Detailed information of component protein


Summary

Component ID T6CP000210
Component protein type TssI
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (919 a.a.); Nucleotide sequence (2757 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20705
Coordinate (Strand) 941920..944676 (+)
NCBI ID WP_011004048.1
RefSeq NC_003296
Uniprot ID Q8XRT1_RALSO
KEGG ID rso:RSp0750
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.9e-74 47..362
DUF2345 PF10106.11 6.5e-41 728..884
T6SS_Vgr PF13296.8 8.4e-25 586..689
Phage_base_V PF04717.14 1.5e-11 479..528
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-918 MVSPTELANLLRAAFTQANRLLRLDTPLGPNALLPERLDVAEHLDDGGFRLELTALSDDADLDPARLLGQPVRLDLLTQQSRGALRPFHGHVTRFEQLGANGGLVRYRLVIEPWLAFLRHRRDSFLFQDQSVIEVIDSLFGDYNGQGRLAPAWRWSLRDASVYTRRSVITQYEESDFDFVTRLLAEEGLFYFFEHEAVEGDALGVHRMVIADANDVFTDNAQAAIRFGRADATANEDVIDRWQGVRRWQTNAVSIASWDYRANTQRSAQLGAQQWRDGIPQLTLQDTDYPGQYWFEDSAQAQRSARLVMEALELRDKQFDGEGSVRTLALGTRFQLVDHYEHGDDGEARFAVLSVLHRARNNFNERFGQVLTQALGALSGADAQDAAGNAGTDATFYRNHFTVVHDTVPYRPQQRDAQGRALHPRPTVEGSQTALVVGTGGPVHTDRDHRIKVQFHWQRGARSASRQPHPAGDDNARAEAGLGCWVRVAAPVAGPNWGGVALPRVGQEVLVEFVQGDIDRPVVVGAAYNGSGQRDAQYNQNQAGAAGATGNAPAWFAGDSKSQGAYAHNAVLSGIKTQALAGSQTGASGYSQLVFDDTEGQGRTQLSTTQAATALTLGHQKEQIDSARQADLGHGAALATNDSGSVRAGAGLLLTAHAADTGAALLDSEGAASQIEALSELAESLADVARKQQAGLPEEPAAQDLPALKQLRHTTEVLRHAESAGKGASAIAYSEPHLQVSAPKGIAATTPADAVLVAGTQIALAAKQDANLAAGGNLAMAVADGLTLFAHGKAVGQSSDAARGIAMHAASGKVSLSALKGESRLVAEKAVTVASTQGNVTAEGKARVLVNAAGAQIRVTNGTIELHAPGKTIFKGAGHVFVGPGGGAVSNTLAQGNLKGCGTQEGNAATKGTGSISR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9916 - -


  Protein sequence: 919 a.a.    

>T6CP000210 NC_003296:941920-944676 [Ralstonia solanacearum GMI1000] [TssI]
MVSPTELANLLRAAFTQANRLLRLDTPLGPNALLPERLDVAEHLDDGGFRLELTALSDDADLDPARLLGQPVRLDLLTQQ
SRGALRPFHGHVTRFEQLGANGGLVRYRLVIEPWLAFLRHRRDSFLFQDQSVIEVIDSLFGDYNGQGRLAPAWRWSLRDA
SVYTRRSVITQYEESDFDFVTRLLAEEGLFYFFEHEAVEGDALGVHRMVIADANDVFTDNAQAAIRFGRADATANEDVID
RWQGVRRWQTNAVSIASWDYRANTQRSAQLGAQQWRDGIPQLTLQDTDYPGQYWFEDSAQAQRSARLVMEALELRDKQFD
GEGSVRTLALGTRFQLVDHYEHGDDGEARFAVLSVLHRARNNFNERFGQVLTQALGALSGADAQDAAGNAGTDATFYRNH
FTVVHDTVPYRPQQRDAQGRALHPRPTVEGSQTALVVGTGGPVHTDRDHRIKVQFHWQRGARSASRQPHPAGDDNARAEA
GLGCWVRVAAPVAGPNWGGVALPRVGQEVLVEFVQGDIDRPVVVGAAYNGSGQRDAQYNQNQAGAAGATGNAPAWFAGDS
KSQGAYAHNAVLSGIKTQALAGSQTGASGYSQLVFDDTEGQGRTQLSTTQAATALTLGHQKEQIDSARQADLGHGAALAT
NDSGSVRAGAGLLLTAHAADTGAALLDSEGAASQIEALSELAESLADVARKQQAGLPEEPAAQDLPALKQLRHTTEVLRH
AESAGKGASAIAYSEPHLQVSAPKGIAATTPADAVLVAGTQIALAAKQDANLAAGGNLAMAVADGLTLFAHGKAVGQSSD
AARGIAMHAASGKVSLSALKGESRLVAEKAVTVASTQGNVTAEGKARVLVNAAGAQIRVTNGTIELHAPGKTIFKGAGHV
FVGPGGGAVSNTLAQGNLKGCGTQEGNAATKGTGSISR*

  Nucleotide sequence: 2757 bp    

>T6CP000210 NC_003296:941920-944676 [Ralstonia solanacearum GMI1000] [TssI]
ATGGTCTCACCGACCGAGCTGGCCAACCTGCTGCGGGCCGCCTTCACGCAGGCCAATCGGCTGCTGCGCCTGGATACGCC
GCTCGGGCCGAACGCGCTGCTGCCCGAGCGGTTGGACGTCGCCGAGCATCTCGACGATGGCGGCTTCCGGCTGGAGCTGA
CCGCGCTGTCGGATGACGCCGATCTCGATCCGGCGCGCCTGCTGGGGCAGCCCGTCCGGCTCGACCTGCTGACCCAGCAG
AGCCGCGGCGCGCTGCGGCCGTTCCATGGCCACGTCACGCGGTTCGAGCAGTTGGGCGCCAACGGCGGCCTGGTGCGCTA
CCGGCTGGTGATCGAACCCTGGCTGGCCTTCCTGCGCCACCGGCGCGACAGCTTCCTGTTCCAGGACCAGTCGGTGATCG
AAGTGATCGACAGCCTGTTCGGCGACTACAACGGCCAGGGCCGCCTGGCGCCGGCGTGGCGCTGGTCGCTGCGCGATGCC
TCGGTGTACACGCGCCGCAGCGTCATCACGCAATACGAAGAAAGCGATTTCGACTTCGTCACCCGCCTGCTGGCCGAGGA
AGGGCTGTTCTACTTCTTCGAGCACGAGGCGGTCGAGGGCGATGCGCTGGGCGTGCACCGCATGGTGATCGCCGATGCCA
ACGATGTCTTCACCGACAACGCGCAGGCCGCGATCCGCTTCGGCCGCGCCGATGCCACCGCCAATGAAGACGTGATCGAC
CGCTGGCAGGGCGTGCGGCGCTGGCAGACCAACGCCGTGTCGATCGCCAGTTGGGACTACCGCGCCAACACCCAGCGCAG
CGCCCAGCTCGGCGCGCAGCAGTGGCGCGACGGCATCCCGCAACTGACGCTGCAGGACACCGACTACCCCGGCCAGTACT
GGTTCGAAGACAGCGCCCAGGCCCAGCGCAGCGCGCGCCTGGTGATGGAAGCGCTGGAGCTGCGCGACAAGCAGTTCGAC
GGCGAAGGCAGCGTGCGCACGCTGGCGCTGGGCACGCGCTTCCAACTGGTTGATCATTATGAGCACGGCGACGACGGCGA
GGCGCGCTTCGCCGTGCTGTCCGTCCTGCACCGGGCACGCAACAACTTCAACGAGCGCTTTGGCCAGGTGCTGACGCAGG
CGCTGGGCGCGCTGTCCGGCGCCGACGCGCAAGACGCCGCGGGCAACGCGGGCACCGACGCCACGTTTTACCGTAACCAC
TTCACGGTCGTGCACGATACCGTGCCGTACCGCCCGCAGCAGCGCGATGCGCAGGGCCGTGCGCTGCATCCGCGTCCGAC
GGTCGAGGGCAGCCAGACCGCACTGGTGGTGGGCACCGGTGGCCCGGTGCATACGGACCGCGATCATCGCATCAAGGTCC
AGTTCCATTGGCAGCGCGGCGCACGCTCGGCCAGTCGGCAGCCGCATCCGGCCGGCGATGACAACGCCCGCGCGGAGGCC
GGCCTGGGCTGCTGGGTGCGCGTGGCGGCGCCGGTGGCCGGGCCCAACTGGGGCGGCGTGGCGTTGCCGCGCGTGGGCCA
GGAAGTGCTGGTGGAATTCGTGCAGGGCGATATCGACCGGCCGGTGGTGGTGGGCGCGGCCTATAACGGCAGCGGCCAGC
GCGACGCGCAGTACAACCAGAACCAGGCCGGCGCGGCGGGCGCGACCGGCAACGCGCCGGCCTGGTTTGCCGGCGACAGC
AAGAGCCAGGGGGCGTACGCGCACAACGCGGTGCTCTCCGGCATCAAGACGCAGGCGCTGGCCGGCAGCCAGACGGGCGC
CTCGGGTTACAGCCAGCTCGTGTTCGACGATACCGAGGGCCAGGGCCGCACGCAGCTGAGCACCACCCAGGCCGCCACCG
CGCTCACGCTGGGGCACCAGAAGGAACAGATCGACAGCGCCCGCCAGGCCGACCTAGGACACGGCGCGGCGCTGGCCACC
AACGACAGCGGCAGCGTGCGCGCCGGCGCGGGCCTGCTGCTCACCGCGCACGCCGCCGACACCGGCGCCGCGCTTCTCGA
CAGCGAAGGCGCCGCCAGCCAGATCGAAGCCCTCAGCGAACTGGCCGAATCGCTGGCCGACGTGGCCCGGAAGCAGCAGG
CCGGCCTGCCGGAAGAGCCTGCCGCGCAAGACCTGCCCGCGCTCAAGCAACTGCGGCACACCACCGAAGTGCTGCGCCAC
GCGGAAAGCGCAGGCAAAGGCGCCTCGGCCATCGCCTACAGCGAACCGCATCTGCAAGTCAGCGCGCCGAAGGGGATTGC
CGCGACCACGCCGGCCGACGCCGTGCTGGTGGCCGGCACCCAGATCGCATTGGCGGCCAAGCAGGACGCCAACCTCGCGG
CGGGCGGCAACCTGGCAATGGCCGTGGCCGACGGCCTGACCCTGTTCGCGCACGGCAAGGCGGTGGGCCAGTCATCCGAC
GCGGCCCGGGGCATCGCGATGCATGCGGCGAGCGGCAAGGTGAGCTTGAGCGCCCTGAAGGGCGAGAGCCGGCTGGTGGC
CGAGAAAGCCGTCACGGTGGCATCCACGCAGGGCAATGTCACGGCGGAGGGCAAGGCGCGCGTGCTGGTGAATGCGGCGG
GCGCGCAGATCCGGGTGACCAACGGGACGATTGAGCTGCATGCGCCGGGGAAGACGATCTTCAAGGGGGCGGGGCATGTG
TTTGTGGGGCCGGGTGGGGGGGCGGTGAGCAATACGCTGGCCCAGGGCAATCTGAAGGGTTGCGGCACCCAGGAAGGTAA
TGCCGCAACGAAAGGCACAGGGTCGATTTCGCGCTGA