Detailed information of component protein


Summary

Component ID T6CP001076
Component protein type TssI
T6SS ID (Type) T6SS00114 experimental (Type i3)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (764 a.a.); Nucleotide sequence (2292 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Si M, Wang Y, Zhang B, et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition. Cell Rep. 2017 Jul 25;20(4):949-959. doi: 10.1016/j.celrep.2017.06.081. PMID: 28746878
[4] Si M, Zhao C, Burkinshaw B, et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2233-E2242. doi: 10.1073/pnas.1614902114. Epub 2017 Feb 27. PMID: 28242693
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS09695
Coordinate (Strand) 2296361..2298652 (-)
NCBI ID WP_011401423.1
RefSeq NC_007650
Uniprot ID Q2T411_BURTA, A0A7X3E627_BURTH
KEGG ID bte:BTH_II1894
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 8e-78 23..328
Phage_base_V PF04717.14 3.1e-14 381..450
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-763 MRLIELRSPLLDPDAVALSFVVHENLSQEPSYQLDLLSHDPDLDFDALLGSTLSADIDLGGGDIRTFNTHVFGGHDTGQMSGQYTYTLELRSWLSFLAENRNSRIFQNMSVPQIVEQVFQGHQRNGYRFELEGTYEPREYCVQFQETDLNFVKRLLEDEGIYFWVEHEPDRHVVVISDTQRFEDLPLPNETLEYLPDGEESRAIQGREGVQRLQRTRRIKASNVALRDFDYHAPSNKLDSDAQLVSPPNLEGIPLEYYDYAAGYREPEQGERLARLRLEAIQADSHMLVGEANARALATGRAFTLIGHPALGRNRRYYVTNSELTFIQDGPDSTSQGRNVAVKFRALADDQPYRPLLTTPRPEVPGIQSATVVGPEMSEVHTDKLGRIRVHFHWDRYKTTEADASCWIRVSQAWAGKGWGVIAMPRVGQEVLITYVDGDLDRPLVTGIVYNGENPTPYDLPKDIRYTGLVSRSIKRAGGYQNASQITFDDQRGAERVMIHAERDMQQTVERNSSTSIAQDLNLSVKGTATSVVGISVSFTGISVSYTGLSVSFTGVSASFTGVSTSFTGVSTSFTGVSTSFTGVSTSFIGVSTSFTGVDTGFKGVSTAMIGVSTSVVGSSNSVTGVSNSMTGISSSWTDVSMSTTGQSQSMTGVSLSYTGTSNSMTGTSTSVTGTSTSITGTSMSNTGSSTSITGTSMSTTGSSTSITGSSVSTTGSSVSTTGSSVSTTGSSVSTTGFSFSYTGASYSDVGIDLKKVGMQVKS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9900 - -


  Protein sequence: 764 a.a.    

>T6CP001076 NC_007650:c2298652-2296361 [Burkholderia thailandensis E264] [TssI]
MRLIELRSPLLDPDAVALSFVVHENLSQEPSYQLDLLSHDPDLDFDALLGSTLSADIDLGGGDIRTFNTHVFGGHDTGQM
SGQYTYTLELRSWLSFLAENRNSRIFQNMSVPQIVEQVFQGHQRNGYRFELEGTYEPREYCVQFQETDLNFVKRLLEDEG
IYFWVEHEPDRHVVVISDTQRFEDLPLPNETLEYLPDGEESRAIQGREGVQRLQRTRRIKASNVALRDFDYHAPSNKLDS
DAQLVSPPNLEGIPLEYYDYAAGYREPEQGERLARLRLEAIQADSHMLVGEANARALATGRAFTLIGHPALGRNRRYYVT
NSELTFIQDGPDSTSQGRNVAVKFRALADDQPYRPLLTTPRPEVPGIQSATVVGPEMSEVHTDKLGRIRVHFHWDRYKTT
EADASCWIRVSQAWAGKGWGVIAMPRVGQEVLITYVDGDLDRPLVTGIVYNGENPTPYDLPKDIRYTGLVSRSIKRAGGY
QNASQITFDDQRGAERVMIHAERDMQQTVERNSSTSIAQDLNLSVKGTATSVVGISVSFTGISVSYTGLSVSFTGVSASF
TGVSTSFTGVSTSFTGVSTSFTGVSTSFIGVSTSFTGVDTGFKGVSTAMIGVSTSVVGSSNSVTGVSNSMTGISSSWTDV
SMSTTGQSQSMTGVSLSYTGTSNSMTGTSTSVTGTSTSITGTSMSNTGSSTSITGTSMSTTGSSTSITGSSVSTTGSSVS
TTGSSVSTTGSSVSTTGFSFSYTGASYSDVGIDLKKVGMQVKS*

  Nucleotide sequence: 2292 bp    

>T6CP001076 NC_007650:c2298652-2296361 [Burkholderia thailandensis E264] [TssI]
ATGCGTCTGATCGAACTGCGTAGTCCCCTGCTGGACCCGGATGCTGTGGCGCTGAGCTTCGTGGTGCACGAGAACCTGTC
GCAGGAACCGTCGTATCAGCTCGACTTGCTGAGTCACGACCCGGATCTGGACTTCGACGCGCTGCTCGGCTCGACACTGT
CGGCCGACATCGATCTGGGCGGCGGCGACATCCGGACGTTCAACACGCACGTGTTCGGCGGCCACGACACGGGGCAGATG
AGCGGGCAGTACACGTACACGCTGGAGCTGCGGAGCTGGCTGTCGTTTCTCGCGGAGAACCGCAACAGCCGGATCTTCCA
GAACATGAGCGTGCCGCAGATCGTCGAGCAGGTGTTCCAGGGCCATCAGCGCAACGGCTACCGGTTCGAACTGGAAGGCA
CGTACGAGCCGCGCGAGTACTGCGTGCAGTTCCAGGAAACGGATCTGAACTTCGTGAAGCGATTGCTGGAGGACGAAGGG
ATCTATTTCTGGGTCGAGCACGAGCCGGATCGGCATGTCGTGGTGATTTCGGACACGCAGCGCTTCGAGGATCTGCCGCT
GCCGAACGAAACGCTCGAGTATTTGCCGGACGGCGAGGAATCGCGGGCGATCCAGGGGCGCGAAGGCGTGCAGCGGCTGC
AGCGCACGCGCCGGATCAAGGCAAGCAACGTCGCGCTGCGCGACTTCGACTATCACGCGCCGTCGAACAAGCTCGACAGC
GACGCACAGCTGGTGTCGCCGCCGAATCTCGAAGGGATTCCGCTCGAGTACTACGACTATGCGGCCGGCTACCGCGAGCC
GGAGCAAGGGGAGCGTCTCGCGCGGCTGCGGCTCGAAGCGATCCAGGCGGATTCGCACATGCTCGTCGGCGAGGCGAACG
CGCGCGCGCTGGCGACGGGCCGGGCGTTCACGCTGATCGGGCATCCGGCGCTGGGCCGCAATCGTCGGTACTACGTGACG
AACAGCGAGCTGACGTTCATTCAGGACGGGCCGGACAGCACGTCGCAGGGGCGCAACGTCGCGGTGAAGTTCCGCGCGCT
CGCCGACGATCAGCCGTATCGGCCGCTCTTGACGACGCCGCGCCCCGAGGTGCCGGGCATCCAGAGCGCGACGGTGGTGG
GGCCGGAGATGTCGGAGGTGCATACCGACAAGCTCGGGCGCATCCGCGTGCACTTCCACTGGGACCGCTACAAGACGACC
GAAGCGGACGCCTCGTGCTGGATTCGCGTGTCGCAGGCGTGGGCCGGCAAGGGCTGGGGCGTGATCGCGATGCCGCGGGT
CGGGCAGGAAGTGCTGATCACGTACGTCGACGGCGATCTCGACCGTCCGCTCGTGACGGGCATCGTCTACAACGGCGAAA
ACCCGACGCCTTACGATTTGCCGAAAGACATCCGCTACACGGGTCTCGTTTCACGTTCGATCAAGCGCGCCGGCGGCTAC
CAGAACGCAAGCCAGATCACGTTCGACGATCAGCGCGGCGCGGAGCGCGTGATGATCCACGCGGAGCGCGACATGCAGCA
GACGGTCGAGCGCAACAGCTCCACGTCGATCGCGCAGGACTTGAATCTGTCGGTGAAGGGCACGGCGACGTCGGTCGTCG
GGATCTCGGTCAGCTTCACGGGCATCTCGGTGTCGTACACGGGGCTGTCGGTGAGCTTCACGGGCGTATCGGCGAGTTTC
ACCGGCGTGAGCACATCGTTTACCGGCGTGAGCACGAGTTTCACCGGCGTGTCGACGTCGTTTACCGGCGTGAGCACGAG
CTTCATCGGCGTGTCGACGTCGTTTACCGGTGTCGATACAGGCTTCAAGGGCGTGTCGACGGCGATGATCGGTGTCTCGA
CCAGCGTCGTGGGTTCCAGCAATTCCGTGACGGGCGTGTCGAACAGCATGACGGGCATCTCGTCGTCGTGGACCGACGTC
AGCATGTCGACGACCGGGCAGTCGCAGAGCATGACCGGCGTATCGCTGTCGTACACGGGCACGTCGAACAGCATGACGGG
CACGAGCACGTCGGTGACGGGCACGTCGACGAGCATCACGGGCACGTCGATGTCGAACACGGGCAGCTCGACGAGCATCA
CGGGCACGTCGATGTCGACGACGGGCAGCTCGACGAGCATCACCGGCTCCAGCGTGTCGACGACCGGCAGCTCGGTGAGC
ACGACGGGTTCGAGCGTATCGACGACGGGCAGCAGCGTGTCGACGACGGGATTCAGCTTCTCGTACACCGGGGCGAGCTA
CTCGGACGTGGGCATCGACCTGAAGAAGGTCGGCATGCAAGTGAAGAGCTGA