Detailed information of regulator
Summary
| Regulator ID | REG0153  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | Biofilm formation - Escherichia coli (map02026) |
| Related T6SS | T6SS00114 (Type i3) |
| Strain | Burkholderia thailandensis E264 |
| Replicon | chromosome |
| Sequence | Protein sequence (320 a.a.); Nucleotide sequence (960 bp) |
| Description | OxyR in chromosome I negatively regulates the expression of T6SS-4 in chromosome II by specifically recognizing an operator within the T6SS-4 promoter. |
| Reference |
External database links
| Locus tag (Gene) | BTH_I1281 (OxyR) |
| Coordinates (Strand) | 1432632..1433591 (+) |
| NCBI ID | 497575020 |
| RefSeq | NC_007651 |
| Uniprot ID | Q2SZ21_BURTA |
| KEGG ID | bte:BTH_I1281 |
| KEGG pathway (determined by BlastKOALA) | map02026 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| BTH_RS09735 (T6SS-4) Promotor,−536 to −186 relative to the ATG start codon | ↑ | cggcgtccgggaaaatattccacggaaaccgtttacgctgctgtttttttctcagtggtattaacccaccccttccgcccagcccgatccctgcgagtcggcatacgccggcttccgcaacggcaatcggtaagatgcactcccgctaaatcaaataccgcacgcgcataaatcgatgcgccgtcgcgatcgcggcggaaataacatccaagcaccgggtttatctgaagcgtcacaccttagtggccctcaacgacaacggcgtgacaatcgttcatatcgttacagtctacgctgtaaacttatagaacgctcgtaaatttggcaagcggaaataattttttcttacaaac |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| LysR_substrate | PF03466.22 | 3.2e-46 | 90..301 |
| HTH_1 | PF00126.29 | 3e-17 | 4..61 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-319 | MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEDELNVQIFERGASEVSVTPIGDQIVTQAQRVLEQTFAIKEIAKQGKDPLIGPFRLGVIYTIGPYLLPTLVKQMIRRVPQMPLMLQENYTLKLLELLKQGEIDAAIMALPFPETGLMVRPLYDEPFVVALPTGHPWGTRRKIDAADLKQETMLLLGNGHCFRDHVLGVCPELMHFSQTADGIQKTFEGSSLETIRHMVASGVGITVLPRMSVAEVGPHASGPDADLLSYVPFEDPVPDRRVVLVWRKSFTRMPAIDAISEAIAACELPGVAKLDIPATVN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9792 | - | - |
Protein sequence of regulator: 320 a.a. .
>REG0153 NC_007651:1432632-1433591 [Burkholderia thailandensis E264]
MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEDELNVQIFERGASEVSVTPIGDQIVTQAQRVLEQTFAIKE
IAKQGKDPLIGPFRLGVIYTIGPYLLPTLVKQMIRRVPQMPLMLQENYTLKLLELLKQGEIDAAIMALPFPETGLMVRPL
YDEPFVVALPTGHPWGTRRKIDAADLKQETMLLLGNGHCFRDHVLGVCPELMHFSQTADGIQKTFEGSSLETIRHMVASG
VGITVLPRMSVAEVGPHASGPDADLLSYVPFEDPVPDRRVVLVWRKSFTRMPAIDAISEAIAACELPGVAKLDIPATVN*
MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEDELNVQIFERGASEVSVTPIGDQIVTQAQRVLEQTFAIKE
IAKQGKDPLIGPFRLGVIYTIGPYLLPTLVKQMIRRVPQMPLMLQENYTLKLLELLKQGEIDAAIMALPFPETGLMVRPL
YDEPFVVALPTGHPWGTRRKIDAADLKQETMLLLGNGHCFRDHVLGVCPELMHFSQTADGIQKTFEGSSLETIRHMVASG
VGITVLPRMSVAEVGPHASGPDADLLSYVPFEDPVPDRRVVLVWRKSFTRMPAIDAISEAIAACELPGVAKLDIPATVN*
Nucleotide sequence of regulator: 960 bp .
>REG0153 NC_007651:1432632-1433591 [Burkholderia thailandensis E264]
ATGACGCTTACTGAACTGAAGTACATCGTCGCGGTCGCGCGCGAGCGCCATTTCGGCCGGGCGGCCGAAGCCTGCTTCGT
CAGCCAGCCGACGCTGTCGGTCGCGATCAAGAAGCTGGAAGACGAGTTGAACGTGCAGATCTTCGAGCGCGGCGCGAGCG
AGGTGAGCGTGACGCCGATCGGCGACCAGATCGTCACGCAAGCGCAGCGCGTGCTCGAGCAGACGTTCGCGATCAAGGAG
ATCGCGAAGCAGGGCAAGGATCCGCTCATCGGGCCGTTTCGGCTCGGCGTGATCTATACGATCGGGCCCTATCTGCTGCC
GACGCTCGTCAAGCAGATGATTCGCCGCGTGCCGCAAATGCCGTTGATGCTGCAGGAGAACTACACGCTGAAGCTGCTCG
AGCTGCTCAAACAGGGCGAGATCGACGCGGCGATCATGGCATTGCCGTTTCCCGAAACCGGCCTGATGGTGCGGCCGCTG
TACGACGAGCCGTTCGTCGTCGCGCTGCCCACCGGGCATCCATGGGGGACGCGCCGCAAGATCGACGCGGCCGACCTGAA
GCAGGAGACGATGCTGCTGCTCGGCAACGGCCATTGCTTCCGCGACCACGTGCTCGGCGTATGTCCGGAGCTGATGCATT
TTTCGCAGACGGCGGACGGCATTCAGAAGACGTTCGAAGGCTCGTCGCTCGAGACGATCCGCCACATGGTCGCGAGCGGC
GTCGGGATCACGGTGCTGCCGCGGATGTCGGTCGCCGAGGTGGGGCCGCATGCAAGCGGACCGGACGCGGACCTGCTGTC
CTACGTGCCGTTCGAGGACCCGGTGCCGGATCGGCGCGTCGTGCTCGTGTGGCGCAAGAGCTTCACGCGGATGCCGGCGA
TCGACGCGATCAGCGAGGCGATCGCCGCATGCGAGTTGCCCGGCGTGGCGAAGCTCGACATCCCGGCTACGGTGAATTGA
ATGACGCTTACTGAACTGAAGTACATCGTCGCGGTCGCGCGCGAGCGCCATTTCGGCCGGGCGGCCGAAGCCTGCTTCGT
CAGCCAGCCGACGCTGTCGGTCGCGATCAAGAAGCTGGAAGACGAGTTGAACGTGCAGATCTTCGAGCGCGGCGCGAGCG
AGGTGAGCGTGACGCCGATCGGCGACCAGATCGTCACGCAAGCGCAGCGCGTGCTCGAGCAGACGTTCGCGATCAAGGAG
ATCGCGAAGCAGGGCAAGGATCCGCTCATCGGGCCGTTTCGGCTCGGCGTGATCTATACGATCGGGCCCTATCTGCTGCC
GACGCTCGTCAAGCAGATGATTCGCCGCGTGCCGCAAATGCCGTTGATGCTGCAGGAGAACTACACGCTGAAGCTGCTCG
AGCTGCTCAAACAGGGCGAGATCGACGCGGCGATCATGGCATTGCCGTTTCCCGAAACCGGCCTGATGGTGCGGCCGCTG
TACGACGAGCCGTTCGTCGTCGCGCTGCCCACCGGGCATCCATGGGGGACGCGCCGCAAGATCGACGCGGCCGACCTGAA
GCAGGAGACGATGCTGCTGCTCGGCAACGGCCATTGCTTCCGCGACCACGTGCTCGGCGTATGTCCGGAGCTGATGCATT
TTTCGCAGACGGCGGACGGCATTCAGAAGACGTTCGAAGGCTCGTCGCTCGAGACGATCCGCCACATGGTCGCGAGCGGC
GTCGGGATCACGGTGCTGCCGCGGATGTCGGTCGCCGAGGTGGGGCCGCATGCAAGCGGACCGGACGCGGACCTGCTGTC
CTACGTGCCGTTCGAGGACCCGGTGCCGGATCGGCGCGTCGTGCTCGTGTGGCGCAAGAGCTTCACGCGGATGCCGGCGA
TCGACGCGATCAGCGAGGCGATCGCCGCATGCGAGTTGCCCGGCGTGGCGAAGCTCGACATCCCGGCTACGGTGAATTGA