Detailed information of component protein


Summary

Component ID T6CP000212
Component protein type TssM
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (1314 a.a.); Nucleotide sequence (3942 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20770
Coordinate (Strand) 961125..965066 (-)
NCBI ID WP_011004061.1
RefSeq NC_003296
Uniprot ID Q8XRR8_RALSO
KEGG ID rso:RSp0763
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.4e-96 488..864
IcmF-related_N PF14331.8 2.1e-83 184..439
IcmF_C PF06744.14 4.3e-22 1137..1239
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MRRFLNFLTHSR
Transmembrane helix 13-31 TLSVLGVLALTIILLLIAL
Outside 32-34 TFE
Transmembrane helix 35-57 IGLVWAGIALGVLLALWLATALW
Inside 58-433 KRWRARRASARLEGMLEQAADKSAAPDKREEVEALRVRLAEAVKTIKRSKLGQLSGDAALYELPWYIVIGNPAAGKSTAVLNSGLQFPFADKGGAVIQGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRREWLGFLDLLKRYRPKAPINGIVVTVSVSELTQNRPEFAINLAKNLRQRVQELTERLEVFAPVYVMFTKADLITGFTEFFGDSEKQERDRVWGASLPFEPDAKPDVAALFDQRFDELCDGLKEISVAQISLHQKNKLSPGLLSFPLEFASIKPALRAFLVTLFDENPFQYKPVFRGFYFTSALQEGATNSASAERIARRFGLSTDGAARSREVFSKNGFFLRDLFSKVIFSDRQTVRQFASPAKTR
Transmembrane helix 434-456 LRIATFFGLVLALGLLLGGWTWS
Outside 457-1313 YMGNRTLTANVQADLDKIVKLQQNRVDLQARLEALDILQDRIEQLERFRGEHPLALSLGLYQGDALQHKLLDEYYNGLRQVMLKPVGGALETFLAEVNTHPEQLAPMVRPPETGAVIATSVTSATATIAASGAAAGAPASASPTATATAAAPAGKRFTDASPSNVEDGYNALKTYLMLSDKRHVEVAHLTDQITRFWRGWLEDNRGNMPREQMIRSAERNLSFFLARVNDDNWPLLEGNLSLIDQTRENLRRVVRGMPARERAYAEIKARASTRYAPMTVARIVGETGGNVIAGSYAVPGTFTKEAWQGYVQPAIREAASKELQTKDWVLNVAARDDLTLEGSPEQIQKTLVTMYKTEYAREWQRFMQGVAVQDFTSFEQAVAGMNLLGDPTNSPIRKVLDTAYDQTSWDNPSMLNAGLKQAKSGVLGWFKQLFARQTPSQINVNVDVGGNTETGSIPMGPVGKEFAGLARVAVMHDDKSLLRGYMDALSKVRTRFNLIKNQGDPGPGARQLMQQTLDGNGSELADTLKYVDEQMLTGMTDSERQTLRPLLVRPLLQSYAVVIRPASVEINKVWNAQVYQPFSQTLAEKYPFAANAKIEASAAEIGQIFGPEGAIAKFANTTIGPLSVRRGDMLAARTWGDLGLAFVPEFTAGFTRWIAPLTGGAAGGAGGAAAAAPQTVFQILPVPSQGTTEYTIEIDGQQLRYRNTPPQWANFVWPNPQGAPGARVTATTFDGRTVELLNEPGRFGLERLIATANRKRRPDGAFDLTWARGDISVSVSLRIISSPQTAGTSGSDSPQGQGLRGLRLPTSIADATASQNTATPPAPAGSRPASAAAATTQATARTLVTQEQGGTAQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9923 - -


  Protein sequence: 1314 a.a.    

>T6CP000212 NC_003296:c965066-961125 [Ralstonia solanacearum GMI1000] [TssM]
MRRFLNFLTHSRTLSVLGVLALTIILLLIALTFEIGLVWAGIALGVLLALWLATALWKRWRARRASARLEGMLEQAADKS
AAPDKREEVEALRVRLAEAVKTIKRSKLGQLSGDAALYELPWYIVIGNPAAGKSTAVLNSGLQFPFADKGGAVIQGIGGT
RNCDWFFTTEGILLDTAGRYSVHEEDRREWLGFLDLLKRYRPKAPINGIVVTVSVSELTQNRPEFAINLAKNLRQRVQEL
TERLEVFAPVYVMFTKADLITGFTEFFGDSEKQERDRVWGASLPFEPDAKPDVAALFDQRFDELCDGLKEISVAQISLHQ
KNKLSPGLLSFPLEFASIKPALRAFLVTLFDENPFQYKPVFRGFYFTSALQEGATNSASAERIARRFGLSTDGAARSREV
FSKNGFFLRDLFSKVIFSDRQTVRQFASPAKTRLRIATFFGLVLALGLLLGGWTWSYMGNRTLTANVQADLDKIVKLQQN
RVDLQARLEALDILQDRIEQLERFRGEHPLALSLGLYQGDALQHKLLDEYYNGLRQVMLKPVGGALETFLAEVNTHPEQL
APMVRPPETGAVIATSVTSATATIAASGAAAGAPASASPTATATAAAPAGKRFTDASPSNVEDGYNALKTYLMLSDKRHV
EVAHLTDQITRFWRGWLEDNRGNMPREQMIRSAERNLSFFLARVNDDNWPLLEGNLSLIDQTRENLRRVVRGMPARERAY
AEIKARASTRYAPMTVARIVGETGGNVIAGSYAVPGTFTKEAWQGYVQPAIREAASKELQTKDWVLNVAARDDLTLEGSP
EQIQKTLVTMYKTEYAREWQRFMQGVAVQDFTSFEQAVAGMNLLGDPTNSPIRKVLDTAYDQTSWDNPSMLNAGLKQAKS
GVLGWFKQLFARQTPSQINVNVDVGGNTETGSIPMGPVGKEFAGLARVAVMHDDKSLLRGYMDALSKVRTRFNLIKNQGD
PGPGARQLMQQTLDGNGSELADTLKYVDEQMLTGMTDSERQTLRPLLVRPLLQSYAVVIRPASVEINKVWNAQVYQPFSQ
TLAEKYPFAANAKIEASAAEIGQIFGPEGAIAKFANTTIGPLSVRRGDMLAARTWGDLGLAFVPEFTAGFTRWIAPLTGG
AAGGAGGAAAAAPQTVFQILPVPSQGTTEYTIEIDGQQLRYRNTPPQWANFVWPNPQGAPGARVTATTFDGRTVELLNEP
GRFGLERLIATANRKRRPDGAFDLTWARGDISVSVSLRIISSPQTAGTSGSDSPQGQGLRGLRLPTSIADATASQNTATP
PAPAGSRPASAAAATTQATARTLVTQEQGGTAQ*

  Nucleotide sequence: 3942 bp    

>T6CP000212 NC_003296:c965066-961125 [Ralstonia solanacearum GMI1000] [TssM]
ATGCGACGCTTCCTCAACTTCCTGACCCACTCACGCACGCTCTCGGTTCTGGGCGTGCTCGCGCTGACGATCATCCTGCT
GCTGATCGCGCTGACCTTCGAGATTGGCCTGGTCTGGGCCGGCATCGCGCTCGGCGTGCTGCTGGCGCTGTGGCTGGCGA
CGGCGCTCTGGAAACGCTGGCGCGCGCGCCGGGCGAGCGCCAGGCTCGAAGGCATGCTGGAGCAGGCCGCCGACAAGAGC
GCCGCGCCGGACAAGCGCGAGGAAGTCGAGGCGCTGCGCGTGCGCCTGGCCGAGGCAGTCAAGACCATCAAGCGCTCCAA
GCTCGGGCAGCTGTCGGGCGATGCGGCCCTCTATGAGCTGCCCTGGTACATCGTGATCGGCAACCCGGCGGCCGGCAAGA
GCACGGCGGTGCTGAACTCGGGCCTGCAGTTTCCGTTCGCCGACAAGGGCGGCGCGGTGATCCAGGGCATCGGCGGCACG
CGCAACTGCGACTGGTTCTTCACCACCGAAGGCATCCTGCTCGACACGGCCGGCCGCTACTCGGTGCACGAGGAAGACCG
CCGCGAATGGCTCGGCTTCCTCGACCTGCTCAAGCGCTACCGCCCCAAGGCGCCGATCAACGGCATCGTCGTGACGGTGA
GCGTGTCGGAGCTCACGCAGAACCGTCCCGAGTTCGCCATCAACCTCGCCAAGAACCTGCGCCAGCGCGTGCAGGAGTTG
ACCGAGCGGCTGGAAGTGTTCGCCCCGGTCTACGTGATGTTCACCAAGGCCGACCTGATCACCGGCTTCACGGAATTCTT
CGGCGACAGCGAGAAGCAGGAGCGCGACCGCGTGTGGGGCGCCTCCCTGCCCTTCGAGCCGGACGCCAAGCCCGACGTGG
CAGCGCTGTTCGACCAGCGCTTCGATGAGCTGTGCGACGGTCTGAAGGAGATCAGCGTGGCGCAGATCTCGCTGCACCAG
AAGAACAAGCTGTCGCCGGGGCTGCTGAGCTTTCCGCTGGAGTTCGCCTCGATCAAGCCGGCGCTGCGCGCCTTCCTGGT
GACGCTGTTCGACGAGAACCCCTTCCAGTACAAGCCGGTGTTCCGCGGCTTCTACTTCACCAGCGCGCTGCAGGAAGGCG
CCACCAACAGCGCCTCGGCCGAGCGCATCGCGCGCCGCTTCGGGCTCAGCACGGACGGCGCCGCGCGCAGCCGCGAGGTG
TTCTCCAAGAACGGCTTCTTCCTGCGCGACCTGTTCTCCAAGGTCATCTTCTCCGACCGCCAGACCGTGCGGCAATTCGC
CAGCCCGGCCAAGACGCGCCTGCGCATCGCCACCTTCTTCGGGCTGGTGCTGGCGTTGGGCCTGCTGCTGGGCGGCTGGA
CGTGGTCGTACATGGGCAACCGCACGCTGACCGCCAACGTGCAGGCCGACCTCGACAAGATCGTCAAGCTGCAGCAGAAC
CGCGTCGACCTGCAAGCCCGGCTGGAAGCGCTGGACATCCTGCAGGACCGCATCGAGCAGCTCGAGCGCTTCCGCGGCGA
GCATCCGCTGGCGCTGTCGCTGGGGCTATACCAGGGCGACGCGCTGCAGCACAAGCTGCTCGACGAGTACTACAACGGCC
TGCGCCAGGTGATGCTCAAGCCGGTGGGCGGCGCGCTCGAGACCTTCCTGGCCGAGGTCAACACGCACCCCGAGCAGCTC
GCGCCGATGGTGCGTCCGCCCGAGACCGGCGCGGTGATCGCCACCTCCGTCACCTCGGCGACGGCCACCATCGCGGCTTC
GGGCGCCGCCGCCGGTGCGCCGGCATCGGCATCGCCCACGGCCACCGCCACGGCCGCCGCGCCGGCCGGCAAGCGCTTCA
CCGATGCCTCGCCCTCCAACGTGGAAGACGGCTACAACGCGCTCAAGACCTACCTGATGCTGTCGGACAAGCGCCACGTG
GAAGTCGCGCACCTGACCGACCAGATCACGCGCTTCTGGCGCGGCTGGCTGGAAGACAACCGGGGCAACATGCCGCGCGA
GCAGATGATCCGCTCGGCCGAGCGCAACCTGTCGTTCTTCCTGGCCCGCGTGAATGACGACAACTGGCCGCTGCTGGAAG
GCAACCTGTCGCTGATCGACCAGACCCGCGAGAACCTGCGCCGCGTGGTGCGCGGCATGCCGGCGCGCGAACGCGCCTAC
GCCGAGATCAAGGCCCGCGCCTCGACCCGCTATGCGCCGATGACGGTCGCGCGCATCGTCGGCGAGACCGGCGGCAACGT
GATCGCCGGCAGCTACGCCGTGCCCGGCACCTTCACCAAGGAGGCATGGCAGGGCTACGTGCAGCCCGCCATCCGCGAGG
CCGCCAGCAAGGAGCTGCAGACCAAGGACTGGGTGCTCAACGTGGCCGCCCGCGACGACCTGACGCTCGAAGGCAGCCCC
GAGCAGATCCAGAAGACCCTGGTCACGATGTACAAGACCGAGTACGCCCGCGAGTGGCAACGCTTCATGCAGGGCGTGGC
CGTGCAGGACTTCACCAGCTTCGAGCAGGCCGTGGCCGGCATGAACCTGCTGGGCGATCCGACCAACTCGCCGATCCGCA
AGGTGCTCGACACCGCCTACGACCAGACCTCGTGGGACAACCCGTCCATGCTCAACGCCGGCCTCAAGCAGGCCAAGTCG
GGCGTGCTGGGCTGGTTCAAGCAGCTCTTCGCGCGGCAGACGCCGTCGCAGATCAACGTCAACGTGGACGTGGGCGGCAA
CACCGAGACCGGCAGCATCCCGATGGGGCCGGTGGGCAAGGAGTTCGCCGGGCTGGCGCGCGTGGCGGTGATGCACGACG
ACAAATCGCTGCTGCGCGGCTACATGGACGCGCTGTCCAAGGTCCGCACCCGCTTCAACCTCATCAAGAACCAGGGCGAC
CCCGGCCCCGGCGCGCGCCAGCTGATGCAGCAGACGCTGGACGGCAACGGCTCCGAGCTGGCCGACACCCTCAAGTACGT
CGATGAGCAGATGCTGACCGGCATGACCGACAGCGAACGTCAGACGCTGCGCCCGCTGCTGGTGCGCCCGCTGCTGCAGT
CGTATGCGGTGGTGATCCGGCCGGCCAGCGTGGAGATCAACAAGGTGTGGAACGCGCAGGTCTACCAGCCGTTCAGCCAG
ACGCTGGCCGAGAAGTATCCGTTTGCCGCCAACGCCAAGATCGAGGCCAGCGCGGCCGAGATCGGCCAGATCTTCGGGCC
GGAGGGCGCCATCGCCAAGTTCGCCAACACGACCATCGGCCCGCTGTCGGTGCGCCGCGGCGACATGCTGGCGGCCCGCA
CCTGGGGTGACCTGGGCCTGGCCTTCGTGCCCGAGTTCACCGCCGGCTTCACGCGCTGGATCGCCCCGCTGACGGGCGGT
GCGGCGGGCGGCGCCGGTGGCGCGGCGGCCGCGGCGCCGCAGACGGTGTTCCAGATTCTGCCGGTGCCGTCGCAGGGCAC
CACCGAATACACCATCGAGATCGACGGCCAGCAACTGCGCTACCGCAACACGCCGCCGCAATGGGCCAACTTCGTCTGGC
CGAATCCGCAGGGCGCGCCGGGCGCGCGCGTGACGGCCACGACCTTCGACGGCCGCACCGTGGAGCTGCTCAACGAACCG
GGCCGCTTCGGCCTGGAGCGCCTGATCGCCACCGCGAACCGCAAGCGTCGCCCCGATGGCGCGTTCGACCTGACGTGGGC
GCGCGGCGACATCAGCGTGTCGGTCAGCCTGCGCATCATCAGCAGCCCGCAGACGGCCGGCACCTCGGGCTCCGACTCGC
CGCAAGGCCAGGGCCTGCGCGGCCTGCGCCTGCCGACCTCGATCGCGGACGCGACCGCCTCGCAGAACACCGCCACGCCG
CCCGCGCCGGCAGGCAGCCGCCCGGCCAGCGCGGCCGCCGCGACGACCCAGGCCACCGCCCGGACCCTCGTCACACAAGA
GCAAGGGGGGACGGCGCAATGA