Detailed information of regulator
Summary
| Regulator ID | REG0154  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | Quorum sensing (map02024) |
| Related T6SS | T6SS00114 (Type i3) |
| Strain | Burkholderia thailandensis E264 |
| Replicon | chromosome |
| Sequence | Protein sequence (155 a.a.); Nucleotide sequence (465 bp) |
| Description | bth_I0691(Zur) in chromosome I directly represses expression of T6SS4 in chromosome II by interaction of T6SS4 promoter. |
| Reference |
External database links
| Locus tag (Gene) | BTH_I0691 (Zur) |
| Coordinates (Strand) | 796074..796538 (-) |
| NCBI ID | 497578547 |
| RefSeq | NC_007651 |
| Uniprot ID | UPI00016A4226 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map02024 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| BTH_RS09735 (T6SS-4) Promotor,−541 to −1 relative to the ATG start codon | ↑ | ggtctatccacaaatcacaggcgttaggtaccgacttcgcaaaaccagattagcttgcgtcttgtcaaaatacccgaacttatttcttcgaatgtattagcgaccacataactcgatgccgaccatcagtcaaagcggcgtggggaattagattagtactagccggaaaaaggcaaacaaacgcccgcggcgtccgggaaaatattccacggaaaccgtttacgctgctgtttttttctcagtggtattaacccaccccttccgcccagcccgatccctgcgagtcggcatacgccggcttccgcaacggcaatcggtaagatgcactcccgctaaatcaaataccgcacgcgcataaatcgatgcgccgtcgcgatcgcggcggaaataacatccaagcaccgggtttatctgaagcgtcacaccttagtggccctcaacgacaacggcgtgacaatcgttcatatcgttacagtctacgctgtaaacttatagaacgctcgtaaatttggcaagcggaaataattttttcttacaaac Identification of a Zur binding site in the T6SS4 promoter region, indicated by red |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FUR | PF01475.21 | 7.2e-11 | 21..138 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-154 | MASTHSLAARLSRADAFAAEHGLAWTPLRRQVYERVLAAGRPTGAYDLLAELEPQRGRVPPTTVYRALEFLVEHGFIHRIESKNAFIACCEIGKPHEGQFLICEECGDTVEIPGGDLAKQLSSSPPAHGFEVHRQVVELTGLCGQCKTKHAHHG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9618 | - | - |
Protein sequence of regulator: 155 a.a. .
>REG0154 NC_007651:c796538-796074 [Burkholderia thailandensis E264]
MASTHSLAARLSRADAFAAEHGLAWTPLRRQVYERVLAAGRPTGAYDLLAELEPQRGRVPPTTVYRALEFLVEHGFIHRI
ESKNAFIACCEIGKPHEGQFLICEECGDTVEIPGGDLAKQLSSSPPAHGFEVHRQVVELTGLCGQCKTKHAHHG*
MASTHSLAARLSRADAFAAEHGLAWTPLRRQVYERVLAAGRPTGAYDLLAELEPQRGRVPPTTVYRALEFLVEHGFIHRI
ESKNAFIACCEIGKPHEGQFLICEECGDTVEIPGGDLAKQLSSSPPAHGFEVHRQVVELTGLCGQCKTKHAHHG*
Nucleotide sequence of regulator: 465 bp .
>REG0154 NC_007651:c796538-796074 [Burkholderia thailandensis E264]
ATGGCATCGACCCACTCCCTCGCCGCCCGCCTTTCCCGCGCCGACGCGTTCGCCGCCGAGCACGGCCTCGCGTGGACGCC
GCTGCGCCGCCAGGTCTACGAGCGTGTGCTCGCCGCCGGCCGGCCGACCGGCGCGTACGATCTGCTCGCCGAGCTCGAGC
CGCAGCGCGGCCGCGTGCCGCCGACGACCGTCTATCGCGCGCTCGAGTTTCTCGTCGAGCACGGCTTCATCCACCGGATC
GAATCGAAGAACGCGTTCATCGCGTGCTGCGAGATCGGCAAGCCGCACGAAGGGCAGTTCCTGATCTGCGAGGAATGCGG
CGACACCGTCGAGATTCCCGGCGGCGATCTCGCGAAGCAGCTGTCGTCGAGCCCGCCCGCGCACGGCTTCGAGGTGCATC
GGCAGGTCGTCGAGCTGACCGGCCTGTGCGGGCAGTGCAAGACGAAGCACGCGCATCACGGCTGA
ATGGCATCGACCCACTCCCTCGCCGCCCGCCTTTCCCGCGCCGACGCGTTCGCCGCCGAGCACGGCCTCGCGTGGACGCC
GCTGCGCCGCCAGGTCTACGAGCGTGTGCTCGCCGCCGGCCGGCCGACCGGCGCGTACGATCTGCTCGCCGAGCTCGAGC
CGCAGCGCGGCCGCGTGCCGCCGACGACCGTCTATCGCGCGCTCGAGTTTCTCGTCGAGCACGGCTTCATCCACCGGATC
GAATCGAAGAACGCGTTCATCGCGTGCTGCGAGATCGGCAAGCCGCACGAAGGGCAGTTCCTGATCTGCGAGGAATGCGG
CGACACCGTCGAGATTCCCGGCGGCGATCTCGCGAAGCAGCTGTCGTCGAGCCCGCCCGCGCACGGCTTCGAGGTGCATC
GGCAGGTCGTCGAGCTGACCGGCCTGTGCGGGCAGTGCAAGACGAAGCACGCGCATCACGGCTGA