Detailed information of component protein
Summary
| Component ID | T6CP000203 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS00026  (Type i4b) |
| Strain | Ralstonia solanacearum GMI1000 |
| Replicon | plasmid pGMI1000MP |
| Sequence | Protein sequence (171 a.a.); Nucleotide sequence (513 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | RS_RS20670 |
| Coordinate (Strand) | 933060..933572 (+) |
| NCBI ID | WP_011004041.1 |
| RefSeq | NC_003296 |
| Uniprot ID | A0A223GUM7_9RALS, Q8XRT8_RALSO, A0A0K1ZRL9_RALSL |
| KEGG ID | rso:RSp0743 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 8.3e-61 | 12..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-170 | MAKKESVQKRLQKVRPPRVQLTYDVEKGDAIEQKELPFVVGVVADLSGQSEVAQPKLRDRKFVNIDRDNFDDVMSAAEPRAAFQVQNTLTEEGGRFGVDLKFRSLEDFSPEAVVNQIEPLRQLLEARSKLADLRNKLAGNDKLEDLLSEVLNNTEALQKLGVKTDGGEDA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9955 | - | - |
Protein sequence: 171 a.a.
>T6CP000203 NC_003296:933060-933572 [Ralstonia solanacearum GMI1000] [TssB]
MAKKESVQKRLQKVRPPRVQLTYDVEKGDAIEQKELPFVVGVVADLSGQSEVAQPKLRDRKFVNIDRDNFDDVMSAAEPR
AAFQVQNTLTEEGGRFGVDLKFRSLEDFSPEAVVNQIEPLRQLLEARSKLADLRNKLAGNDKLEDLLSEVLNNTEALQKL
GVKTDGGEDA*
MAKKESVQKRLQKVRPPRVQLTYDVEKGDAIEQKELPFVVGVVADLSGQSEVAQPKLRDRKFVNIDRDNFDDVMSAAEPR
AAFQVQNTLTEEGGRFGVDLKFRSLEDFSPEAVVNQIEPLRQLLEARSKLADLRNKLAGNDKLEDLLSEVLNNTEALQKL
GVKTDGGEDA*
Nucleotide sequence: 513 bp
>T6CP000203 NC_003296:933060-933572 [Ralstonia solanacearum GMI1000] [TssB]
ATGGCTAAGAAAGAAAGTGTGCAGAAGCGTCTGCAGAAGGTGCGCCCCCCGCGCGTGCAGCTGACGTACGACGTTGAAAA
GGGCGACGCGATCGAGCAGAAGGAACTGCCGTTCGTGGTCGGCGTGGTGGCGGACCTGTCGGGCCAGTCGGAAGTGGCGC
AGCCCAAGCTGCGCGACCGCAAGTTCGTCAACATCGACCGCGACAACTTCGACGACGTGATGTCGGCGGCCGAGCCGCGC
GCTGCCTTTCAGGTGCAGAACACGCTGACGGAAGAGGGCGGCCGCTTCGGCGTCGACCTCAAGTTCCGCTCGCTGGAGGA
TTTCTCGCCGGAAGCCGTGGTGAACCAGATCGAGCCGCTGCGCCAGCTGCTGGAGGCACGCTCCAAGCTGGCCGACCTGC
GCAACAAGCTGGCCGGCAACGACAAGCTGGAAGATCTGCTGTCCGAGGTGCTGAACAACACCGAGGCCCTGCAGAAGCTG
GGTGTCAAGACGGATGGGGGTGAGGATGCTTGA
ATGGCTAAGAAAGAAAGTGTGCAGAAGCGTCTGCAGAAGGTGCGCCCCCCGCGCGTGCAGCTGACGTACGACGTTGAAAA
GGGCGACGCGATCGAGCAGAAGGAACTGCCGTTCGTGGTCGGCGTGGTGGCGGACCTGTCGGGCCAGTCGGAAGTGGCGC
AGCCCAAGCTGCGCGACCGCAAGTTCGTCAACATCGACCGCGACAACTTCGACGACGTGATGTCGGCGGCCGAGCCGCGC
GCTGCCTTTCAGGTGCAGAACACGCTGACGGAAGAGGGCGGCCGCTTCGGCGTCGACCTCAAGTTCCGCTCGCTGGAGGA
TTTCTCGCCGGAAGCCGTGGTGAACCAGATCGAGCCGCTGCGCCAGCTGCTGGAGGCACGCTCCAAGCTGGCCGACCTGC
GCAACAAGCTGGCCGGCAACGACAAGCTGGAAGATCTGCTGTCCGAGGTGCTGAACAACACCGAGGCCCTGCAGAAGCTG
GGTGTCAAGACGGATGGGGGTGAGGATGCTTGA