Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00026  |
| T6SS type | Type i4b |
| Strain | Ralstonia solanacearum GMI1000 |
| Replicon (RefSeq) | plasmid pGMI1000MP (NC_003296) |
| Location | 926494..971950 |
| Function | anti-eukaryotic; effector translocation |
| Comment | TssB of R. solanacearum was required for secretion of Hcp, the hemolysin coregulated protein and a hallmark of T6S pathway. Deletion of tssB in R. solanacearum GMI1000 strain resulted in defect of biofilm formation, and the expression of the flagella operon is decreased, leading to decreased motility. More importantly, tssB mutant strain had significantly attenuated its virulence on tomato plants. |
| References | [1] Zhang L et al (2014) TssB is essential for virulence and required for Type VI secretion system in Ralstonia solanacearum. Microb Pathog. 74C:1-7. [PudMed:24972114] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | PAAR | TagL | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| RS_RS20630 | 922097..923044 | - | 948 | WP_011004031.1 | hypothetical protein | ||
| RS_RS20635 | 923063..923980 | - | 918 | WP_011004032.1 | hypothetical protein | ||
| RS_RS26215 | 924024..925082 | - | 1059 | WP_080511107.1 | hypothetical protein | ||
| RS_RS26960 | 925086..925349 | + | 264 | WP_135006091.1 | hypothetical protein | ||
| RS_RS26220 | 925361..925795 | - | 435 | WP_011004034.1 | hypothetical protein | ||
| RS_RS26225 | 925792..926493 | - | 702 | WP_011004035.1 | hypothetical protein | ||
| RS_RS20645 | 926731..929190 | - | 2460 | pseudogene | type VI secretion system tip protein VgrG | ||
| T6CP000200 | TssL | RS_RS20650 (icmH) | 929249..930040 | - | 792 | WP_011004037.1 | type IVB secretion system protein IcmH/DotU |
| T6CP000201 | TssK | RS_RS20655 (tssK) | 930037..931383 | - | 1347 | WP_011004038.1 | type VI secretion system baseplate subunit TssK |
| T6CP000202 | TssJ | RS_RS20660 (tssJ) | 931427..932101 | - | 675 | WP_028854260.1 | type VI secretion system lipoprotein TssJ |
| RS_RS20665 | 932376..933023 | + | 648 | WP_011004040.1 | hypothetical protein | ||
| T6CP000203 | TssB | RS_RS20670 (tssB) | 933060..933572 | + | 513 | WP_011004041.1 | type VI secretion system contractile sheath small subunit |
| T6CP000204 | TssC | RS_RS20675 (tssC) | 933565..935055 | + | 1491 | WP_011004042.1 | type VI secretion system contractile sheath large subunit |
| T6CP000205 | TssD | RS_RS20680 | 935144..935647 | + | 504 | WP_011004043.1 | type VI secretion system tube protein Hcp |
| T6CP000206 | TssE | RS_RS20685 (tssE) | 935708..936181 | + | 474 | WP_011004044.1 | type VI secretion system baseplate subunit TssE |
| T6CP000207 | TssF | RS_RS20690 (tssF) | 936244..938094 | + | 1851 | WP_011004045.1 | type VI secretion system baseplate subunit TssF |
| T6CP000208 | TssG | RS_RS20695 (tssG) | 938058..939149 | + | 1092 | WP_011004046.1 | type VI secretion system baseplate subunit TssG |
| T6CP000209 | TssH | RS_RS20700 (tssH) | 939182..941899 | + | 2718 | WP_011004047.1 | type VI secretion system ATPase TssH |
| T6CP000210 | TssI | RS_RS20705 | 941920..944676 | + | 2757 | WP_011004048.1 | type VI secretion system tip protein VgrG |
| RS_RS20710 | 944694..945827 | + | 1134 | WP_011004049.1 | DUF4123 domain-containing protein | ||
| RS_RS20715 | 945805..948582 | + | 2778 | WP_011004050.1 | hypothetical protein | ||
| RS_RS20720 | 948796..949701 | + | 906 | WP_119918803.1 | MFS transporter | ||
| RS_RS20725 | 949909..950547 | + | 639 | WP_011004052.1 | DUF4123 domain-containing protein | ||
| RS_RS20730 | 950578..953901 | + | 3324 | WP_043876838.1 | hypothetical protein | ||
| RS_RS20735 | 954467..954850 | + | 384 | WP_011004054.1 | hypothetical protein | ||
| RS_RS20740 | 954855..955457 | + | 603 | pseudogene | hypothetical protein | ||
| RS_RS20745 | 955610..956953 | + | 1344 | WP_011004056.1 | glycoside hydrolase family 10 protein | ||
| T6CP000211 | TssA | RS_RS20750 (tssA) | 957087..958166 | + | 1080 | WP_038962316.1 | type VI secretion system protein TssA |
| RS_RS20755 | 958239..959207 | - | 969 | WP_043876947.1 | hypothetical protein | ||
| T6CP011416 | TagL | RS_RS20760 | 959306..960139 | - | 834 | WP_011004059.1 | OmpA family protein |
| RS_RS20765 (tagF) | 960142..961128 | - | 987 | WP_011004060.1 | type VI secretion system-associated protein TagF | ||
| T6CP000212 | TssM | RS_RS20770 (tssM) | 961125..965066 | - | 3942 | WP_011004061.1 | type VI secretion system membrane subunit TssM |
| RS_RS20775 | 965104..966591 | - | 1488 | WP_011004062.1 | hypothetical protein | ||
| T6CP011415 | PAAR | RS_RS20780 | 966602..966871 | - | 270 | WP_011004063.1 | PAAR domain-containing protein |
| RS_RS20785 | 966911..967759 | - | 849 | WP_011004064.1 | M15 family metallopeptidase | ||
| RS_RS20790 | 968046..968792 | + | 747 | WP_011004065.1 | hypothetical protein | ||
| T6CP000213 | TssI | RS_RS20795 | 968930..971950 | + | 3021 | WP_011004066.1 | type VI secretion system tip protein VgrG |
| RS_RS20800 | 971966..973637 | + | 1672 | pseudogene | hypothetical protein | ||
| RS_RS26230 | 973730..974650 | + | 921 | WP_162014412.1 | hypothetical protein | ||
| RS_RS27155 | 974849..975778 | + | 930 | WP_011004069.1 | hypothetical protein | ||
| RS_RS26235 | 975927..976862 | + | 936 | WP_162014413.1 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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