Detailed information of effector
Summary
| Effector ID | EFF01464  |
| Effector type | Tie |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00114 (Type i3) |
| Strain | Burkholderia thailandensis E264 |
| Replicon | chromosome |
| Sequence | Protein sequence (142 a.a.); Nucleotide sequence (426 bp) |
| Description | Mn2+-binding effector TseM is required for T6SS-4-Cdependent Mn2+ acquisition under oxidative stress. TseM interacts with a Mn2+-Specific TonB-Dependent Outer Membrane Transporter. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BTH_II1883 (TseM) |
| Coordinates (Strand) | 2281342..2281767 (-) |
| NCBI ID | 83718045 |
| RefSeq | NC_007650 |
| Uniprot ID | UPI0000667A01 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-141 | MRRRQRASRAGVVARRATPGDAQANSGRAPARPFQSFVSNGAMLMNKQIVAGIVAGLVSMSSHAQLGQLFQSVKEQVTQAATSQVNQGVRSATDEAVQATSSRTRKAINSVRSPSSAAAATSTSPSAAEETNDATLSEARK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.6197 | - | - |
Protein sequence: 142 a.a. .
>EFF01464 NC_007650:c2281767-2281342 Type VI Metal Ion acquisition Effector (Tie) [Burkholderia thailandensis E264]
MRRRQRASRAGVVARRATPGDAQANSGRAPARPFQSFVSNGAMLMNKQIVAGIVAGLVSMSSHAQLGQLFQSVKEQVTQA
ATSQVNQGVRSATDEAVQATSSRTRKAINSVRSPSSAAAATSTSPSAAEETNDATLSEARK*
MRRRQRASRAGVVARRATPGDAQANSGRAPARPFQSFVSNGAMLMNKQIVAGIVAGLVSMSSHAQLGQLFQSVKEQVTQA
ATSQVNQGVRSATDEAVQATSSRTRKAINSVRSPSSAAAATSTSPSAAEETNDATLSEARK*
Nucleotide sequence: 426 bp .
>EFF01464 NC_007650:c2281767-2281342 Type VI Metal Ion acquisition Effector (Tie) [Burkholderia thailandensis E264]
TTGCGTCGGCGTCAGCGCGCTTCGCGCGCGGGCGTCGTCGCGCGCCGCGCGACGCCTGGCGATGCACAGGCGAATTCCGG
GCGCGCGCCGGCGCGCCCGTTTCAATCTTTTGTGAGCAATGGAGCAATGCTGATGAACAAGCAAATCGTGGCAGGGATCG
TGGCGGGGCTCGTGTCGATGAGCAGCCATGCGCAACTGGGGCAGTTGTTCCAGTCGGTGAAGGAGCAGGTGACGCAGGCG
GCGACGTCGCAGGTGAACCAGGGCGTGCGCTCGGCGACCGACGAGGCGGTGCAGGCCACCTCGTCGCGCACGCGCAAGGC
GATCAACTCGGTGCGCTCGCCTTCGTCGGCCGCCGCGGCTACGTCGACTTCGCCTTCGGCGGCCGAGGAGACGAATGACG
CGACGCTGAGCGAGGCGCGCAAGTAA
TTGCGTCGGCGTCAGCGCGCTTCGCGCGCGGGCGTCGTCGCGCGCCGCGCGACGCCTGGCGATGCACAGGCGAATTCCGG
GCGCGCGCCGGCGCGCCCGTTTCAATCTTTTGTGAGCAATGGAGCAATGCTGATGAACAAGCAAATCGTGGCAGGGATCG
TGGCGGGGCTCGTGTCGATGAGCAGCCATGCGCAACTGGGGCAGTTGTTCCAGTCGGTGAAGGAGCAGGTGACGCAGGCG
GCGACGTCGCAGGTGAACCAGGGCGTGCGCTCGGCGACCGACGAGGCGGTGCAGGCCACCTCGTCGCGCACGCGCAAGGC
GATCAACTCGGTGCGCTCGCCTTCGTCGGCCGCCGCGGCTACGTCGACTTCGCCTTCGGCGGCCGAGGAGACGAATGACG
CGACGCTGAGCGAGGCGCGCAAGTAA