Detailed information of component protein


Summary

Component ID T6CP001072
Component protein type TssM
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (1165 a.a.); Nucleotide sequence (3495 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09650
Coordinate (Strand) 2282208..2285702 (-)
NCBI ID WP_009893757.1
RefSeq NC_007650
Uniprot ID Q2T420_BURTA, A0A7X3J5Y1_BURTH
KEGG ID bte:BTH_II1885
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.4e-63 493..795
IcmF-related_N PF14331.8 1.2e-29 192..378
IcmF_C PF06744.14 6.9e-17 1043..1143
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 MIRTSLRV
Transmembrane helix 9-31 FAAILIAILIWWVGPLFAFGIYH
Outside 32-40 PLGPVWVRE
Transmembrane helix 41-60 ILVALVLIWGFWPTLARLWA
Inside 61-431 RLAMSPRQVKVAPKTKQLDFVDKHLRNLDQQLKERWRKEPRGRWKRWVGTLTREHRAMLPWYLVLGSEGSGKTSLVAKAVSVSGSLQDRVLGSDATYGRGDDFNFRITREAVWFDVGGRWSLRAGADEAEFDAWRKLLRGMRRLRKGAPISGVVLCVDGLDMVDAPLDARKRLADSVRARLEEMREAFGQQVQVYVALNGLDRLDGAVSTLSLLDASKWAKGVGFSLPDDGAEADAARADANWQHALQGLQQRVQQQVLYSAPAATEVSMNHAQLRFVETLSRLQKALVAWLHVALAPGEPHTAARLRGVWLGSMAELAEAHPAGVGSSELPVPSRPLSELWTPLIRQVSLERDAVRPSGPKSWRGRLGEA
Transmembrane helix 432-454 LRWGAVPLVALSLLLWFGWGYVT
Outside 455-1164 ERDYLDGVWAQFTEAKRLAQAEASYGNDGGSALIEIANQMRYAQLQAEDAAQGMATPYFEHGLVAETARETYYRHLQKMLMPELYNEVRRTLVSQVDGTPGDIYQTLKVYLMLCRPDRRSADDVVRWLDGRWDALSGGQYSDDDRRSLLGHVRTLMSLKNVPATPEDANLVRSARAKAAQIPLVTRVLQHIHAQGLPQQVNDISLSRAAGFEASMSLRMRSNVPSTDTAVSGWFTRAGYTDVFLPRLQKSARAMLEEESWVLRDETLSGNSFQIDGLVQKLADSARNQFLQDYISAWQNFLNDVTVRGVTGLDDASQLAAAMMEAQSPLANLLRFAARETTLTGASDEGNIDSWIDRQKYRFEKGRRQIVGELSGQHYRTVLLPEHVVEEHFQAIRQLAAQLNRNNTIANNPLSRLFEPLYRQLGLVNGALQAGQVLPAQYDAFSRLKETAARQPEPVRGIMLDLVSSGSTMTTRESGALLNRGAAGATKMVCDQGFTGRYPMRRNAQADAGVEDFERLFSAQGLMATYFRDHLAAYVDTSAKPWQALRSNGGPNGMVSQSVLNSYETADRIRGAMLDDSGHLRVSTVLRFIDMDSQLSEAQLSVAGQTVRFAHGVTSPHRVDWTNQNTQLAIKLQLKSVDGRMTTLQFDGPWALFRFFDAGQAVGGTGTADRRERLYQTSLGTVRIEWQALTLPSPIWSGILQSFRCPS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9979 - -


  Protein sequence: 1165 a.a.    

>T6CP001072 NC_007650:c2285702-2282208 [Burkholderia thailandensis E264] [TssM]
MIRTSLRVFAAILIAILIWWVGPLFAFGIYHPLGPVWVREILVALVLIWGFWPTLARLWARLAMSPRQVKVAPKTKQLDF
VDKHLRNLDQQLKERWRKEPRGRWKRWVGTLTREHRAMLPWYLVLGSEGSGKTSLVAKAVSVSGSLQDRVLGSDATYGRG
DDFNFRITREAVWFDVGGRWSLRAGADEAEFDAWRKLLRGMRRLRKGAPISGVVLCVDGLDMVDAPLDARKRLADSVRAR
LEEMREAFGQQVQVYVALNGLDRLDGAVSTLSLLDASKWAKGVGFSLPDDGAEADAARADANWQHALQGLQQRVQQQVLY
SAPAATEVSMNHAQLRFVETLSRLQKALVAWLHVALAPGEPHTAARLRGVWLGSMAELAEAHPAGVGSSELPVPSRPLSE
LWTPLIRQVSLERDAVRPSGPKSWRGRLGEALRWGAVPLVALSLLLWFGWGYVTERDYLDGVWAQFTEAKRLAQAEASYG
NDGGSALIEIANQMRYAQLQAEDAAQGMATPYFEHGLVAETARETYYRHLQKMLMPELYNEVRRTLVSQVDGTPGDIYQT
LKVYLMLCRPDRRSADDVVRWLDGRWDALSGGQYSDDDRRSLLGHVRTLMSLKNVPATPEDANLVRSARAKAAQIPLVTR
VLQHIHAQGLPQQVNDISLSRAAGFEASMSLRMRSNVPSTDTAVSGWFTRAGYTDVFLPRLQKSARAMLEEESWVLRDET
LSGNSFQIDGLVQKLADSARNQFLQDYISAWQNFLNDVTVRGVTGLDDASQLAAAMMEAQSPLANLLRFAARETTLTGAS
DEGNIDSWIDRQKYRFEKGRRQIVGELSGQHYRTVLLPEHVVEEHFQAIRQLAAQLNRNNTIANNPLSRLFEPLYRQLGL
VNGALQAGQVLPAQYDAFSRLKETAARQPEPVRGIMLDLVSSGSTMTTRESGALLNRGAAGATKMVCDQGFTGRYPMRRN
AQADAGVEDFERLFSAQGLMATYFRDHLAAYVDTSAKPWQALRSNGGPNGMVSQSVLNSYETADRIRGAMLDDSGHLRVS
TVLRFIDMDSQLSEAQLSVAGQTVRFAHGVTSPHRVDWTNQNTQLAIKLQLKSVDGRMTTLQFDGPWALFRFFDAGQAVG
GTGTADRRERLYQTSLGTVRIEWQALTLPSPIWSGILQSFRCPS*

  Nucleotide sequence: 3495 bp    

>T6CP001072 NC_007650:c2285702-2282208 [Burkholderia thailandensis E264] [TssM]
ATGATCCGCACGAGCTTGAGAGTATTCGCGGCGATTCTGATCGCGATCCTGATCTGGTGGGTGGGGCCGCTCTTCGCGTT
CGGCATCTATCACCCGCTCGGCCCCGTGTGGGTGCGCGAGATCCTCGTCGCGCTCGTGCTGATCTGGGGCTTCTGGCCGA
CGCTCGCGCGGCTCTGGGCGCGGCTCGCGATGAGCCCGCGGCAGGTGAAGGTCGCGCCGAAGACGAAGCAGCTCGATTTC
GTCGACAAGCATCTGCGCAACCTCGACCAGCAGTTGAAGGAACGCTGGCGCAAGGAGCCGCGCGGCCGCTGGAAGCGCTG
GGTCGGCACGCTCACGCGCGAGCATCGCGCGATGCTGCCGTGGTATCTGGTGCTCGGCTCCGAGGGCAGCGGCAAGACGA
GCCTCGTCGCGAAGGCGGTCAGCGTGTCCGGCTCGCTGCAGGATCGCGTACTCGGCTCGGACGCGACCTACGGGCGCGGC
GACGACTTCAACTTCCGGATCACGCGCGAGGCCGTGTGGTTCGACGTCGGCGGCCGCTGGAGCCTGCGTGCGGGCGCGGA
CGAGGCCGAGTTCGACGCGTGGCGCAAGCTGCTGCGCGGGATGAGGCGGCTGCGCAAGGGCGCCCCGATCAGCGGCGTCG
TGCTGTGCGTCGACGGCCTCGACATGGTCGATGCGCCGCTCGACGCGCGCAAGCGCCTGGCCGATTCGGTGCGCGCGCGG
CTCGAGGAAATGCGCGAGGCGTTCGGCCAGCAGGTGCAGGTGTATGTCGCGCTGAACGGGCTCGACCGGCTCGACGGCGC
GGTGTCGACGCTGTCGCTGCTCGACGCGTCGAAGTGGGCGAAGGGCGTCGGCTTCAGCCTGCCCGACGACGGCGCGGAAG
CCGACGCGGCGCGCGCGGACGCGAACTGGCAGCACGCGCTGCAAGGGCTGCAGCAGCGTGTGCAGCAGCAAGTGCTGTAT
TCGGCGCCGGCCGCGACCGAGGTGTCGATGAACCACGCGCAACTGCGCTTCGTCGAGACGCTCAGCCGGCTGCAGAAGGC
GCTCGTCGCGTGGCTGCACGTCGCGCTCGCGCCGGGCGAGCCGCACACGGCCGCGCGGCTGCGCGGCGTGTGGCTCGGTT
CGATGGCCGAGCTCGCGGAGGCGCATCCGGCGGGCGTCGGCTCGTCGGAGCTTCCCGTGCCGTCCCGGCCGCTCAGCGAG
CTGTGGACGCCGCTCATCCGGCAGGTGTCGCTCGAGCGCGATGCGGTGCGCCCGAGCGGCCCGAAGTCGTGGCGCGGCCG
CCTGGGCGAGGCGCTGCGATGGGGCGCGGTGCCCCTCGTCGCGCTGAGCCTCCTGCTGTGGTTCGGCTGGGGCTACGTCA
CCGAGCGCGATTATCTGGACGGCGTGTGGGCGCAGTTCACCGAGGCGAAGCGGCTCGCGCAGGCCGAGGCGTCCTACGGC
AACGACGGCGGCTCGGCGCTCATCGAGATCGCGAACCAGATGCGCTACGCGCAGTTGCAGGCCGAGGACGCCGCGCAGGG
GATGGCCACGCCGTACTTCGAGCACGGGCTCGTCGCCGAGACCGCGCGCGAGACCTACTACCGGCACTTACAGAAGATGC
TGATGCCGGAGCTGTACAACGAGGTGCGGCGCACGCTCGTGTCGCAGGTCGACGGCACCCCGGGCGACATCTATCAGACG
CTGAAGGTCTACCTGATGCTCTGCCGTCCTGATCGACGCTCGGCGGACGACGTCGTGCGCTGGCTCGACGGCCGGTGGGA
CGCGCTATCGGGCGGCCAGTATTCGGACGACGACCGCCGCTCGCTGCTTGGGCATGTGCGCACGCTGATGTCGCTGAAGA
ACGTGCCGGCGACGCCGGAGGACGCGAACCTCGTGCGCTCGGCGCGTGCGAAGGCCGCGCAGATCCCGCTCGTCACGCGC
GTGCTGCAGCACATCCATGCGCAGGGGCTGCCGCAGCAGGTCAACGACATCTCGCTGTCGCGCGCGGCGGGCTTCGAGGC
GTCGATGAGCCTGCGCATGCGCAGCAACGTGCCGTCGACGGACACCGCGGTCTCCGGCTGGTTCACGCGCGCCGGCTACA
CGGATGTGTTCCTGCCGCGCCTGCAGAAGAGCGCGCGCGCGATGCTCGAGGAGGAGAGCTGGGTGCTGCGCGACGAGACG
CTGTCGGGCAACAGCTTCCAGATCGACGGACTCGTGCAGAAGCTCGCCGATTCGGCGCGCAACCAGTTCCTTCAGGATTA
CATCTCCGCATGGCAGAACTTCCTGAACGACGTGACGGTGCGCGGCGTGACGGGCCTCGACGACGCGTCGCAGCTCGCGG
CCGCGATGATGGAGGCGCAATCGCCGCTCGCGAACCTGCTGCGCTTCGCCGCGCGCGAGACGACGCTCACGGGCGCGAGC
GACGAGGGCAACATCGACAGCTGGATCGATCGCCAGAAGTACCGCTTCGAGAAGGGGCGGCGGCAGATCGTCGGCGAGCT
GAGCGGGCAGCATTACCGCACGGTGCTGCTGCCGGAGCACGTGGTCGAGGAGCACTTCCAGGCGATCCGCCAGCTCGCCG
CGCAGTTGAACCGCAACAACACGATTGCGAACAATCCGCTGTCGCGCCTGTTCGAGCCGCTGTACCGGCAGCTCGGGCTC
GTCAACGGCGCGCTGCAGGCGGGCCAGGTGCTGCCCGCGCAGTACGACGCGTTCTCGCGGCTCAAGGAGACGGCCGCGCG
TCAGCCGGAGCCCGTGCGCGGGATCATGCTCGACCTCGTGAGCAGCGGCAGCACGATGACGACGCGCGAATCGGGCGCGC
TGCTCAATCGCGGCGCGGCGGGCGCGACGAAGATGGTCTGCGATCAGGGCTTCACGGGCCGCTATCCGATGCGCCGCAAC
GCGCAGGCGGATGCGGGCGTCGAGGACTTCGAACGATTGTTCAGCGCGCAAGGGCTGATGGCGACGTACTTCCGCGATCA
CCTCGCCGCGTATGTCGACACGTCCGCGAAGCCGTGGCAGGCGCTGCGCTCGAACGGCGGGCCGAACGGGATGGTCAGTC
AGTCCGTCCTGAACTCGTACGAGACGGCAGATCGCATCCGCGGCGCGATGCTCGACGATTCGGGGCACCTGCGCGTGTCG
ACCGTGCTGCGCTTCATCGACATGGATTCGCAGTTGTCCGAAGCGCAGTTGAGCGTCGCCGGCCAGACAGTGCGCTTCGC
GCACGGCGTGACGTCGCCGCACCGGGTCGACTGGACCAATCAGAACACGCAGCTCGCGATCAAGCTGCAGCTGAAGTCCG
TCGACGGCCGGATGACGACGCTGCAGTTCGACGGGCCGTGGGCGCTGTTCCGCTTCTTCGACGCGGGACAGGCGGTGGGC
GGCACGGGCACGGCGGACCGGCGCGAGCGGCTCTATCAGACGAGCCTCGGCACGGTCCGGATCGAATGGCAGGCGCTCAC
GCTGCCGTCGCCGATATGGTCGGGCATCCTGCAGTCGTTCAGGTGTCCGTCGTGA