Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00114  |
| T6SS type | Type i3 |
| Strain | Burkholderia thailandensis E264 |
| Replicon (RefSeq) | chromosome II (NC_007650) |
| Location | 2282208..2308569 |
| Function | effector translocation |
| Comment | The secretion of BTH_II1884 was completely abrogated in the Δ4clpV mutant lacking four sets of T6SSs (T6SS1, T6SS2, T6SS4, and T6SS6), and the defects in secretion were completely rescued (thus, returned to the wild-type levels) upon complementation of clpV4. These results suggest that BTH_II1884 is a T6SS substrate mainly secreted by T6SS4. |
| References | [1] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] [2] Meiru Si, Yao Wang, Bing Zhang, Chao Zhao, Yiwen Kang, Haonan Bai, Dawei Wei, Lingfang Zhu, Lei Zhang, Tao G Dong, Xihui Shen et al (2017) The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 20(4):949-959. [PudMed:28746878] [3] Schwarz S et al (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170] [4] Meiru Si, Chao Zhao, Brianne Burkinshaw, Bing Zhang, Dawei Wei, Yao Wang, Tao G Dong, Xihui Shen et al (2017) Manganese Scavenging and Oxidative Stress Response Mediated by Type VI Secretion System in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 114(11):E2233-E2242. [PudMed:28242693] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BTH_RS09625 | 2277721..2278998 | - | 1278 | WP_009896562.1 | glycosyltransferase | ||
| BTH_RS09630 | 2279278..2280177 | - | 900 | WP_009896560.1 | alpha/beta hydrolase | ||
| BTH_RS09635 | 2280750..2281025 | - | 276 | WP_009907980.1 | hypothetical protein | ||
| BTH_RS09640 | 2281342..2281767 | - | 426 | WP_011401419.1 | hypothetical protein | ||
| BTH_RS09645 | 2281790..2282149 | - | 360 | WP_009893756.1 | hypothetical protein | ||
| T6CP001072 | TssM | BTH_RS09650 (tssM) | 2282208..2285702 | - | 3495 | WP_009893757.1 | type VI secretion system membrane subunit TssM |
| T6CP001073 | TssL | BTH_RS09655 (icmH) | 2285699..2287426 | - | 1728 | WP_009893758.1 | type IVB secretion system protein IcmH/DotU |
| T6CP001074 | TssK | BTH_RS09660 (tssK) | 2287509..2288870 | - | 1362 | WP_009893759.1 | type VI secretion system baseplate subunit TssK |
| T6CP009346 | TssJ | BTH_RS09665 | 2288867..2289460 | - | 594 | WP_009893760.1 | type VI secretion lipoprotein TssJ |
| BTH_RS09670 | 2289466..2289855 | - | 390 | WP_009893761.1 | DUF4150 domain-containing protein | ||
| BTH_RS09675 | 2289895..2290611 | - | 717 | WP_009893763.1 | DUF3540 domain-containing protein | ||
| BTH_RS09680 | 2290614..2291684 | - | 1071 | WP_009893765.1 | pentapeptide repeat-containing protein | ||
| BTH_RS09685 | 2291684..2293900 | - | 2217 | WP_009893766.1 | pentapeptide repeat-containing protein | ||
| T6CP001075 | TssI | BTH_RS09690 (vgrG) | 2293904..2296192 | - | 2289 | WP_009893768.1 | type VI secretion system tip protein VgrG |
| T6CP001076 | TssI | BTH_RS09695 (vgrG) | 2296361..2298652 | - | 2292 | WP_011401423.1 | type VI secretion system tip protein VgrG |
| T6CP001077 | TssH | BTH_RS09700 (tssH) | 2298643..2301486 | - | 2844 | WP_009893771.1 | type VI secretion system ATPase TssH |
| T6CP001078 | TssG | BTH_RS09705 (tssG) | 2301489..2302478 | - | 990 | WP_009893772.1 | type VI secretion system baseplate subunit TssG |
| T6CP001079 | TssF | BTH_RS09710 (tssF) | 2302475..2304337 | - | 1863 | WP_009901011.1 | type VI secretion system baseplate subunit TssF |
| T6CP001080 | TssE | BTH_RS09715 | 2304351..2304782 | - | 432 | WP_009901012.1 | GPW/gp25 family protein |
| T6CP001081 | TssD | BTH_RS09720 | 2304861..2305388 | - | 528 | WP_009901013.1 | type VI secretion system tube protein Hcp |
| T6CP001082 | TssC | BTH_RS09725 (tssC) | 2305532..2307037 | - | 1506 | WP_009901014.1 | type VI secretion system contractile sheath large subunit |
| T6CP001083 | TssB | BTH_RS09730 (tssB) | 2307040..2307591 | - | 552 | WP_009901015.1 | type VI secretion system contractile sheath small subunit |
| T6CP001084 | TssA | BTH_RS09735 | 2307625..2308569 | - | 945 | WP_009907984.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
| BTH_RS09740 | 2310190..2311704 | - | 1515 | WP_009893778.1 | acetyl-CoA hydrolase/transferase family protein | ||
| BTH_RS09745 | 2312067..2312762 | + | 696 | WP_009893780.1 | CDP-alcohol phosphatidyltransferase family protein | ||
| BTH_RS09750 | 2312759..2313532 | + | 774 | WP_009893782.1 | 2OG-Fe(II) oxygenase family protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0153 |
BTH_I1281 (OxyR) | protein | Global regulator | Burkholderia thailandensis E264 | chromosome (NC_007651) | 1432632..1433591 (+) | 497575020 | BTH_RS09735 (T6SS-4) Promotor,−536 to −186 relative to the ATG start codon (↑) |
| REG0154 |
BTH_I0691 (Zur) | protein | Global regulator | Burkholderia thailandensis E264 | chromosome (NC_007651) | 796074..796538 (-) | 497578547 | BTH_RS09735 (T6SS-4) Promotor,−541 to −1 relative to the ATG start codon (↑) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01464 |
BTH_II1883 (TseM) | Tie | - | Burkholderia thailandensis E264 | chromosome (NC_007650) | 2281342..2281767 (-) | 83718045 |
| EFF01825 |
BTH_II1884 (TseZ) | Tie | - | Burkholderia thailandensis E264 | chromosome (NC_007650) | 2281790..2282149 (-) | 497579572 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|---|---|---|---|---|---|---|---|
| ACP00021 |
BTH_II2139 (hmuR) | chaperone | Burkholderia thailandensis E264 | chromosome (NC_007650) | 2626542..2628827 (+) | 644827063 | - | BTH_II1884 (TseZ) |
| ACP00022 |
BTH_RS20365 (mnoT) | chaperone | Burkholderia thailandensis E264 | chromosome (NC_007651) | 1796067..1798403 (+) | 497575584 | - | BTH_II1883 (TseM) |