Detailed information of component protein


Summary

Component ID T6CP000207
Component protein type TssF
T6SS ID (Type) T6SS00026 experimental (Type i4b)
Strain Ralstonia solanacearum GMI1000
Replicon plasmid pGMI1000MP
Sequence Protein sequence (617 a.a.); Nucleotide sequence (1851 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) RS_RS20690
Coordinate (Strand) 936244..938094 (+)
NCBI ID WP_011004045.1
RefSeq NC_003296
Uniprot ID Q8XRT4_RALSO
KEGG ID rso:RSp0747
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 5e-191 2..612
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-616 MEELLPYYERELAFLRRYSRDFAERYPKIAGRLAMSGDGCDDPHVERMIESFALLSARVSKKLDDDYPEFTEALLEVLYPHYLRPFPSCSVVHFDVRGAAAQLSAPVQIPRGTFLTSRVVRKVECSFRTAYDVTFAPVRIAAATYARAVSAPSAVSLPKGATGAIALTLEYVGERGGFESLPLDRLRVYMDGEPSFVAALGDALFLHTAGTYVEGERPGVWTGLSGVPLHEVGFAEDEALIDCPARSHPAYRLLTEHFSFAEKFNFFDIDLAAVRRRLTPGVPVRRLVLHLVLKDVRSDSHAARLLEGLQAEHLRLHCTPVVNLFQQNADPIRIEHTASAYPVVADSRRAHGFDIYSIDKVHLVRETAAHEEVTEFRPFYSLHHGEEPGQAGHYWIARRNELVAQRSPGFETEISIVDTRFDPASPQTDTLSLTVTCTNRDLPSTLAFGQPDGDLSMEGNSVARAIMLLRKPTPSMRLGGGRAAQWRLISMLSLNHLSLVQNGLPTLKEMLRLHDLPHSAISARQIEGVVGLDYAPATHWLAGKPFATFVRGLEIRLTLDEDAFVGTGLHRFIRVLDHFFGLYVHINSFVQLIAVSRRSGKELVRCAPRSGESILV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9896 - -


  Protein sequence: 617 a.a.    

>T6CP000207 NC_003296:936244-938094 [Ralstonia solanacearum GMI1000] [TssF]
MEELLPYYERELAFLRRYSRDFAERYPKIAGRLAMSGDGCDDPHVERMIESFALLSARVSKKLDDDYPEFTEALLEVLYP
HYLRPFPSCSVVHFDVRGAAAQLSAPVQIPRGTFLTSRVVRKVECSFRTAYDVTFAPVRIAAATYARAVSAPSAVSLPKG
ATGAIALTLEYVGERGGFESLPLDRLRVYMDGEPSFVAALGDALFLHTAGTYVEGERPGVWTGLSGVPLHEVGFAEDEAL
IDCPARSHPAYRLLTEHFSFAEKFNFFDIDLAAVRRRLTPGVPVRRLVLHLVLKDVRSDSHAARLLEGLQAEHLRLHCTP
VVNLFQQNADPIRIEHTASAYPVVADSRRAHGFDIYSIDKVHLVRETAAHEEVTEFRPFYSLHHGEEPGQAGHYWIARRN
ELVAQRSPGFETEISIVDTRFDPASPQTDTLSLTVTCTNRDLPSTLAFGQPDGDLSMEGNSVARAIMLLRKPTPSMRLGG
GRAAQWRLISMLSLNHLSLVQNGLPTLKEMLRLHDLPHSAISARQIEGVVGLDYAPATHWLAGKPFATFVRGLEIRLTLD
EDAFVGTGLHRFIRVLDHFFGLYVHINSFVQLIAVSRRSGKELVRCAPRSGESILV*

  Nucleotide sequence: 1851 bp    

>T6CP000207 NC_003296:936244-938094 [Ralstonia solanacearum GMI1000] [TssF]
ATGGAAGAGCTGCTGCCCTATTACGAACGAGAACTGGCCTTCCTGCGCCGGTACTCGCGCGATTTCGCCGAGCGTTATCC
GAAGATCGCTGGCCGGCTCGCCATGTCGGGCGACGGCTGCGACGATCCGCACGTCGAGCGGATGATCGAGTCGTTCGCGC
TGCTGAGCGCCCGCGTCAGCAAGAAGCTGGACGACGACTACCCCGAATTCACCGAGGCGCTGCTGGAGGTCCTGTACCCC
CATTACCTGCGTCCGTTCCCGTCGTGCTCCGTGGTGCATTTCGATGTGCGCGGCGCGGCGGCCCAGCTCAGCGCGCCGGT
GCAGATTCCGCGTGGCACCTTCCTGACCTCGCGCGTGGTGCGCAAGGTCGAGTGCAGTTTCCGCACCGCTTACGACGTCA
CCTTCGCGCCGGTGCGGATTGCCGCGGCCACTTACGCGCGCGCCGTGTCCGCGCCGTCGGCGGTCAGTCTGCCCAAGGGG
GCGACCGGTGCGATCGCGCTGACCCTGGAATACGTTGGCGAGCGCGGCGGCTTCGAGTCCCTGCCGCTCGACCGGCTGCG
CGTCTACATGGACGGCGAGCCCTCGTTCGTGGCCGCGCTGGGGGATGCGCTGTTCCTGCATACCGCGGGAACCTACGTGG
AGGGCGAGCGACCGGGCGTGTGGACCGGCTTGTCCGGTGTGCCCCTGCATGAGGTCGGCTTCGCGGAAGACGAGGCGCTG
ATCGACTGCCCGGCGCGCTCGCATCCGGCCTATCGGTTGCTGACCGAGCACTTCAGTTTTGCCGAGAAATTCAATTTCTT
CGACATCGACCTGGCAGCCGTGCGGCGCAGGCTGACGCCCGGCGTGCCGGTTCGCCGCCTGGTGCTGCACCTGGTCCTGA
AGGACGTGCGCAGCGACTCGCACGCTGCCCGCCTGCTCGAAGGCTTGCAGGCCGAACACCTGCGCCTGCATTGCACGCCG
GTGGTCAACCTGTTCCAGCAGAATGCCGATCCGATCCGCATCGAGCACACCGCCAGCGCCTACCCGGTCGTGGCCGACAG
CCGACGTGCGCACGGCTTCGACATCTACTCGATCGACAAGGTGCACCTGGTGCGCGAGACCGCCGCGCACGAAGAGGTGA
CCGAGTTCCGCCCGTTCTACTCGCTGCACCACGGCGAGGAGCCCGGGCAAGCGGGCCACTACTGGATCGCCCGGCGCAAC
GAGCTCGTGGCGCAGCGCAGCCCCGGCTTCGAGACTGAGATCTCCATCGTCGATACCCGGTTCGATCCGGCCAGCCCGCA
GACCGACACGCTGAGCCTGACCGTCACCTGCACCAACCGCGATCTGCCGTCCACGCTGGCGTTCGGCCAGCCGGACGGCG
ACCTGTCGATGGAAGGCAATTCCGTCGCTCGCGCCATCATGTTGCTGCGCAAGCCGACGCCGTCGATGCGCCTGGGCGGC
GGCCGCGCGGCGCAGTGGCGGCTGATTTCGATGCTCTCGCTCAACCACCTGTCGCTGGTGCAGAACGGGCTGCCGACGCT
CAAGGAGATGCTGCGCCTGCATGATCTGCCGCATAGCGCCATTTCGGCGCGGCAGATCGAGGGCGTGGTCGGCCTCGACT
ACGCGCCGGCCACGCACTGGCTGGCCGGCAAGCCGTTCGCCACGTTTGTGCGGGGGCTGGAGATCCGGCTCACGCTGGAC
GAAGACGCTTTCGTCGGCACGGGCCTGCATCGCTTCATCCGCGTGCTGGACCACTTCTTCGGCCTGTACGTCCATATCAA
CAGCTTTGTGCAGCTGATTGCGGTATCGCGCCGCAGCGGCAAGGAACTGGTCAGATGCGCACCTCGCAGCGGCGAATCGA
TCCTGGTGTGA