Detailed information of component protein


Summary

Component ID T6CP001075
Component protein type TssI
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (763 a.a.); Nucleotide sequence (2289 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09690
Coordinate (Strand) 2293904..2296192 (-)
NCBI ID WP_009893768.1
RefSeq NC_007650
Uniprot ID Q2T412_BURTA
KEGG ID bte:BTH_II1893
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 3e-78 23..327
Phage_base_V PF04717.14 1.1e-13 380..449
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-762 MRLIELRSPLLDPDVVALSFVVHENLSQEPSYQLDLLSRDPNLDFDELLGSTLSADIDLGGGDIRTFNTHVFGGHDTGQMSGQYTYTLELRSWLSFLAENRNSRIFQDLSVPQIVEQVFQGHQRNGYRFELEGTYEPREYCVQFQETDLNFVKRLLEDEGIYFWVEHEPDRHVVVISDTQRFEDLPLPNETLEYLPDGEESRAIQGREGVQRLQRTRRIKASNVALRDFDYHAPSKQLDSDAQIEQQSLGGIPLEYYDYAAGYRDPEQGERLARLRLEAIQADAHALGGEANARALAVGRAFTLVGHPALSRNRRYYVTNSELTFIQDGPDSTSQGRNVAVKFRALADDRPYRPLLVTKRPRVPGIQSATVVGPEMSEVHTDKLGRIRVHFHWDRYKTTEADASCWIRVTQAWAGKGWGVLAMPRVGQEVIVVYVDGDLDRPLATGIVYNGENPTPYDLPKDIRYTGLVTRSIKRAGGIPNASQLTFDDQHGAERVMIHAERDLQQTVERNSSTSIAQDLNLSVNGTSTSVIGIKVSFTGISVSYTGLSVSFTGVSASFTGVSTSFTGVSTSFTGVSTSFTGVTTGFTGVSTSFVGVDTSFTGISTGFVGVSTSITGSKNSVTGVSNSMTGISSSWTDVSMSTTGQSQSITGVSLSYTGTSNSMTGTSTSVTGTSTSITGTSMSNTGSSTSITGTSMSTTGSSVGTTGSSMSATGSSVSTTGSSVSTTGSSMSVTGFSFSYIGASYSDVGIDLKKLGMQTKN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9903 - -


  Protein sequence: 763 a.a.    

>T6CP001075 NC_007650:c2296192-2293904 [Burkholderia thailandensis E264] [TssI]
MRLIELRSPLLDPDVVALSFVVHENLSQEPSYQLDLLSRDPNLDFDELLGSTLSADIDLGGGDIRTFNTHVFGGHDTGQM
SGQYTYTLELRSWLSFLAENRNSRIFQDLSVPQIVEQVFQGHQRNGYRFELEGTYEPREYCVQFQETDLNFVKRLLEDEG
IYFWVEHEPDRHVVVISDTQRFEDLPLPNETLEYLPDGEESRAIQGREGVQRLQRTRRIKASNVALRDFDYHAPSKQLDS
DAQIEQQSLGGIPLEYYDYAAGYRDPEQGERLARLRLEAIQADAHALGGEANARALAVGRAFTLVGHPALSRNRRYYVTN
SELTFIQDGPDSTSQGRNVAVKFRALADDRPYRPLLVTKRPRVPGIQSATVVGPEMSEVHTDKLGRIRVHFHWDRYKTTE
ADASCWIRVTQAWAGKGWGVLAMPRVGQEVIVVYVDGDLDRPLATGIVYNGENPTPYDLPKDIRYTGLVTRSIKRAGGIP
NASQLTFDDQHGAERVMIHAERDLQQTVERNSSTSIAQDLNLSVNGTSTSVIGIKVSFTGISVSYTGLSVSFTGVSASFT
GVSTSFTGVSTSFTGVSTSFTGVTTGFTGVSTSFVGVDTSFTGISTGFVGVSTSITGSKNSVTGVSNSMTGISSSWTDVS
MSTTGQSQSITGVSLSYTGTSNSMTGTSTSVTGTSTSITGTSMSNTGSSTSITGTSMSTTGSSVGTTGSSMSATGSSVST
TGSSVSTTGSSMSVTGFSFSYIGASYSDVGIDLKKLGMQTKN*

  Nucleotide sequence: 2289 bp    

>T6CP001075 NC_007650:c2296192-2293904 [Burkholderia thailandensis E264] [TssI]
ATGCGTCTGATCGAACTACGCAGTCCGCTCCTGGACCCGGACGTCGTTGCGCTGAGCTTCGTGGTGCACGAGAACCTGTC
GCAGGAACCGTCGTATCAGCTCGACTTGCTGAGCCGCGATCCGAATCTGGACTTCGACGAACTGCTCGGCTCGACGCTGT
CGGCCGACATCGATCTGGGCGGCGGCGACATCCGGACGTTCAACACGCACGTGTTCGGCGGCCACGACACGGGGCAGATG
AGCGGGCAGTACACGTACACGCTGGAGCTGCGGAGCTGGCTGTCGTTTCTCGCGGAGAACCGCAACAGCCGGATCTTCCA
GGACTTGAGCGTGCCGCAGATCGTCGAGCAGGTGTTCCAGGGCCATCAGCGCAACGGCTACCGGTTCGAACTGGAAGGCA
CGTACGAGCCGCGCGAGTACTGCGTGCAGTTCCAGGAAACGGATCTGAACTTCGTGAAGCGGTTGCTGGAGGACGAAGGG
ATCTATTTCTGGGTCGAGCACGAGCCGGATCGGCATGTCGTGGTGATTTCGGACACGCAGCGCTTCGAGGATCTGCCGCT
GCCGAACGAAACGCTCGAGTATTTGCCGGACGGTGAGGAATCGCGGGCGATCCAGGGGCGCGAAGGCGTGCAGCGGCTGC
AGCGCACGCGCCGGATCAAGGCGAGCAACGTCGCGCTGCGCGACTTCGACTATCACGCGCCGTCGAAGCAACTGGACAGC
GACGCGCAGATCGAGCAGCAGAGTCTCGGCGGCATTCCGCTCGAGTACTACGACTACGCGGCCGGCTACCGCGATCCCGA
GCAAGGCGAGCGCCTGGCGCGGCTGCGGCTTGAAGCGATTCAGGCCGATGCACACGCGCTCGGGGGCGAGGCGAACGCAC
GCGCGCTCGCGGTGGGTCGCGCGTTCACGCTGGTTGGGCATCCGGCGCTGAGCCGCAATCGTCGGTACTACGTGACGAAC
AGCGAGCTGACGTTCATTCAGGACGGGCCGGACAGCACGTCGCAGGGGCGCAACGTCGCGGTGAAGTTCCGCGCGCTCGC
CGACGATCGGCCGTATCGGCCGCTGCTCGTCACCAAGCGGCCGCGCGTGCCGGGCATCCAGAGCGCGACGGTGGTGGGGC
CAGAGATGTCGGAGGTGCATACCGACAAGCTCGGGCGCATCCGCGTGCACTTCCACTGGGACCGCTACAAGACGACCGAA
GCGGACGCCTCGTGCTGGATTCGCGTGACGCAGGCGTGGGCCGGCAAGGGCTGGGGTGTGCTCGCGATGCCGCGGGTCGG
GCAGGAAGTGATCGTCGTGTATGTCGACGGCGATCTCGATCGCCCGCTTGCGACGGGCATCGTCTACAACGGCGAAAACC
CGACGCCTTACGACCTGCCGAAGGACATCCGCTACACGGGCCTCGTCACGCGCTCGATCAAGCGCGCGGGCGGCATTCCG
AATGCGAGCCAGTTGACGTTCGACGATCAGCACGGCGCGGAGCGCGTGATGATCCACGCGGAGCGCGACTTGCAGCAGAC
GGTCGAGCGCAACAGCTCCACGTCGATCGCGCAGGACCTGAATCTGTCGGTGAACGGTACGTCGACGTCGGTCATCGGCA
TCAAGGTCAGCTTCACCGGGATCTCGGTGTCGTACACAGGGCTGTCGGTGAGCTTCACGGGCGTATCGGCGAGCTTCACC
GGCGTGAGCACATCGTTTACCGGCGTGAGCACGAGCTTCACCGGCGTGTCGACGTCGTTTACCGGCGTGACCACGGGCTT
CACCGGCGTGTCGACTTCGTTCGTCGGCGTCGATACGAGCTTCACCGGCATCAGCACGGGATTCGTCGGTGTGTCAACCA
GCATCACGGGCTCCAAGAATTCCGTGACGGGCGTGTCGAACAGCATGACGGGCATCTCGTCGTCGTGGACCGACGTCAGC
ATGTCGACGACCGGGCAGTCGCAGAGCATCACGGGCGTATCGCTGTCGTACACGGGCACGTCGAACAGCATGACGGGCAC
GAGCACGTCGGTGACGGGCACGTCGACGAGCATCACGGGCACGTCGATGTCGAACACGGGCAGCTCGACGAGCATCACGG
GCACGTCGATGTCGACGACGGGCAGCTCGGTGGGCACGACGGGATCGAGCATGTCGGCCACCGGCAGCTCGGTGAGCACG
ACGGGTTCGAGCGTATCGACGACGGGCAGCAGCATGTCGGTGACGGGATTCAGCTTCTCGTACATCGGCGCGAGCTACTC
GGACGTGGGCATTGATCTGAAGAAGCTCGGGATGCAGACGAAGAACTGA