Detailed information of component protein


Summary

Component ID T6CP001073
Component protein type TssL
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (576 a.a.); Nucleotide sequence (1728 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09655
Coordinate (Strand) 2285699..2287426 (-)
NCBI ID WP_009893758.1
RefSeq NC_007650
Uniprot ID UPI00016A3DA0
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 5.6e-42 121..316
OmpA PF00691.22 6.9e-09 376..483
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-294 MLDRAAANPDLVTRVPTLSSLTSAAMTSSAVSTTGTTNVAASGGAASGMGVPGAYPAADVPHADGAPRNPAVLQFPVPGGAHAPADARDAAPVVYSAQGEQAAIMKAGLQQASWNNPFVSHALPAVLQLQRHLAAGPLNQAAIRTQLGLEVRLYRERLAGSGCEWEQIRDASYLLCTYLDETVNDSAREHSQVVYDGERSLLVEFHDDAWGGEDAFADLSRWMKADQPPIPLLSFYELILSLGWQGRYRVLDRGDVLLQDLRSQLHALIWHHVPPEPLGTDLATPAKRRRSWWT
Transmembrane helix 295-317 AGRAAAVALGVLVLAYGAISLWL
Outside 318-575 DSQGRPIRNALAAWMPPTRTINIAETLPPPLPQILTEGWLTAYKHPQGWLLVFKSDGAFDVGKAKVRPDFMHNIERLGLAFAPWPGDLEVIGHTDSRPIRTSEFPDNQALSEARARTVADELRATALPGGARAPENAVQRNIEYSGRGDAQPIDTAKTAAAYERNRRVDVLWKVIPDGASQPGRSLNLQQPEKPGQVPMRPAMPEGVEIAPEGQLPYATTEITTATPTTMPTTKQAPTPVPTPAPTPATGPTTEGRQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
TAT(Tat/SPI) 0.5063 pos: 41-42. VAA-SG. MLDRAAANPDLVTRVPTLSSLTSAAMTSSAVSTTGTTNVAA


  Protein sequence: 576 a.a.    

>T6CP001073 NC_007650:c2287426-2285699 [Burkholderia thailandensis E264] [TssL]
MLDRAAANPDLVTRVPTLSSLTSAAMTSSAVSTTGTTNVAASGGAASGMGVPGAYPAADVPHADGAPRNPAVLQFPVPGG
AHAPADARDAAPVVYSAQGEQAAIMKAGLQQASWNNPFVSHALPAVLQLQRHLAAGPLNQAAIRTQLGLEVRLYRERLAG
SGCEWEQIRDASYLLCTYLDETVNDSAREHSQVVYDGERSLLVEFHDDAWGGEDAFADLSRWMKADQPPIPLLSFYELIL
SLGWQGRYRVLDRGDVLLQDLRSQLHALIWHHVPPEPLGTDLATPAKRRRSWWTAGRAAAVALGVLVLAYGAISLWLDSQ
GRPIRNALAAWMPPTRTINIAETLPPPLPQILTEGWLTAYKHPQGWLLVFKSDGAFDVGKAKVRPDFMHNIERLGLAFAP
WPGDLEVIGHTDSRPIRTSEFPDNQALSEARARTVADELRATALPGGARAPENAVQRNIEYSGRGDAQPIDTAKTAAAYE
RNRRVDVLWKVIPDGASQPGRSLNLQQPEKPGQVPMRPAMPEGVEIAPEGQLPYATTEITTATPTTMPTTKQAPTPVPTP
APTPATGPTTEGRQP*

  Nucleotide sequence: 1728 bp    

>T6CP001073 NC_007650:c2287426-2285699 [Burkholderia thailandensis E264] [TssL]
ATGCTCGATCGCGCGGCGGCGAATCCGGATCTCGTGACGCGCGTTCCGACGCTGTCGTCGCTGACCAGCGCGGCGATGAC
GAGCTCCGCCGTATCGACCACCGGCACGACGAACGTGGCTGCGTCGGGTGGCGCTGCGTCGGGCATGGGCGTGCCGGGCG
CATATCCGGCCGCCGACGTGCCGCATGCCGACGGTGCGCCCCGCAATCCGGCGGTGCTGCAATTTCCGGTGCCGGGCGGC
GCGCATGCGCCTGCCGACGCGCGGGACGCGGCACCCGTCGTCTACAGCGCGCAGGGCGAGCAGGCCGCGATCATGAAGGC
GGGCCTGCAGCAGGCGAGCTGGAACAATCCGTTCGTGTCGCACGCGCTGCCCGCCGTGCTGCAGTTGCAGCGGCATCTCG
CGGCCGGGCCGCTCAATCAGGCCGCGATTCGCACGCAGCTCGGGCTCGAAGTGCGGCTCTACCGCGAGCGGCTCGCCGGC
TCCGGCTGCGAATGGGAGCAGATCCGCGATGCGTCGTACCTGTTGTGCACGTATCTCGACGAGACCGTCAACGATTCGGC
GCGCGAGCATTCGCAGGTCGTCTATGACGGCGAGCGCAGTCTGCTCGTCGAGTTTCACGACGACGCATGGGGCGGCGAGG
ATGCGTTCGCCGACCTGTCGCGCTGGATGAAGGCGGACCAGCCGCCGATTCCGCTTCTGTCGTTCTACGAACTGATCCTG
TCGCTCGGCTGGCAGGGGCGCTACCGCGTGCTCGACCGCGGCGACGTGCTGCTGCAGGATCTGCGCTCGCAACTGCACGC
GCTGATCTGGCATCACGTGCCGCCCGAGCCGCTCGGCACCGATCTCGCGACGCCCGCGAAACGGCGCCGTTCGTGGTGGA
CGGCCGGCCGCGCGGCGGCCGTCGCGCTCGGCGTGCTCGTGCTCGCGTACGGCGCGATCAGCCTCTGGCTCGATTCGCAG
GGGCGGCCGATCCGCAACGCGCTCGCCGCGTGGATGCCGCCGACGCGCACGATCAACATCGCCGAGACGCTGCCGCCGCC
GCTGCCGCAGATCCTCACCGAAGGGTGGCTCACCGCGTACAAGCATCCGCAGGGATGGCTGCTCGTGTTCAAGAGCGACG
GCGCGTTCGACGTCGGCAAGGCGAAGGTCCGGCCGGACTTCATGCACAACATCGAGCGGCTCGGCCTCGCGTTCGCGCCC
TGGCCGGGCGACCTCGAGGTGATCGGCCACACCGATTCGCGGCCGATCCGCACGAGCGAGTTCCCGGACAACCAGGCGCT
GTCGGAGGCGCGGGCGCGCACGGTGGCCGACGAGCTGCGCGCGACCGCGCTGCCGGGCGGCGCGCGTGCGCCGGAGAACG
CGGTGCAGCGCAACATCGAGTATTCGGGGCGCGGCGACGCGCAACCGATCGACACCGCGAAGACGGCCGCCGCGTACGAG
CGCAACCGCCGCGTCGACGTGCTGTGGAAGGTGATTCCAGACGGTGCGTCGCAACCGGGCCGCAGCCTGAATCTGCAGCA
GCCGGAGAAGCCCGGCCAAGTGCCGATGCGTCCGGCGATGCCGGAAGGCGTCGAGATCGCGCCTGAAGGGCAATTGCCGT
ATGCGACCACGGAGATCACGACCGCGACGCCGACCACGATGCCAACCACGAAGCAAGCACCGACACCGGTGCCGACACCA
GCACCGACGCCAGCAACGGGACCGACCACGGAGGGCCGTCAGCCATGA