Detailed information of component protein


Summary

Component ID T6CP000213
Component protein type TssI
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (1007 a.a.); Nucleotide sequence (3021 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20795
Coordinate (Strand) 968930..971950 (+)
NCBI ID WP_011004066.1
RefSeq NC_003296
Uniprot ID Q8XRR3_RALSO
KEGG ID rso:RSp0768
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 2e-69 37..354
DUF2345 PF10106.11 8.8e-39 772..928
T6SS_Vgr PF13296.8 4.4e-33 616..724
Phage_base_V PF04717.14 9.7e-16 471..541
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1006 MDVTTLLQNLFAPAHRLYALEGEGPIAELAVEAWLGREALSELFEWRVVAASANARFALESFIGQRVTLVTTLADGTQARRTGLIRQAEQLGADGSLARYRLTVVPWFWLATQQRHSQVFQNRPLADILEQVLSPYAPYAAWRFAAGAEDRMAAFGTRSHIAQFRETDYRFVTRLLAEAGLGFTTVEDEQAPSGHALLIFADSPRLAEDTASAAEGGIRYHRAHSQEERDAIQALICHSRTAVGGVAVAAWDAQAKRAFRAHVPSRFCGIAGSPDAYLSISPSLAPDTANAQRIAEQVMESVEARARLFIGRGSVRTLRSGTRLKIVDCPHLPKDVDGPYPLLIDLVEHCGINNLTADTQATLARKLGGLDAALTFDTPPSPPESGPGPFGFMAPHEVIERFTPTADLLTAARAHGYAGQFRAGDARRPWRPVVSHPDTGRLYAEPTAKGVHTAIVVGPNGETEASGDAEHHTTPRGQVRVRFPWQQGERPDDRSTGWLLVAQRQAGAGMGWQWLPRIGQEVLVKFQEDDIDQPVVIGALYNGQGEAGIAPTPGGQVAKGVRQEADPETLYRLGSDSAASAQGNLAAGHSPAWHGLGTEAEGHRNAAALSGFKSAEHGGRGANLLVFDDSDGQLRTQLATTQAYSQLNLGHLIHQQDNRRGSFRGQGFELRTDGYGAVRGHAGVLLTTYRDATSGKAVPTGDNAAGIALIRQAKQLTSSLSQGATTHQTAALSTAKDDSAPLAQQEKAASGMVDGKALDAARQDAAAGNTTTQGKVPHQSDATVQLAGRAGLVAIAGQDLQFASGESIALASGQDTNVAVGKQARLHAGQGIGVAAGLSKAGDDNIGLQLTAGQDNLDVQAQHDALGLLSKDDLKLVSANLHVDFAAAKRIRLATAAGASITLEGGNIIVETPGRITYKAAQRSFAGPTHASRELNTWSHSAFDDRYVLRDQVTHEPLRNTQVELRRGDGAVLKLVTDGEGRLPVQKGISMERVQLRVLGTLSGKT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9864 - -


  Protein sequence: 1007 a.a.    

>T6CP000213 NC_003296:968930-971950 [Ralstonia solanacearum GMI1000] [TssI]
MDVTTLLQNLFAPAHRLYALEGEGPIAELAVEAWLGREALSELFEWRVVAASANARFALESFIGQRVTLVTTLADGTQAR
RTGLIRQAEQLGADGSLARYRLTVVPWFWLATQQRHSQVFQNRPLADILEQVLSPYAPYAAWRFAAGAEDRMAAFGTRSH
IAQFRETDYRFVTRLLAEAGLGFTTVEDEQAPSGHALLIFADSPRLAEDTASAAEGGIRYHRAHSQEERDAIQALICHSR
TAVGGVAVAAWDAQAKRAFRAHVPSRFCGIAGSPDAYLSISPSLAPDTANAQRIAEQVMESVEARARLFIGRGSVRTLRS
GTRLKIVDCPHLPKDVDGPYPLLIDLVEHCGINNLTADTQATLARKLGGLDAALTFDTPPSPPESGPGPFGFMAPHEVIE
RFTPTADLLTAARAHGYAGQFRAGDARRPWRPVVSHPDTGRLYAEPTAKGVHTAIVVGPNGETEASGDAEHHTTPRGQVR
VRFPWQQGERPDDRSTGWLLVAQRQAGAGMGWQWLPRIGQEVLVKFQEDDIDQPVVIGALYNGQGEAGIAPTPGGQVAKG
VRQEADPETLYRLGSDSAASAQGNLAAGHSPAWHGLGTEAEGHRNAAALSGFKSAEHGGRGANLLVFDDSDGQLRTQLAT
TQAYSQLNLGHLIHQQDNRRGSFRGQGFELRTDGYGAVRGHAGVLLTTYRDATSGKAVPTGDNAAGIALIRQAKQLTSSL
SQGATTHQTAALSTAKDDSAPLAQQEKAASGMVDGKALDAARQDAAAGNTTTQGKVPHQSDATVQLAGRAGLVAIAGQDL
QFASGESIALASGQDTNVAVGKQARLHAGQGIGVAAGLSKAGDDNIGLQLTAGQDNLDVQAQHDALGLLSKDDLKLVSAN
LHVDFAAAKRIRLATAAGASITLEGGNIIVETPGRITYKAAQRSFAGPTHASRELNTWSHSAFDDRYVLRDQVTHEPLRN
TQVELRRGDGAVLKLVTDGEGRLPVQKGISMERVQLRVLGTLSGKT*

  Nucleotide sequence: 3021 bp    

>T6CP000213 NC_003296:968930-971950 [Ralstonia solanacearum GMI1000] [TssI]
ATGGACGTAACGACCCTTCTCCAGAACCTCTTCGCTCCCGCGCACCGCCTGTACGCGCTGGAAGGCGAAGGCCCGATCGC
GGAGCTGGCGGTGGAGGCGTGGCTCGGTCGCGAGGCGTTGTCCGAGCTGTTCGAATGGCGGGTGGTAGCGGCCAGCGCCA
ATGCGCGCTTCGCCCTCGAATCGTTCATCGGCCAGCGCGTGACGCTGGTCACCACGCTGGCCGACGGCACGCAGGCCCGG
CGCACCGGCCTGATCCGGCAGGCCGAGCAGCTTGGCGCCGATGGCAGCCTCGCGCGCTATCGCCTGACCGTGGTGCCGTG
GTTCTGGCTCGCCACCCAACAGCGGCACAGCCAGGTGTTCCAGAACCGGCCGCTGGCGGACATCCTCGAACAGGTGCTGT
CGCCGTATGCGCCCTATGCCGCATGGCGCTTCGCCGCCGGCGCGGAAGACCGCATGGCGGCGTTCGGCACGCGTAGCCAC
ATCGCTCAGTTTCGCGAGACCGACTACCGCTTCGTCACGCGCCTGCTGGCCGAGGCGGGTCTGGGCTTCACCACCGTCGA
GGACGAGCAGGCCCCCAGCGGCCACGCGCTGCTGATCTTTGCCGACAGCCCCCGGCTGGCCGAGGACACGGCATCCGCTG
CCGAGGGCGGCATCCGCTACCACCGCGCGCACAGCCAGGAAGAACGCGACGCCATCCAGGCGTTGATCTGCCATAGCCGC
ACAGCGGTCGGCGGAGTGGCGGTGGCGGCCTGGGATGCGCAGGCCAAGCGGGCCTTCCGCGCGCACGTTCCCTCCCGGTT
TTGCGGTATCGCGGGCAGCCCGGATGCCTATCTGTCGATCAGCCCGTCGCTGGCGCCGGATACGGCCAACGCGCAGCGCA
TCGCCGAGCAGGTGATGGAGAGCGTCGAAGCGCGCGCTCGCCTGTTCATCGGACGCGGCTCGGTGCGCACGCTGCGCAGC
GGCACGCGACTGAAGATCGTGGACTGCCCGCACCTGCCGAAGGACGTGGACGGCCCGTATCCGTTGCTGATCGACCTGGT
GGAGCACTGCGGCATCAACAACCTGACCGCCGATACGCAGGCAACATTGGCCCGGAAGCTGGGCGGGCTCGACGCCGCAC
TGACCTTCGACACGCCGCCCAGCCCCCCCGAATCCGGCCCCGGCCCGTTCGGCTTCATGGCGCCACACGAGGTCATCGAA
CGCTTCACGCCGACGGCGGACCTGCTCACCGCTGCGCGCGCCCACGGCTACGCCGGCCAATTCCGCGCGGGTGACGCACG
CCGGCCGTGGCGACCGGTCGTGTCGCATCCCGACACCGGCCGCCTGTACGCCGAGCCCACCGCCAAGGGCGTACACACGG
CCATCGTGGTCGGCCCGAACGGCGAGACCGAGGCCAGTGGCGATGCGGAGCACCACACCACTCCACGGGGCCAGGTCCGC
GTCCGCTTCCCGTGGCAGCAGGGCGAACGTCCGGATGACCGCAGCACCGGCTGGCTGCTGGTCGCGCAGCGGCAGGCTGG
GGCCGGCATGGGCTGGCAGTGGCTGCCGCGCATCGGCCAGGAAGTGTTGGTCAAGTTCCAGGAAGACGATATCGACCAGC
CGGTGGTGATCGGCGCGCTCTACAACGGCCAGGGCGAAGCGGGCATCGCGCCGACCCCGGGCGGGCAGGTCGCCAAGGGC
GTCAGGCAGGAGGCCGACCCGGAGACCCTCTACCGTTTGGGCAGCGACAGCGCCGCCAGCGCGCAGGGCAACCTCGCCGC
CGGCCACAGCCCCGCATGGCACGGCCTGGGCACCGAGGCCGAAGGCCACCGCAACGCCGCCGCCCTCAGCGGCTTCAAGA
GCGCCGAGCATGGCGGGCGCGGCGCCAACCTGCTCGTCTTCGACGACAGCGACGGGCAGTTGCGCACGCAGCTCGCCACC
ACGCAGGCTTACAGCCAACTCAACCTGGGCCACCTGATCCACCAGCAGGACAACCGGCGCGGCAGCTTCCGGGGCCAGGG
CTTCGAGTTGCGCACCGATGGCTATGGCGCGGTGCGCGGCCACGCCGGTGTGCTGCTGACCACCTACCGCGATGCCACGA
GCGGCAAGGCTGTGCCGACCGGCGACAACGCCGCCGGCATCGCGCTGATCCGGCAGGCCAAGCAACTGACGTCATCGTTG
AGCCAGGGCGCCACTACGCACCAGACCGCAGCGCTGTCCACCGCGAAGGACGACAGCGCACCGCTGGCCCAGCAGGAAAA
GGCCGCCTCGGGCATGGTCGACGGCAAAGCGCTCGATGCGGCCCGGCAGGACGCGGCCGCAGGCAACACCACCACGCAAG
GCAAGGTGCCGCACCAGAGCGATGCGACTGTGCAGCTGGCCGGCCGCGCGGGCCTGGTGGCGATCGCCGGGCAGGATCTG
CAATTCGCCAGCGGCGAGAGCATCGCACTGGCCAGCGGCCAGGACACCAACGTCGCGGTGGGCAAGCAGGCGCGCCTCCA
CGCGGGGCAGGGCATCGGCGTGGCAGCGGGGCTGTCGAAGGCGGGGGACGACAACATCGGCCTGCAGCTGACGGCGGGGC
AGGACAACCTCGACGTGCAGGCGCAGCATGACGCGCTCGGGCTGCTGTCCAAGGACGACCTGAAGCTGGTGTCGGCGAAT
CTGCATGTGGACTTCGCGGCGGCCAAGCGGATCCGGCTGGCGACGGCGGCGGGGGCGTCGATCACGCTGGAGGGCGGAAA
CATCATCGTCGAGACGCCGGGGCGGATTACGTACAAGGCGGCGCAGCGCAGCTTTGCGGGGCCCACGCACGCGTCGCGCG
AGCTGAACACGTGGTCGCACAGTGCGTTTGACGATCGATATGTTTTGCGCGACCAAGTCACGCACGAGCCGCTGCGGAAT
ACGCAGGTCGAATTGCGGCGCGGTGACGGTGCTGTACTCAAGCTGGTCACCGATGGAGAGGGCCGCCTCCCTGTGCAGAA
GGGTATTTCCATGGAGCGTGTGCAACTGCGCGTGCTGGGAACGCTCTCCGGCAAAACCTAA