTMSALDDRVLLTLGVRQQRIRSRNFGATGAVTSSYEESATTPMAGL
VVQPWKRVSLYANYVQGLSKGDVAPPTASNSGEVFAPYKTTQYEVGTKVDFGRFSATLAAFQISKPSGQLGGNVFSVNGE
QRNRGLELNLFGQVAPGVRLLGGVALIDGEITRSGTAAAVGKDPIGVPRVQANLGAEWDLPWVAGLTVSGTMIYTGRQYV
NQLNTASLPSWARFDLGARYRTRITGKTTTFRANVLNVFDRNYWSGVTSWGGFSQAAPRTVMLSATVEF*

  Nucleotide sequence: 2130 bp    

>ACP44068 NC_007348:c271227-269098 [Cupriavidus pinatubonensis JMP134]
ATGGCAGGCCTGCTGGCATTGACCACCCTGGACCCCGCCCGGGCTCAGGACGGCGCCACTGCCACCGGCAATGTCAGTGC
GCAAACCTTGCCGGAGGTCCGTGTCACCGCCGATGCGTCACCGGAAGACCTGCCCGCGCCCTATGCCGGCGGCCAGGTCG
CGCGCGGTGCGCGGCTTGGTGTGCTGGGCAACACCAGCATCATGAAGGCGCCGTTCAACGTGAGCAGCTACACCGCGCAG
ATGATCCAGGACCAGCAATCTCCAACGCTGGCTGACGCTGTCAACAAGGATCCATCGGTGCGATTCACCGGCCTGCCCGG
CGGCAACATCGACAACTTCTATATCCGCGGCTTCCCGGTAGGCGAAGGCAACAGCGGCGAAATCGCATTCGACGGCCTGT
ACGGCGTGGCACCGAATTACCGCGTCTTCACCGACTACGTGGAGCGCCTCGAAGTCATCAAAGGGCCGGCCGCGATGCTA
TATGGCATGTCCCCCAACAGCGGCGTGGGGGGCGTGATCAACATCGTGCCCAAGCGCGCGCACGAGGACCTGGCTTCGGT
GACTGCGAGCTATGCGTCCGACACGCAGTTCGGCGGCCACGTCGACTTGAGCCAGCGCTTCGGCGAATCGAGGGAATGGG
GTGTGCGCTTCAACGGCAGCTACTACCAGGGCGATACACCCATGGATAACCAGTCGCGCAAGGCCGGCATCGGCGCATTG
TCGCTGGACTACCAGGGCAAGCAGCTGCGGGCCTCGCTGGACGTGATTGGCCAGCAGGAAAAGATCGATGCGCCGGGCCG
CGCTTTCATCATGGGGCCCGGCCTGCAGGCCATCCCAGGCGCGCCGGACGGCCGACGCAACGTCACGCAGCCGTGGGAAT
GGACGCGCGGCACTGACGAGTCCCTGCTGTTCCGCACGGAATACGACATCAACGACCACCTCACCGTCTTCGCCGATGCC
GGCGGTGGCTGGACACAGCTTTCGCGCCTGTTCGGCACCACGCCACAAATCGTCAACGCGCAGGGCGACACGTTGAGCAC
GCCCACCTATTACAAGTTCAAGGTCGATCGCGCCACGTACGATGCCGGCTTGCGCAGCCGCTTTGACACCGGCCCCCTGC
GCCATGCCGTGACCTTCCAGGCGAGCGCCTACGCCGACAGCCTGGACCGCGCCAACACCACAGGCACGCCCGTGGCATCG
AATATCTATAACCCCGTGGTACGTCCGGCCCAGGGTCTGCCTGCGCCCGATAGCGTTCCCAAAGTCTCGGATAACGACCA
GACGGGCTTCTCGCTCGCGGACACCATGTCCGCGCTGGATGACCGCGTGCTGTTGACGCTGGGCGTGCGGCAGCAGCGCA
TCCGCTCACGCAATTTCGGCGCAACAGGGGCCGTGACTTCGTCCTATGAGGAAAGCGCCACCACACCGATGGCGGGCCTC
GTCGTGCAGCCATGGAAGCGCGTGTCGCTGTATGCGAACTACGTCCAGGGCTTGAGCAAGGGCGACGTGGCGCCCCCTAC
GGCGAGCAACTCGGGCGAGGTGTTCGCGCCGTATAAGACCACGCAGTACGAGGTCGGCACCAAGGTCGATTTCGGCCGCT
TCAGCGCCACGCTGGCCGCGTTCCAGATCAGCAAGCCCAGCGGCCAGCTCGGCGGCAATGTGTTCTCGGTCAACGGCGAA
CAACGCAACCGCGGGCTGGAGCTGAACCTGTTCGGCCAGGTTGCGCCCGGTGTGCGGCTGTTGGGTGGCGTGGCGCTGAT
CGATGGCGAGATCACGAGGTCCGGCACCGCGGCCGCGGTCGGCAAGGACCCGATCGGCGTGCCGCGCGTTCAGGCCAACC
TTGGTGCGGAATGGGACTTGCCGTGGGTAGCGGGCCTGACCGTAAGCGGCACCATGATCTACACGGGCAGGCAATACGTG
AACCAGCTCAATACCGCCAGCCTGCCGTCATGGGCCAGGTTCGACCTGGGCGCACGCTATCGCACGCGCATCACGGGAAA
GACCACGACCTTCCGCGCCAACGTGCTCAACGTATTCGACCGCAACTACTGGTCGGGTGTGACATCGTGGGGCGGCTTCT
CGCAAGCCGCGCCGCGCACGGTAATGCTGTCCGCCACCGTTGAATTCTGA