Detailed information of component protein


Summary

Component ID T6CP000209
Component protein type TssH
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (906 a.a.); Nucleotide sequence (2718 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20700
Coordinate (Strand) 939182..941899 (+)
NCBI ID WP_011004047.1
RefSeq NC_003296
Uniprot ID Q8XRT2_RALSO
KEGG ID rso:RSp0749
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 6.2e-42 635..803
AAA_lid_9 PF17871.3 2.7e-26 374..473
ClpB_D2-small PF10431.11 3.9e-10 811..886
Clp_N PF02861.22 1.5e-09 23..75
AAA PF00004.31 1.9e-09 232..344
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-905 MATPLKTLIAKLNATSRQAAERAASLCMARGNYEVDLEHLFLALLENRRSDFAVAARASGIDPAALQRDLEAEVGRFQTGNTRTPAFSPYLPKLFEHAWLIASLDSQITRIRSGHLLLALLTEPSLAALAERGSRLFGRFEVERLKHDFERMTTGSDEQEQAVDFADGTAVQTDDGRTAPAAALGATPALDQFTVDLTQAAREGRIDPVIGRDAEIRQVVDILMRRRQNNPILTGEAGVGKTAVVEGLALRVAANDVPPPLRGVTLRTLDMGLLQAGASVKGEFENRLKNVIDEVKKSPKPIILFIDEAHTIIGAGGQAGQNDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQVVKVDEPSEPLAAAMLRGMAPLMERHFGVRVLDEAITEAVRLSHRYISGRQLPDKAVSVLDTACAKVALGQSATPGAIEDDQKLLERLLGEIAALQREQSAGAAHDARLEALQAKRAELEQRIAQNTERLARERALVARIQALREARESDAAQAGAQDAEPVAANSDVVALSPKRARAADAPDELATLLAELRALQGEAPMVPLQVDGHVVSEIVSAWTGIPLGRMVKDELRTVLNLKPLLAARVIGQDHALDAIAQRVRTASANLEDPNKPRGVFLFAGPSGVGKTETALALADILYGGERKLITINMSEYQEAHSVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKAHPDVLEMFFQVFDKGEMDDAEGRPIDFRNTIIILTSNVGSSAIMQACLNQPAEALPDADTLAETLRPVLYKAFKPAFLGRTKVVPYYPIADDVLAEIITLKLGRIRDRVAANHKATFQWDNALVESVLARCTEVDAGARAVDHILNGTLLPEIAEAVLTRMAEGGSVEKIKVSVGKTGEFKYRIN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9851 - -


  Protein sequence: 906 a.a.    

>T6CP000209 NC_003296:939182-941899 [Ralstonia solanacearum GMI1000] [TssH]
MATPLKTLIAKLNATSRQAAERAASLCMARGNYEVDLEHLFLALLENRRSDFAVAARASGIDPAALQRDLEAEVGRFQTG
NTRTPAFSPYLPKLFEHAWLIASLDSQITRIRSGHLLLALLTEPSLAALAERGSRLFGRFEVERLKHDFERMTTGSDEQE
QAVDFADGTAVQTDDGRTAPAAALGATPALDQFTVDLTQAAREGRIDPVIGRDAEIRQVVDILMRRRQNNPILTGEAGVG
KTAVVEGLALRVAANDVPPPLRGVTLRTLDMGLLQAGASVKGEFENRLKNVIDEVKKSPKPIILFIDEAHTIIGAGGQAG
QNDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQVVKVDEPSEPLAAAMLRGMAPLMERHFGVRVLDEAIT
EAVRLSHRYISGRQLPDKAVSVLDTACAKVALGQSATPGAIEDDQKLLERLLGEIAALQREQSAGAAHDARLEALQAKRA
ELEQRIAQNTERLARERALVARIQALREARESDAAQAGAQDAEPVAANSDVVALSPKRARAADAPDELATLLAELRALQG
EAPMVPLQVDGHVVSEIVSAWTGIPLGRMVKDELRTVLNLKPLLAARVIGQDHALDAIAQRVRTASANLEDPNKPRGVFL
FAGPSGVGKTETALALADILYGGERKLITINMSEYQEAHSVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKA
HPDVLEMFFQVFDKGEMDDAEGRPIDFRNTIIILTSNVGSSAIMQACLNQPAEALPDADTLAETLRPVLYKAFKPAFLGR
TKVVPYYPIADDVLAEIITLKLGRIRDRVAANHKATFQWDNALVESVLARCTEVDAGARAVDHILNGTLLPEIAEAVLTR
MAEGGSVEKIKVSVGKTGEFKYRIN*

  Nucleotide sequence: 2718 bp    

>T6CP000209 NC_003296:939182-941899 [Ralstonia solanacearum GMI1000] [TssH]
ATGGCCACCCCACTCAAGACCTTGATCGCCAAGCTGAACGCGACCAGCCGGCAGGCCGCCGAGCGCGCGGCGTCGCTGTG
CATGGCGCGCGGCAACTATGAAGTCGACCTGGAGCACCTGTTCCTGGCGCTGCTCGAAAACCGCCGCAGCGATTTCGCTG
TCGCGGCGCGCGCCAGCGGCATCGATCCGGCCGCGCTGCAGCGCGACCTGGAGGCCGAGGTGGGGCGCTTCCAGACCGGC
AACACGCGCACGCCGGCGTTCTCGCCGTATCTGCCCAAGCTGTTCGAGCATGCGTGGCTGATCGCCTCGCTGGATTCGCA
GATCACGCGCATCCGCTCGGGCCACCTGCTGCTCGCGCTGCTGACCGAGCCCAGCCTGGCCGCGCTGGCCGAACGCGGCT
CGCGGCTGTTCGGCCGCTTCGAGGTGGAGCGGCTCAAGCACGATTTCGAGCGCATGACCACCGGCTCCGACGAGCAGGAG
CAGGCCGTCGATTTCGCCGACGGCACGGCCGTGCAGACGGACGATGGCCGCACCGCGCCCGCCGCCGCGCTGGGCGCGAC
ACCGGCCCTCGACCAGTTCACCGTGGACCTGACGCAGGCCGCCCGCGAAGGCCGCATCGATCCGGTGATCGGGCGCGACG
CCGAGATCCGCCAGGTGGTCGACATCCTGATGCGCCGGCGCCAGAACAACCCCATCCTGACCGGCGAGGCCGGTGTCGGC
AAGACGGCGGTGGTGGAGGGGCTTGCGCTGCGCGTGGCCGCCAACGACGTGCCGCCGCCGCTGCGGGGCGTGACGCTGCG
CACGCTCGACATGGGCCTGCTGCAGGCCGGCGCGAGTGTGAAGGGCGAGTTCGAGAACCGCCTGAAGAACGTGATCGACG
AGGTCAAGAAGAGCCCGAAGCCGATCATTCTGTTCATCGACGAGGCGCACACCATCATCGGCGCGGGCGGGCAGGCCGGG
CAGAACGATGCGGCCAATCTGCTCAAGCCGGCACTGGCGCGCGGTGAGCTGCGCACCATCGCCGCGACCACCTGGAGCGA
GTACAAGAAGTATTTCGAAAAGGACGCCGCCCTCGCGCGCCGCTTCCAGGTCGTCAAGGTCGACGAGCCGAGCGAGCCGC
TGGCCGCGGCCATGCTGCGCGGCATGGCGCCGCTGATGGAGCGCCACTTCGGCGTGCGCGTGCTGGACGAGGCTATCACC
GAGGCCGTGCGCCTGTCGCATCGCTACATCAGCGGGCGGCAGCTGCCGGACAAGGCGGTCAGCGTGCTGGACACGGCGTG
CGCCAAGGTGGCACTGGGCCAGAGCGCCACGCCGGGCGCCATCGAAGACGACCAGAAATTGCTGGAGCGTCTGCTGGGCG
AGATCGCCGCGCTGCAGCGCGAGCAGAGCGCCGGCGCGGCGCACGATGCCCGCCTCGAAGCGCTGCAGGCCAAGCGCGCC
GAGCTGGAGCAGCGCATCGCGCAGAACACCGAGCGCCTGGCCCGGGAGCGTGCGCTGGTCGCGCGCATCCAGGCGCTGCG
CGAGGCGCGCGAAAGCGACGCGGCGCAGGCCGGCGCGCAGGACGCGGAGCCGGTGGCGGCCAACAGCGATGTGGTGGCGC
TGTCGCCCAAGCGCGCCCGTGCCGCCGACGCGCCGGACGAACTGGCCACGCTGCTGGCCGAGCTGCGCGCGCTGCAGGGC
GAGGCGCCCATGGTGCCGCTGCAGGTGGACGGCCATGTCGTGTCGGAGATCGTCTCGGCCTGGACGGGCATTCCGCTGGG
CCGCATGGTCAAGGACGAACTGCGCACCGTGCTGAACCTGAAGCCGCTGCTGGCCGCGCGCGTGATCGGCCAGGACCACG
CGCTGGACGCCATCGCCCAGCGCGTGCGCACCGCCTCGGCCAACCTGGAAGACCCGAACAAGCCGCGCGGCGTGTTCCTG
TTCGCGGGCCCGTCGGGCGTGGGCAAGACCGAGACGGCGCTGGCGCTGGCCGACATCCTCTACGGCGGCGAGCGCAAGCT
CATCACCATCAACATGAGCGAGTACCAGGAGGCGCACAGCGTCTCGGGCCTGAAGGGCTCGCCGCCGGGTTACGTGGGCT
ACGGCGAGGGCGGTGTGCTGACCGAGGCGGTGCGCCGCAACCCGTACAGCGTGGTGCTGCTGGACGAAGTCGAGAAAGCC
CACCCCGACGTGCTGGAGATGTTCTTCCAGGTGTTCGACAAGGGCGAGATGGACGACGCCGAGGGCCGCCCCATCGACTT
CCGCAACACCATCATCATCCTGACCTCGAACGTGGGCTCGTCGGCCATCATGCAGGCCTGCCTGAACCAGCCCGCCGAGG
CGCTGCCCGACGCCGATACCCTGGCCGAGACGCTGCGGCCGGTGCTGTACAAGGCCTTCAAGCCGGCCTTCCTGGGGCGG
ACCAAGGTGGTGCCGTACTACCCGATCGCCGACGACGTGCTGGCCGAGATCATCACGCTCAAGCTCGGCCGCATCCGCGA
CCGCGTGGCCGCCAACCACAAGGCGACGTTCCAGTGGGACAACGCGCTGGTGGAATCCGTGCTGGCCCGCTGCACCGAAG
TGGATGCGGGCGCGCGCGCGGTCGACCACATCCTCAACGGCACGCTGCTGCCCGAGATCGCCGAAGCGGTGCTCACGCGC
ATGGCCGAAGGCGGCAGCGTCGAGAAGATCAAGGTCAGTGTCGGCAAGACCGGCGAGTTCAAATACCGCATCAACTGA