Detailed information of component protein


Summary

Component ID T6CP000211
Component protein type TssA
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (360 a.a.); Nucleotide sequence (1080 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20750
Coordinate (Strand) 957087..958166 (+)
NCBI ID WP_038962316.1
RefSeq NC_003296
Uniprot ID A0A223GUP5_9RALS, A0A0S4WK95_RALSL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 8.1e-38 10..132
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-359 MATLQFDQLLAPLSGPTPCGEDMLFSTEFDTIQEARRFDDPSLDQGDWVTEIKEADWRAVVSESTALLQKRTKDLRLAAWLTEALSKERGFAGLREGYELIAGLCEQYWEHLFPLPEPDDPEARTGSMAWLAGRSSQLIREVPLVEAGNGGFNYVDWEVATNLIEAIRRDPDQADELSRGKVTQDQFEAARRASSSAFYTALYNDVVGCGAALLRLESVLDGRAGDHAPSFRAAREALQAVRALVERFGGKTETKPSAVEPKATTAPQQTAQPVAEAVPDTVVGPIRTRAQALNQLREVAEFFRQTEPHSPVAYLAARAAKWGDMPLHAWLRAVVKDDATLSQIEELLGLGDSPADDAS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9876 - -


  Protein sequence: 360 a.a.    

>T6CP000211 NC_003296:957087-958166 [Ralstonia solanacearum GMI1000] [TssA]
MATLQFDQLLAPLSGPTPCGEDMLFSTEFDTIQEARRFDDPSLDQGDWVTEIKEADWRAVVSESTALLQKRTKDLRLAAW
LTEALSKERGFAGLREGYELIAGLCEQYWEHLFPLPEPDDPEARTGSMAWLAGRSSQLIREVPLVEAGNGGFNYVDWEVA
TNLIEAIRRDPDQADELSRGKVTQDQFEAARRASSSAFYTALYNDVVGCGAALLRLESVLDGRAGDHAPSFRAAREALQA
VRALVERFGGKTETKPSAVEPKATTAPQQTAQPVAEAVPDTVVGPIRTRAQALNQLREVAEFFRQTEPHSPVAYLAARAA
KWGDMPLHAWLRAVVKDDATLSQIEELLGLGDSPADDAS*

  Nucleotide sequence: 1080 bp    

>T6CP000211 NC_003296:957087-958166 [Ralstonia solanacearum GMI1000] [TssA]
ATGGCAACCCTCCAGTTCGACCAGCTTCTTGCTCCGCTCTCCGGCCCCACGCCTTGCGGCGAGGACATGCTCTTCTCCAC
CGAATTCGACACCATCCAGGAAGCGCGGCGCTTCGACGACCCCTCGCTCGATCAGGGCGACTGGGTGACCGAGATCAAGG
AGGCGGACTGGCGCGCCGTCGTCTCGGAAAGCACGGCGTTGCTGCAGAAGCGCACCAAGGACCTGCGCCTGGCCGCGTGG
CTGACCGAGGCGCTGTCCAAGGAGCGCGGCTTCGCCGGCCTGCGCGAGGGCTACGAGCTGATCGCGGGGCTGTGCGAGCA
GTATTGGGAGCACCTCTTCCCGCTGCCCGAGCCGGATGATCCCGAGGCGCGCACCGGCAGCATGGCGTGGCTGGCGGGGC
GCTCCAGCCAGCTGATCCGCGAGGTGCCGCTGGTGGAGGCGGGCAACGGCGGCTTCAACTATGTCGACTGGGAGGTCGCC
ACCAACCTGATCGAGGCGATCCGGCGCGATCCGGACCAGGCGGATGAGCTGTCGCGCGGCAAGGTCACGCAGGATCAGTT
CGAGGCGGCGCGCCGCGCGTCGTCTTCGGCGTTCTACACGGCGCTGTACAACGACGTGGTGGGTTGCGGCGCGGCGCTGC
TGCGGCTGGAATCCGTGCTGGACGGGCGCGCCGGGGATCATGCGCCGAGCTTCCGCGCCGCGCGCGAGGCCTTGCAGGCA
GTCCGCGCCCTGGTCGAGCGTTTCGGCGGCAAGACGGAAACCAAGCCCAGCGCGGTCGAGCCCAAGGCCACCACCGCGCC
GCAGCAGACCGCCCAGCCCGTCGCCGAGGCCGTGCCCGATACCGTGGTCGGCCCGATCCGCACGCGCGCCCAGGCGCTGA
ACCAGTTGCGCGAAGTGGCTGAGTTCTTCCGCCAGACCGAGCCTCACAGCCCGGTGGCCTATCTCGCCGCACGCGCGGCC
AAGTGGGGCGATATGCCGCTGCACGCCTGGCTGCGCGCGGTGGTCAAGGACGATGCGACGCTGTCGCAGATCGAAGAACT
GCTGGGGTTGGGCGATTCGCCGGCGGATGACGCGAGCTGA