Detailed information of component protein


Summary

Component ID T6CP001082
Component protein type TssC
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (502 a.a.); Nucleotide sequence (1506 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09725
Coordinate (Strand) 2305532..2307037 (-)
NCBI ID WP_009901014.1
RefSeq NC_007650
Uniprot ID UPI0000667AC1
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 3.1e-127 69..371
VipB_2 PF18945.2 1.4e-43 381..492
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-501 MQQLESSAEKVVVDQNNVNEDLKDILRRSFRPRTNEAAEAVQNAVETLLTYARRSRVVVREDVAQTIEQLVAELDKKISEQLTLVLHNKRFQSLEGAWRGLHYLVSNTDTSENLKIRYLNISKADLGKTLRRFKGVVWDQSPIFKMIYEQEYGQFGGEPFGCLIGDFYFDHSMQDVSILTEMSKISAAAHAPFIAAAAPGLLQMDDWSELSNPRDVSKIFTATEYAFWRRLRESNDSRYLALTLPRFLARVPYGPKTQPVEEFGFEEKVDPNRAEDFCWANSAYAMGANITRAFKTYGWCTKIRGVESGGAVEVLPKFVLPSQDREVDLHCPTEIAISDRREHELSESGLMPLVYRKNSDTAAFIGAKTVHRPAIYEDDDATANSNLSSRLPYIFATCRFAHYLKCIVRDKIGSFKSAEDTQRWLNDWLMNYVDGDPSISSEVTKSQRPLSAAEVVVDEIPENPGYYRAQFFLRPHFQLEGLTVSLRLVSKLPSTKQEVTT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9912 - -


  Protein sequence: 502 a.a.    

>T6CP001082 NC_007650:c2307037-2305532 [Burkholderia thailandensis E264] [TssC]
MQQLESSAEKVVVDQNNVNEDLKDILRRSFRPRTNEAAEAVQNAVETLLTYARRSRVVVREDVAQTIEQLVAELDKKISE
QLTLVLHNKRFQSLEGAWRGLHYLVSNTDTSENLKIRYLNISKADLGKTLRRFKGVVWDQSPIFKMIYEQEYGQFGGEPF
GCLIGDFYFDHSMQDVSILTEMSKISAAAHAPFIAAAAPGLLQMDDWSELSNPRDVSKIFTATEYAFWRRLRESNDSRYL
ALTLPRFLARVPYGPKTQPVEEFGFEEKVDPNRAEDFCWANSAYAMGANITRAFKTYGWCTKIRGVESGGAVEVLPKFVL
PSQDREVDLHCPTEIAISDRREHELSESGLMPLVYRKNSDTAAFIGAKTVHRPAIYEDDDATANSNLSSRLPYIFATCRF
AHYLKCIVRDKIGSFKSAEDTQRWLNDWLMNYVDGDPSISSEVTKSQRPLSAAEVVVDEIPENPGYYRAQFFLRPHFQLE
GLTVSLRLVSKLPSTKQEVTT*

  Nucleotide sequence: 1506 bp    

>T6CP001082 NC_007650:c2307037-2305532 [Burkholderia thailandensis E264] [TssC]
ATGCAGCAGCTTGAATCGTCTGCCGAGAAGGTGGTCGTCGATCAGAACAATGTCAACGAGGATCTGAAGGACATCCTGCG
CCGCTCGTTTCGGCCGCGCACCAACGAGGCGGCCGAGGCGGTGCAGAACGCCGTCGAGACCCTGCTCACGTATGCGCGGC
GCAGCCGCGTCGTGGTTCGCGAGGATGTCGCGCAGACGATCGAGCAGTTGGTTGCCGAGCTCGACAAGAAGATTTCCGAG
CAACTGACGCTCGTGCTGCACAACAAGCGCTTCCAGAGTCTCGAAGGCGCATGGCGCGGATTGCACTACCTCGTGTCGAA
CACCGACACGAGCGAGAACCTGAAGATCCGCTACCTGAACATCTCGAAAGCGGATCTCGGCAAGACGCTGCGGCGCTTCA
AGGGCGTCGTGTGGGACCAGAGTCCGATTTTCAAGATGATCTACGAGCAGGAGTACGGCCAGTTCGGCGGCGAGCCGTTC
GGCTGCCTGATCGGTGACTTCTACTTCGACCACAGCATGCAGGACGTGTCGATCCTCACCGAGATGTCGAAGATCTCGGC
GGCCGCGCACGCGCCGTTCATCGCGGCCGCGGCGCCGGGCCTGCTGCAGATGGACGACTGGAGCGAGCTGTCGAATCCGC
GCGACGTGTCGAAGATCTTTACCGCGACCGAATACGCGTTCTGGCGGCGCCTGCGCGAGAGCAACGACTCGCGCTACCTC
GCGCTCACGCTGCCGCGCTTTCTCGCGCGCGTGCCGTATGGCCCGAAGACGCAGCCCGTCGAAGAGTTCGGATTCGAGGA
GAAGGTCGATCCGAACCGCGCCGAGGACTTCTGCTGGGCGAACTCCGCCTATGCGATGGGCGCGAACATCACGCGCGCGT
TCAAGACCTACGGCTGGTGCACGAAGATTCGCGGCGTCGAGTCGGGCGGCGCGGTCGAGGTGCTGCCGAAGTTCGTGCTG
CCGTCGCAGGATCGCGAAGTCGATCTGCACTGCCCGACCGAAATCGCGATCTCGGACCGCCGCGAGCACGAGCTGTCGGA
ATCCGGCCTGATGCCGCTCGTGTATCGGAAGAACTCGGATACGGCTGCGTTCATCGGCGCGAAGACCGTGCACCGTCCGG
CAATCTACGAGGACGACGACGCGACCGCGAACTCGAATCTGTCGTCGCGGCTGCCGTACATCTTCGCGACGTGCCGCTTC
GCGCACTACCTGAAGTGCATCGTGCGCGACAAGATCGGTTCGTTCAAGTCGGCCGAGGACACGCAGCGTTGGCTCAACGA
CTGGCTGATGAACTACGTGGACGGCGACCCGAGCATCTCGAGCGAGGTGACGAAATCGCAGCGTCCGCTGTCGGCGGCGG
AAGTGGTCGTCGACGAGATTCCGGAGAACCCCGGCTACTATCGCGCGCAGTTTTTCCTGCGCCCCCATTTCCAACTGGAG
GGTTTGACCGTTTCTCTCCGGCTGGTTTCGAAGCTTCCGTCGACCAAGCAAGAGGTGACGACCTGA