Detailed information of component protein


Summary

Component ID T6CP009346
Component protein type TssJ
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (198 a.a.); Nucleotide sequence (594 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09665
Coordinate (Strand) 2288867..2289460 (-)
NCBI ID WP_009893760.1
RefSeq NC_007650
Uniprot ID A0A7X3J5Y3_BURTH
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 1.1e-11 35..152
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-197 VRRKMGPFKSFVAALALGVLAGCSAFSSSKPEEPRQLHVTLVGGSRLNAAPTGEARPIQTCVYVVTAADWLPTQGGDDSSCASRGQDSTVVADSRHVIAPNQVLQFSLNLPRSGDIWLVADADYARRPANYGPLRVRIEGRGLIHMAVWLDRDGIYNALLPGPVPVGGAIAASAIRTDEPPPERRTKTTYGIRRSRQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9961 pos: 22-23. LAG-CS. VRRKMGPFKSFVAALALGVLAG


  Protein sequence: 198 a.a.    

>T6CP009346 NC_007650:c2289460-2288867 [Burkholderia thailandensis E264] [TssJ]
VRRKMGPFKSFVAALALGVLAGCSAFSSSKPEEPRQLHVTLVGGSRLNAAPTGEARPIQTCVYVVTAADWLPTQGGDDSS
CASRGQDSTVVADSRHVIAPNQVLQFSLNLPRSGDIWLVADADYARRPANYGPLRVRIEGRGLIHMAVWLDRDGIYNALL
PGPVPVGGAIAASAIRTDEPPPERRTKTTYGIRRSRQ*

  Nucleotide sequence: 594 bp    

>T6CP009346 NC_007650:c2289460-2288867 [Burkholderia thailandensis E264] [TssJ]
GTGCGTCGCAAGATGGGCCCCTTCAAATCGTTCGTGGCGGCGCTCGCGCTCGGCGTGCTCGCGGGCTGCTCCGCGTTTTC
GTCATCGAAGCCGGAAGAGCCGCGGCAGTTGCACGTGACGCTCGTCGGCGGCAGCCGGCTGAACGCCGCGCCGACGGGCG
AGGCGCGTCCGATCCAGACGTGCGTGTATGTCGTGACGGCGGCGGACTGGCTGCCCACGCAAGGCGGCGACGATTCGTCG
TGCGCGTCGCGCGGGCAGGACAGCACGGTCGTCGCCGACTCGCGTCACGTGATCGCGCCGAACCAGGTGCTGCAGTTCTC
GCTGAACCTGCCGCGCTCCGGCGACATCTGGCTCGTCGCCGACGCCGACTACGCGCGCCGGCCGGCGAACTACGGGCCGC
TGCGCGTGCGCATCGAGGGCCGCGGATTGATTCACATGGCTGTCTGGCTTGACCGCGACGGCATCTACAACGCGTTGTTG
CCGGGGCCCGTGCCCGTCGGCGGCGCGATCGCCGCATCGGCGATCCGCACCGACGAGCCGCCGCCCGAGCGCAGAACGAA
AACCACCTATGGGATAAGGAGAAGCAGGCAATGA