SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259592
Component protein type	TssJ
T6SS ID (Type)	T6SS17507 (Type i4a)
Strain	Cupriavidus pinatubonensis JMP134
Replicon	chromosome I
Sequence	Protein sequence (171 a.a.); Nucleotide sequence (513 bp)
Reference	[1] Li C, Zhu L, Wang D, et al. T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer. ISME J. 2022 Feb;16(2):500-510. doi: 10.1038/s41396-021-01093-8. Epub 2021 Aug 25. PMID: 34433898

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	REUT_RS08650
Coordinate (Strand)	1856453..1856965 (-)
NCBI ID	WP_011297881.1
RefSeq	NC_007347
Uniprot ID	Q470V0_CUPPJ
KEGG ID	reu:Reut_A1718
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	MKVVSIWRGIAR
Transmembrane helix	13-35	GAGGLLLAGALAASTVLLGGCAA
Outside	36-170	PSFLSFKGERVKWKQVVLNAADDANGNSPVAVDVVLVSDEALQGRLVDLPASKWFAGRTDLSNTYPGSLRIRSWELVPGQRLEVPPQDFEGPRVTAVFVFANYPEPGAHRVRIDQFSGTLAVQLNSSAVSVSTSK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9274	pos: 32-33. LGG-CA.	MKVVSIWRGIARGAGGLLLAGALAASTVLLGG

Protein sequence: 171 a.a.

						>T6CP259592 NC_007347:c1856965-1856453 [Cupriavidus pinatubonensis JMP134] [TssJ]
MKVVSIWRGIARGAGGLLLAGALAASTVLLGGCAAPSFLSFKGERVKWKQVVLNAADDANGNSPVAVDVVLVSDEALQGR
LVDLPASKWFAGRTDLSNTYPGSLRIRSWELVPGQRLEVPPQDFEGPRVTAVFVFANYPEPGAHRVRIDQFSGTLAVQLN
SSAVSVSTSK*					

Nucleotide sequence: 513 bp

						>T6CP259592 NC_007347:c1856965-1856453 [Cupriavidus pinatubonensis JMP134] [TssJ]
ATGAAGGTAGTGTCGATCTGGCGTGGCATTGCGCGTGGGGCCGGCGGGCTGCTCCTGGCGGGCGCGCTGGCGGCTTCAAC
GGTGCTGCTGGGCGGCTGCGCGGCACCGTCGTTCCTGAGTTTCAAGGGCGAGCGCGTCAAGTGGAAGCAGGTCGTCCTGA
ATGCTGCCGACGACGCCAACGGCAACAGCCCGGTGGCGGTCGACGTGGTGCTGGTCAGCGACGAGGCGCTGCAGGGCCGG
CTGGTCGATCTGCCCGCCTCCAAATGGTTCGCGGGCCGGACCGACCTCAGCAATACCTATCCTGGGAGCCTGCGCATTCG
CAGTTGGGAACTGGTGCCGGGACAGCGCCTGGAAGTCCCGCCCCAGGACTTCGAGGGGCCGCGCGTGACGGCCGTCTTCG
TATTTGCCAACTACCCGGAACCGGGCGCACATCGCGTCCGGATCGATCAATTCAGCGGCACGCTGGCAGTCCAGCTGAAC
AGCAGTGCCGTCAGCGTCTCGACAAGCAAATGA					