Detailed information of component protein


Summary

Component ID T6CP001077
Component protein type TssH
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (948 a.a.); Nucleotide sequence (2844 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09700
Coordinate (Strand) 2298643..2301486 (-)
NCBI ID WP_009893771.1
RefSeq NC_007650
Uniprot ID Q2T410_BURTA, A0A7X3DW19_BURTH
KEGG ID bte:BTH_II1895
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 3.2e-33 597..763
AAA_lid_9 PF17871.3 4.5e-26 368..449
AAA PF00004.31 2.9e-08 228..333
ClpB_D2-small PF10431.11 5.9e-06 769..842
Clp_N PF02861.22 7e-05 23..72
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-947 VNRERIFNCLGRTTYAALVDATALGRSRRHAFIDLDHWALCLLQREQSDLARLFEQFGSDVGEAKRRMEKALDGFDVSGDSLRDISSSLERSVGPAVIWSQIAARAGKVRSGHLLLAWLDEDLTRRWLQQRVPNGITSVALDEVVKRYEALAPGWTETDEAPAALDGGALGAEAGEAGADGTSDALAKWATCVTEQAARGELDPVVGRDDELRTVIDILSRRRQNNPILVGEAGVGKTAVVEALAQKIHAGAVPPGLIGAQVWALDLARMQAGAGVRGEFEQRLKSLIDAVIASAAPIILFCDETHTLIGAGGAAGTGDAANLIKPMLARGQLRMVAATTWSEYKQYIEPDAALVRRFQAVPVDEPSDDAAVDMLRTIAPRFAAHHGVRIVDSALRGAVELSRRYLPARQLPDKAISLLDTACARVAMSQNCAPAELERLQHQAFAIGQTLDWRASDRRMGVRTPGDEAELEGRQASLVQQAATLETVVDAQRDEVRSWLARLNDETHVSADSDGDSFAARIGANRWVRPWVDEHVVSEVLAEWTGVPVAQLAQDDAQRVVELEGALNAGIHGQTGAMRSIAQALQVSHSGLNDPRRPLGVMLLAGPTGTGKSQAAAKLAELLFGGERNLLQFNMNEFQEAHTVSTLKGAPPGYVGYGKGGRLTEAVRKKPYSVLLLDEFDRAHPDIHEVFYQVFDQGWMEDGEGRRISFRNCLILLTSNLGDVEIEAACKADPQISQAKLDKLVGERLQGRFSPALLARIQLVAFRPLDVDALTGIATQALDELGERLAENDLQWRADEGVASWVAHAVSQHPANGRAVRDLLRQHVMPAVARGVLAARAEGRALKTVRLSAHEKLSLVFDEDALEIGGTDAASLGEQAEAAAREAAASAAAHGAPEAHDAPESRDVHEANGDMRAEADDERPADAHHPDETTSANAGTTGEPSCV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9333 - -


  Protein sequence: 948 a.a.    

>T6CP001077 NC_007650:c2301486-2298643 [Burkholderia thailandensis E264] [TssH]
VNRERIFNCLGRTTYAALVDATALGRSRRHAFIDLDHWALCLLQREQSDLARLFEQFGSDVGEAKRRMEKALDGFDVSGD
SLRDISSSLERSVGPAVIWSQIAARAGKVRSGHLLLAWLDEDLTRRWLQQRVPNGITSVALDEVVKRYEALAPGWTETDE
APAALDGGALGAEAGEAGADGTSDALAKWATCVTEQAARGELDPVVGRDDELRTVIDILSRRRQNNPILVGEAGVGKTAV
VEALAQKIHAGAVPPGLIGAQVWALDLARMQAGAGVRGEFEQRLKSLIDAVIASAAPIILFCDETHTLIGAGGAAGTGDA
ANLIKPMLARGQLRMVAATTWSEYKQYIEPDAALVRRFQAVPVDEPSDDAAVDMLRTIAPRFAAHHGVRIVDSALRGAVE
LSRRYLPARQLPDKAISLLDTACARVAMSQNCAPAELERLQHQAFAIGQTLDWRASDRRMGVRTPGDEAELEGRQASLVQ
QAATLETVVDAQRDEVRSWLARLNDETHVSADSDGDSFAARIGANRWVRPWVDEHVVSEVLAEWTGVPVAQLAQDDAQRV
VELEGALNAGIHGQTGAMRSIAQALQVSHSGLNDPRRPLGVMLLAGPTGTGKSQAAAKLAELLFGGERNLLQFNMNEFQE
AHTVSTLKGAPPGYVGYGKGGRLTEAVRKKPYSVLLLDEFDRAHPDIHEVFYQVFDQGWMEDGEGRRISFRNCLILLTSN
LGDVEIEAACKADPQISQAKLDKLVGERLQGRFSPALLARIQLVAFRPLDVDALTGIATQALDELGERLAENDLQWRADE
GVASWVAHAVSQHPANGRAVRDLLRQHVMPAVARGVLAARAEGRALKTVRLSAHEKLSLVFDEDALEIGGTDAASLGEQA
EAAAREAAASAAAHGAPEAHDAPESRDVHEANGDMRAEADDERPADAHHPDETTSANAGTTGEPSCV*

  Nucleotide sequence: 2844 bp    

>T6CP001077 NC_007650:c2301486-2298643 [Burkholderia thailandensis E264] [TssH]
GTGAATCGCGAACGCATATTCAACTGTCTCGGCCGCACGACCTATGCGGCCCTTGTCGATGCGACGGCGCTAGGCCGATC
CCGTCGGCATGCCTTCATCGATCTCGATCACTGGGCACTGTGCCTGCTGCAGCGCGAGCAGAGCGATCTCGCGCGGCTGT
TCGAGCAGTTCGGCAGCGATGTCGGCGAAGCGAAGCGCCGGATGGAGAAGGCGCTGGACGGCTTCGACGTGAGCGGCGAC
TCGCTGCGCGACATCTCCAGCTCGCTCGAGCGCAGCGTCGGGCCGGCCGTCATCTGGAGCCAGATCGCGGCGCGCGCGGG
CAAGGTGCGCTCCGGCCACCTGTTGCTCGCGTGGCTCGACGAGGATCTGACGCGCCGCTGGTTGCAGCAGCGCGTGCCGA
ACGGCATCACGTCGGTCGCGCTCGACGAGGTGGTGAAGCGCTATGAGGCGCTCGCGCCCGGCTGGACGGAAACCGACGAA
GCGCCCGCGGCGCTCGACGGCGGCGCGCTTGGCGCGGAAGCGGGCGAGGCCGGCGCCGACGGCACGAGCGACGCGCTCGC
GAAATGGGCGACGTGCGTGACCGAGCAGGCCGCGCGCGGCGAGCTCGATCCCGTGGTCGGCCGCGACGATGAATTGCGCA
CGGTGATCGACATCCTGTCGCGCCGCCGCCAGAACAACCCGATTCTCGTCGGCGAGGCAGGCGTGGGCAAGACGGCCGTC
GTCGAGGCGCTCGCGCAGAAGATCCACGCGGGCGCGGTGCCGCCGGGCCTCATCGGCGCGCAGGTGTGGGCGCTCGATCT
GGCGCGGATGCAGGCGGGCGCGGGCGTGCGCGGCGAGTTCGAGCAGCGCCTGAAATCGCTGATCGACGCGGTGATCGCGT
CGGCCGCGCCGATCATCCTGTTCTGCGACGAGACGCATACGCTGATCGGCGCGGGCGGCGCGGCCGGCACGGGCGACGCG
GCGAACCTGATCAAGCCGATGCTCGCGCGCGGCCAGTTGCGGATGGTCGCCGCGACGACGTGGTCCGAATACAAGCAGTA
CATCGAGCCGGATGCGGCGCTCGTGCGGCGCTTCCAGGCTGTTCCGGTCGACGAGCCGAGCGACGACGCGGCGGTCGACA
TGCTGCGCACGATCGCGCCGCGCTTCGCGGCGCACCACGGCGTGCGGATCGTGGATTCGGCGCTGCGCGGCGCGGTCGAG
CTGTCGCGCCGCTATCTGCCCGCGCGGCAGTTGCCGGACAAGGCGATCAGCCTGCTCGACACCGCGTGCGCGCGCGTCGC
GATGAGTCAGAACTGCGCGCCCGCCGAGCTCGAGCGCTTGCAGCATCAGGCGTTCGCGATCGGCCAGACGCTCGATTGGC
GTGCGAGCGACCGCCGCATGGGCGTGCGCACGCCGGGCGACGAAGCCGAGCTCGAGGGCCGCCAGGCGAGCCTCGTCCAG
CAGGCGGCCACGCTCGAGACGGTCGTCGACGCGCAGCGCGACGAAGTGCGTTCGTGGCTCGCGCGTCTGAACGACGAGAC
GCATGTGTCTGCCGACAGCGACGGCGATTCGTTCGCCGCGCGCATCGGCGCGAATCGCTGGGTGCGGCCGTGGGTCGACG
AGCACGTCGTGTCGGAGGTGCTCGCCGAGTGGACGGGCGTGCCCGTCGCGCAGCTCGCGCAGGACGACGCGCAGCGCGTG
GTCGAGCTCGAGGGGGCGCTCAATGCGGGCATCCACGGGCAGACGGGCGCGATGCGCTCGATCGCGCAGGCGCTGCAGGT
GTCGCATTCGGGGCTCAACGATCCGCGCCGGCCGCTCGGCGTGATGCTGCTCGCCGGCCCGACCGGCACCGGCAAGAGCC
AGGCCGCGGCGAAGCTCGCCGAGCTGCTGTTCGGCGGCGAGCGCAACCTGCTGCAGTTCAACATGAACGAGTTTCAGGAA
GCGCACACGGTGTCGACGCTCAAGGGCGCGCCGCCCGGATACGTCGGTTACGGCAAGGGCGGGCGCTTGACCGAGGCGGT
TCGCAAGAAGCCGTACAGCGTGCTGCTGCTCGACGAATTCGACCGCGCGCATCCGGACATTCATGAGGTGTTCTATCAAG
TCTTCGATCAGGGCTGGATGGAGGATGGCGAGGGCCGTCGGATCAGCTTCCGCAACTGCCTGATCCTGTTGACGAGCAAT
CTCGGCGACGTCGAGATCGAAGCGGCGTGCAAGGCCGATCCGCAGATTTCGCAGGCGAAGCTCGACAAGCTCGTCGGCGA
ACGGCTGCAGGGGCGCTTCTCGCCCGCGCTGCTCGCGCGGATTCAGCTCGTCGCGTTCCGTCCGCTCGACGTCGACGCGC
TGACCGGCATCGCGACGCAGGCGCTCGACGAACTCGGCGAGCGCCTCGCGGAGAACGATCTGCAATGGCGCGCGGACGAA
GGCGTCGCGTCGTGGGTCGCGCACGCGGTGTCGCAGCATCCGGCGAACGGGCGCGCGGTGCGCGACCTGCTGCGCCAGCA
CGTGATGCCGGCCGTCGCGCGCGGCGTGCTCGCCGCGCGGGCCGAAGGGCGCGCGCTGAAGACCGTGCGGCTGTCCGCGC
ACGAGAAGCTGTCGCTCGTGTTCGACGAGGATGCGCTGGAGATCGGCGGCACCGATGCGGCCTCGCTCGGCGAGCAGGCG
GAGGCGGCGGCGCGCGAGGCTGCGGCGTCCGCTGCGGCGCACGGCGCACCCGAAGCGCATGACGCACCTGAGTCGCGTGA
CGTGCATGAAGCGAACGGCGACATGCGTGCCGAAGCGGACGACGAACGTCCGGCCGATGCGCATCATCCCGACGAAACGA
CCTCGGCGAACGCCGGCACGACGGGAGAACCGTCATGCGTCTGA