Detailed information of component protein


Summary

Component ID T6CP001079
Component protein type TssF
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (621 a.a.); Nucleotide sequence (1863 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09710
Coordinate (Strand) 2302475..2304337 (-)
NCBI ID WP_009901011.1
RefSeq NC_007650
Uniprot ID Q2T408_BURTA, A0A7X3DVZ9_BURTH
KEGG ID bte:BTH_II1897
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 7.8e-111 2..606
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-620 MNFLDHYNDELRQLRDAGARFAKEHPQVASALGLHPDAVTDPFVERLLEGVAYLSARVQKRLDRECAEFAQQALGRICPLYMASTPAISTFAFHPDLGSPDAFRGNTLPRGSLVAAHLPGRKLPVMFSTARDVTLLPLRLASVECSRSITGLPSLLSQRLASSHAVLRFRFEVEGGASIAELAREDEGFKPLHLSLAGDLPRAYALHRAMLADTTAWYALVSTNRGDEVLTLPMSGIRLSGVDDAEALLPEEFGGLPGLRLLREYFAQPTRLLGVYVDALAAIAAKAPSARAFELFFALRDAPGDLVGDVDASQFRLFATPAINLYSKRFDPVPYDANQPEQWIPVDRMRPAAHHLWALTEVFVCETNGRAHRARSVLETAGYEGHEGSIRYGMRREDALLVDGVRRDRFDPLASHDLIAVSMVDDTLDPDDVATITGRALVADRDWRPTGLLDADLQLLDPTAVKRIECLWPASAPRGKPSVDACWEAVSHVGRNPLALHASQQQDVTARIVEQLLLAIDKDDALDRQRLESLRSVLLRSRFVAAGRATPTALVRATQVEIDIAESLHADRGGWLFGRLLAQALAEAATLNDGIEIVVRLDGEAASTHTNVAARDGRLA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9891 - -


  Protein sequence: 621 a.a.    

>T6CP001079 NC_007650:c2304337-2302475 [Burkholderia thailandensis E264] [TssF]
MNFLDHYNDELRQLRDAGARFAKEHPQVASALGLHPDAVTDPFVERLLEGVAYLSARVQKRLDRECAEFAQQALGRICPL
YMASTPAISTFAFHPDLGSPDAFRGNTLPRGSLVAAHLPGRKLPVMFSTARDVTLLPLRLASVECSRSITGLPSLLSQRL
ASSHAVLRFRFEVEGGASIAELAREDEGFKPLHLSLAGDLPRAYALHRAMLADTTAWYALVSTNRGDEVLTLPMSGIRLS
GVDDAEALLPEEFGGLPGLRLLREYFAQPTRLLGVYVDALAAIAAKAPSARAFELFFALRDAPGDLVGDVDASQFRLFAT
PAINLYSKRFDPVPYDANQPEQWIPVDRMRPAAHHLWALTEVFVCETNGRAHRARSVLETAGYEGHEGSIRYGMRREDAL
LVDGVRRDRFDPLASHDLIAVSMVDDTLDPDDVATITGRALVADRDWRPTGLLDADLQLLDPTAVKRIECLWPASAPRGK
PSVDACWEAVSHVGRNPLALHASQQQDVTARIVEQLLLAIDKDDALDRQRLESLRSVLLRSRFVAAGRATPTALVRATQV
EIDIAESLHADRGGWLFGRLLAQALAEAATLNDGIEIVVRLDGEAASTHTNVAARDGRLA*

  Nucleotide sequence: 1863 bp    

>T6CP001079 NC_007650:c2304337-2302475 [Burkholderia thailandensis E264] [TssF]
ATGAATTTCCTCGATCACTACAACGACGAATTGCGCCAGCTGCGCGATGCCGGCGCGCGCTTTGCGAAAGAGCACCCGCA
GGTCGCGTCGGCGCTCGGGCTGCATCCCGATGCGGTCACCGATCCGTTCGTCGAGCGGTTGCTCGAGGGCGTCGCGTATC
TGTCGGCGCGCGTGCAGAAGCGGCTCGATCGCGAGTGCGCCGAGTTCGCGCAGCAGGCGCTCGGGCGCATCTGCCCGCTC
TACATGGCGTCGACGCCCGCGATCTCGACGTTCGCGTTCCATCCGGATCTCGGCTCGCCCGACGCGTTTCGCGGCAATAC
GCTGCCGCGCGGCTCGCTCGTCGCCGCGCATCTGCCGGGCCGCAAGCTGCCGGTGATGTTCTCGACCGCGCGCGACGTCA
CGCTGCTGCCGCTGCGTCTGGCGTCGGTCGAGTGCAGCCGCAGCATCACCGGGCTGCCGTCGCTGCTCTCGCAGCGGCTG
GCATCGTCGCATGCGGTGCTGCGCTTCAGGTTCGAGGTCGAAGGCGGCGCGTCGATCGCGGAGCTCGCGCGCGAGGACGA
GGGTTTCAAGCCGCTGCATCTGAGTCTCGCGGGCGATCTGCCGCGCGCCTATGCGCTGCATCGCGCGATGCTCGCCGATA
CGACCGCGTGGTATGCGCTCGTGTCGACCAATCGCGGCGACGAGGTGCTGACGTTGCCGATGTCGGGCATCCGGCTGTCC
GGCGTCGACGATGCGGAGGCGCTGCTGCCGGAGGAGTTCGGCGGCTTGCCCGGCCTGCGGCTGCTGCGCGAGTACTTCGC
GCAGCCGACGCGTCTGCTCGGCGTCTACGTCGACGCGCTGGCGGCGATCGCCGCCAAGGCGCCGTCGGCGCGGGCGTTCG
AGCTGTTCTTCGCATTACGCGACGCACCGGGCGATCTGGTCGGCGACGTTGACGCGAGCCAGTTCCGCCTGTTCGCGACG
CCGGCGATCAATCTGTATTCCAAACGTTTCGATCCGGTGCCCTACGACGCGAATCAGCCCGAGCAGTGGATTCCCGTCGA
CCGGATGCGGCCCGCCGCGCATCACCTGTGGGCGCTGACCGAGGTGTTCGTGTGCGAGACCAACGGTCGAGCGCATCGCG
CGCGCTCGGTGCTCGAGACGGCGGGCTATGAGGGCCACGAGGGCTCGATCCGCTACGGCATGCGGCGCGAGGATGCGCTG
CTCGTCGACGGCGTGCGGCGTGATCGGTTCGATCCGCTCGCGAGCCACGACCTGATCGCCGTCTCGATGGTCGACGACAC
GCTCGACCCGGACGACGTCGCGACGATCACGGGCCGCGCGCTCGTGGCCGATCGCGACTGGCGTCCGACCGGGCTGCTCG
ACGCCGACCTGCAACTGCTCGATCCGACCGCCGTCAAGCGTATCGAGTGCCTGTGGCCGGCGAGCGCGCCGCGCGGCAAG
CCGAGCGTCGACGCGTGCTGGGAAGCCGTGTCGCACGTCGGCCGCAATCCGCTCGCGCTGCACGCGTCGCAACAGCAGGA
CGTGACGGCGCGCATCGTCGAGCAACTGCTGCTCGCGATCGACAAGGACGATGCGCTCGACCGGCAACGGCTCGAGAGCC
TGCGCTCGGTCCTGCTGCGCTCGCGCTTCGTCGCGGCGGGCCGGGCGACGCCCACCGCGCTCGTGCGCGCGACGCAAGTC
GAGATCGATATCGCCGAATCGCTGCATGCGGATCGCGGCGGCTGGCTGTTCGGGCGGCTGCTCGCGCAGGCGCTCGCCGA
GGCCGCGACGCTCAATGACGGCATCGAGATCGTCGTGCGCCTCGACGGCGAGGCGGCGAGCACGCACACGAACGTCGCGG
CGCGCGACGGGAGGCTCGCATGA