Detailed information of component protein


Summary

Component ID T6CP001081
Component protein type TssD
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (176 a.a.); Nucleotide sequence (528 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09720
Coordinate (Strand) 2304861..2305388 (-)
NCBI ID WP_009901013.1
RefSeq NC_007650
Uniprot ID Q2T406_BURTA, A0A7X3E616_BURTH
KEGG ID bte:BTH_II1899
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_HCP PF05638.14 3.2e-38 9..150
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-175 MANALVDYFLQIDGVEGESTDSQYPGLIQIQSWQWAEENSGRWGFGSGGGAGKVEMKDFEFRMVSNKASPKLFLMCATGEHIQNAKLICRKSGKGQQEFLTISFASGLVSSFRTLGNMPISQLGHASGEVDGVLPTDQIRINFAQIEFEYREQRNDGTMGAVIKAGYDLKQNAPI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9880 - -


  Protein sequence: 176 a.a.    

>T6CP001081 NC_007650:c2305388-2304861 [Burkholderia thailandensis E264] [TssD]
MANALVDYFLQIDGVEGESTDSQYPGLIQIQSWQWAEENSGRWGFGSGGGAGKVEMKDFEFRMVSNKASPKLFLMCATGE
HIQNAKLICRKSGKGQQEFLTISFASGLVSSFRTLGNMPISQLGHASGEVDGVLPTDQIRINFAQIEFEYREQRNDGTMG
AVIKAGYDLKQNAPI*

  Nucleotide sequence: 528 bp    

>T6CP001081 NC_007650:c2305388-2304861 [Burkholderia thailandensis E264] [TssD]
ATGGCAAATGCTTTGGTTGATTACTTCTTGCAGATCGACGGCGTCGAGGGTGAGTCGACCGATTCGCAATATCCCGGTCT
GATCCAGATTCAGAGCTGGCAGTGGGCGGAAGAGAACTCGGGCCGCTGGGGCTTCGGCAGCGGCGGCGGCGCGGGCAAGG
TCGAGATGAAGGACTTCGAGTTCCGCATGGTGTCGAACAAGGCTTCGCCGAAGCTGTTCCTGATGTGTGCGACGGGTGAG
CACATCCAGAACGCGAAGCTGATCTGCCGCAAGTCGGGCAAGGGCCAGCAGGAATTCCTGACGATCTCGTTCGCGAGCGG
CCTCGTCTCGTCGTTCCGCACGCTCGGCAACATGCCGATCTCGCAGCTCGGCCACGCTAGCGGCGAAGTCGACGGCGTGC
TGCCGACCGACCAGATCCGGATCAACTTCGCCCAGATCGAGTTCGAGTACCGCGAGCAGCGCAACGACGGCACGATGGGC
GCAGTGATCAAGGCCGGATACGACCTCAAGCAGAACGCCCCGATCTAA