Detailed information of effector
Summary
| Effector ID | EFF01825  |
| Effector type | Tie |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00114 (Type i3) |
| Strain | Burkholderia thailandensis E264 |
| Replicon | chromosome |
| Sequence | Protein sequence (120 a.a.); Nucleotide sequence (360 bp) |
| Description | T6SS4 secretes the zinc-binding effector TseZ for acquisition of Zn2+ under oxidative stress conditions and Interacts with the TonB-Dependent Heme Receptor HmuR |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BTH_II1884 (TseZ) |
| Coordinates (Strand) | 2281790..2282149 (-) |
| NCBI ID | 497579572 |
| RefSeq | NC_007650 |
| Uniprot ID | Q2T421_BURTA, A0A135HB79_BURTH |
| KEGG ID | btha:DR62_4340, bte:BTH_II1884 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF6277 | PF19793.1 | 1.9e-53 | 1..117 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-119 | MIDPKEIFAACNNAHQYGQDASGSMAKPFMDSIPNLSTASSSNASNLIGNVQEVGSKMLEHMSSIKSAVDKQVMIHQDNQQRQKTYDFNVDMRDLRPTNAVGFPEEMPSIFFAPFRTGK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9613 | - | - |
Protein sequence: 120 a.a. .
>EFF01825 NC_007650:c2282149-2281790 Type VI Metal Ion acquisition Effector (Tie) [Burkholderia thailandensis E264]
MIDPKEIFAACNNAHQYGQDASGSMAKPFMDSIPNLSTASSSNASNLIGNVQEVGSKMLEHMSSIKSAVDKQVMIHQDNQ
QRQKTYDFNVDMRDLRPTNAVGFPEEMPSIFFAPFRTGK*
MIDPKEIFAACNNAHQYGQDASGSMAKPFMDSIPNLSTASSSNASNLIGNVQEVGSKMLEHMSSIKSAVDKQVMIHQDNQ
QRQKTYDFNVDMRDLRPTNAVGFPEEMPSIFFAPFRTGK*
Nucleotide sequence: 360 bp .
>EFF01825 NC_007650:c2282149-2281790 Type VI Metal Ion acquisition Effector (Tie) [Burkholderia thailandensis E264]
ATGATTGATCCGAAGGAAATCTTCGCCGCATGCAACAACGCGCACCAGTACGGGCAGGACGCGAGCGGCTCGATGGCCAA
GCCGTTCATGGATTCGATCCCGAATCTGTCGACGGCGTCGTCGAGCAACGCGAGCAACCTGATCGGCAACGTGCAGGAGG
TCGGCTCGAAGATGCTCGAGCACATGTCGTCGATCAAGTCGGCGGTCGACAAGCAAGTGATGATCCATCAGGACAACCAG
CAGCGTCAGAAGACCTACGACTTCAACGTCGACATGCGGGACCTGCGCCCGACCAACGCGGTCGGTTTCCCGGAGGAGAT
GCCGTCGATCTTCTTCGCGCCGTTCAGGACCGGCAAGTGA
ATGATTGATCCGAAGGAAATCTTCGCCGCATGCAACAACGCGCACCAGTACGGGCAGGACGCGAGCGGCTCGATGGCCAA
GCCGTTCATGGATTCGATCCCGAATCTGTCGACGGCGTCGTCGAGCAACGCGAGCAACCTGATCGGCAACGTGCAGGAGG
TCGGCTCGAAGATGCTCGAGCACATGTCGTCGATCAAGTCGGCGGTCGACAAGCAAGTGATGATCCATCAGGACAACCAG
CAGCGTCAGAAGACCTACGACTTCAACGTCGACATGCGGGACCTGCGCCCGACCAACGCGGTCGGTTTCCCGGAGGAGAT
GCCGTCGATCTTCTTCGCGCCGTTCAGGACCGGCAAGTGA