Detailed information of component protein


Summary

Component ID T6CP011416
Component protein type TagL
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (278 a.a.); Nucleotide sequence (834 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20760
Coordinate (Strand) 959306..960139 (-)
NCBI ID WP_011004059.1
RefSeq NC_003296
Uniprot ID M4UP53_RALSL, A0A223GUQ7_9RALS, Q8XRS0_RALSO, A0A0K1ZTZ0_RALSL
KEGG ID rse:F504_4457, rso:RSp0761
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
OmpA PF00691.22 2.3e-22 175..269
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-277 MGLDAIRYGFRPATVLAAALAAACLGTLPAHAAPGDPAPASATSPVPGPVVAGGMVPDEATKASVLARLRELYGSANVVDQIEVGNVVSPPNWTGNVQKILSPQIKQVSRGQLNIDGTQVAVHGDVQNEAQRQQLASDMATTLGASYTIKNGLRVAAVSEQSLLDQTLANRIIEFETGSATLTPKGRGILDEMAAVLPRLNGRKIEIVGHTDNSGSRAMNLNLSQARAETVKNYLISKGAEPGTLTAIGVGPDQPVAPNATDEGRSRNRRIEFRASQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.6539 pos: 32-33. AHA-AP. MGLDAIRYGFRPATVLAAALAAACLGTLPAHA


  Protein sequence: 278 a.a.    

>T6CP011416 NC_003296:c960139-959306 [Ralstonia solanacearum GMI1000] [TagL]
MGLDAIRYGFRPATVLAAALAAACLGTLPAHAAPGDPAPASATSPVPGPVVAGGMVPDEATKASVLARLRELYGSANVVD
QIEVGNVVSPPNWTGNVQKILSPQIKQVSRGQLNIDGTQVAVHGDVQNEAQRQQLASDMATTLGASYTIKNGLRVAAVSE
QSLLDQTLANRIIEFETGSATLTPKGRGILDEMAAVLPRLNGRKIEIVGHTDNSGSRAMNLNLSQARAETVKNYLISKGA
EPGTLTAIGVGPDQPVAPNATDEGRSRNRRIEFRASQ*

  Nucleotide sequence: 834 bp    

>T6CP011416 NC_003296:c960139-959306 [Ralstonia solanacearum GMI1000] [TagL]
ATGGGTCTGGATGCAATCAGGTACGGCTTCCGGCCTGCAACGGTACTGGCGGCGGCCCTCGCGGCGGCTTGCCTGGGCAC
CCTGCCCGCTCACGCCGCGCCGGGCGACCCGGCCCCCGCGTCCGCAACCAGCCCCGTGCCGGGGCCGGTCGTGGCCGGCG
GCATGGTTCCGGACGAGGCCACCAAGGCGAGCGTGCTGGCCAGGCTGCGCGAGCTCTACGGCAGTGCCAACGTGGTCGAC
CAGATCGAGGTCGGCAACGTGGTGTCGCCGCCCAACTGGACGGGCAACGTGCAGAAGATCCTGTCGCCGCAGATCAAGCA
GGTCAGCCGCGGCCAGCTCAACATCGACGGCACGCAGGTGGCCGTGCACGGCGACGTGCAAAACGAAGCACAGCGCCAGC
AGCTCGCCAGCGACATGGCCACCACGCTCGGCGCCAGCTACACGATCAAGAACGGACTGCGCGTGGCCGCCGTCTCGGAA
CAGAGCCTGCTGGACCAGACCCTGGCCAACCGCATCATCGAGTTCGAGACCGGCAGCGCCACGCTCACGCCCAAGGGCCG
CGGCATCCTGGATGAAATGGCCGCCGTGCTGCCGCGCCTGAACGGCCGCAAGATCGAGATCGTCGGCCACACCGACAACT
CGGGCAGCCGCGCCATGAACCTCAACCTGAGCCAGGCCCGCGCCGAGACGGTGAAGAACTACCTGATCAGCAAGGGCGCC
GAACCCGGCACGCTCACCGCCATCGGCGTCGGGCCGGACCAGCCGGTGGCACCCAACGCCACCGACGAGGGGCGGTCGCG
CAATCGGCGGATCGAGTTCCGGGCTAGCCAGTGA