Detailed information of component protein


Summary

Component ID T6CP000204
Component protein type TssC
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (497 a.a.); Nucleotide sequence (1491 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20675
Coordinate (Strand) 933565..935055 (+)
NCBI ID WP_011004042.1
RefSeq NC_003296
Uniprot ID M4UN33_RALSL, Q8XRT7_RALSO
KEGG ID rse:F504_4477, rso:RSp0744
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 1.8e-139 68..370
VipB_2 PF18945.2 2.4e-46 380..490
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-496 MLDNQSAALAQSAPAAESVNLLDEIVEQSRVAKSDAEHARAKDIIGELVNQVLQGTVVVSDNLSATLDARVAELDRLISSQLSAVMHTPEFQKLEGTWRGLSYLVKETQTSTMLKIKVLNATKRDLVRDFKTAIDFDQSALFKKVYEEEFGTFGGAPFGTLIGDFEVTRQPEDVYFIEQMSHVAAAAHAPFIASSSPELFGLESFADLGKPRDLAKVFDTVEYAKWKSFRESEDSRYVGLTMPRFLGRLPYNPKDGTTVEGFNFVEDVDGTDHSKYLWCNAAWAFGARLTAAFEDFGWCAAIRGVEGGGLVEDLPTHTFKTDDGEIALKCPTEIAITDRREKELSDLGFIPLVHCKNSDYAAFFAAQSVQRPKKYDTDSANANAVLSAQLQYIFSVSRVAHYLKAMMRDKIGSFASAKNVETFLNRWISQYVLLDDNATQEQKAQFPLREASIQVSEVPGKPGTYRSVAFLRPHFQLDELSISLRLVADLPKPTNS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9688 - -


  Protein sequence: 497 a.a.    

>T6CP000204 NC_003296:933565-935055 [Ralstonia solanacearum GMI1000] [TssC]
MLDNQSAALAQSAPAAESVNLLDEIVEQSRVAKSDAEHARAKDIIGELVNQVLQGTVVVSDNLSATLDARVAELDRLISS
QLSAVMHTPEFQKLEGTWRGLSYLVKETQTSTMLKIKVLNATKRDLVRDFKTAIDFDQSALFKKVYEEEFGTFGGAPFGT
LIGDFEVTRQPEDVYFIEQMSHVAAAAHAPFIASSSPELFGLESFADLGKPRDLAKVFDTVEYAKWKSFRESEDSRYVGL
TMPRFLGRLPYNPKDGTTVEGFNFVEDVDGTDHSKYLWCNAAWAFGARLTAAFEDFGWCAAIRGVEGGGLVEDLPTHTFK
TDDGEIALKCPTEIAITDRREKELSDLGFIPLVHCKNSDYAAFFAAQSVQRPKKYDTDSANANAVLSAQLQYIFSVSRVA
HYLKAMMRDKIGSFASAKNVETFLNRWISQYVLLDDNATQEQKAQFPLREASIQVSEVPGKPGTYRSVAFLRPHFQLDEL
SISLRLVADLPKPTNS*

  Nucleotide sequence: 1491 bp    

>T6CP000204 NC_003296:933565-935055 [Ralstonia solanacearum GMI1000] [TssC]
ATGCTTGACAACCAATCCGCCGCGCTCGCGCAGAGCGCACCGGCAGCCGAGTCCGTCAACCTGCTCGACGAGATCGTCGA
GCAGAGCCGCGTCGCCAAGTCGGACGCCGAGCACGCGCGCGCCAAGGACATCATCGGCGAGCTGGTCAACCAGGTGCTGC
AGGGCACGGTGGTGGTGTCCGACAACCTGTCGGCCACGCTGGATGCCCGCGTGGCCGAGCTGGACCGCCTGATCTCGTCG
CAGCTGAGCGCCGTCATGCACACGCCCGAGTTCCAGAAGCTGGAGGGCACGTGGCGCGGCCTGTCGTACCTGGTCAAGGA
AACGCAGACCAGCACGATGCTCAAGATCAAGGTGCTCAACGCCACCAAGCGCGATCTCGTGCGCGACTTCAAGACGGCCA
TCGATTTCGACCAGAGCGCGCTGTTCAAGAAGGTGTACGAAGAAGAATTCGGCACCTTCGGCGGCGCGCCGTTCGGCACG
CTGATCGGCGATTTCGAAGTCACGCGCCAGCCGGAAGACGTGTACTTCATCGAGCAGATGTCGCACGTGGCGGCGGCCGC
GCATGCGCCGTTCATCGCCTCGTCGTCGCCGGAGCTGTTCGGCCTGGAGTCGTTTGCCGACCTGGGCAAGCCGCGTGACC
TGGCCAAGGTGTTCGATACGGTCGAATACGCCAAGTGGAAGTCGTTCCGCGAGTCGGAGGATTCGCGCTACGTGGGCTTG
ACGATGCCGCGCTTCCTGGGCCGGCTGCCGTACAACCCCAAGGACGGCACCACCGTCGAGGGCTTCAACTTCGTCGAAGA
CGTGGACGGCACCGACCACAGCAAATACCTGTGGTGCAACGCGGCCTGGGCGTTCGGCGCGCGGCTGACCGCCGCATTCG
AAGACTTCGGCTGGTGCGCGGCGATCCGCGGCGTGGAAGGCGGCGGGCTGGTCGAAGACCTGCCGACGCATACCTTCAAG
ACCGACGACGGCGAAATCGCGCTCAAGTGCCCGACGGAAATCGCCATCACCGATCGCCGCGAGAAGGAACTGAGCGACCT
GGGCTTCATCCCGCTCGTGCACTGCAAGAACTCGGACTACGCTGCGTTCTTCGCCGCGCAGTCGGTGCAGCGGCCCAAGA
AGTACGACACCGACAGCGCGAATGCCAACGCGGTGCTGTCGGCGCAGCTGCAGTACATCTTCTCGGTATCGCGCGTGGCG
CACTACCTGAAGGCAATGATGCGCGACAAGATCGGCAGCTTTGCGTCGGCCAAGAATGTCGAGACCTTCCTCAACCGGTG
GATTTCGCAGTATGTCCTGCTGGACGACAACGCGACCCAGGAGCAGAAGGCGCAGTTCCCGCTGCGCGAGGCTTCGATCC
AGGTGTCCGAAGTGCCCGGCAAGCCCGGTACGTACCGCTCGGTGGCGTTCCTGCGGCCGCACTTCCAGCTCGATGAACTG
TCGATCTCGCTGCGGCTGGTGGCGGACCTGCCGAAGCCAACCAATTCCTGA