Detailed information of component protein
Summary
| Component ID | T6CP000206 |
| Component protein type | TssE |
| T6SS ID (Type) | T6SS00026  (Type i4b) |
| Strain | Ralstonia solanacearum GMI1000 |
| Replicon | plasmid pGMI1000MP |
| Sequence | Protein sequence (158 a.a.); Nucleotide sequence (474 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | RS_RS20685 |
| Coordinate (Strand) | 935708..936181 (+) |
| NCBI ID | WP_011004044.1 |
| RefSeq | NC_003296 |
| Uniprot ID | A0A223GUI9_9RALS, Q8XRT5_RALSO, A0A0S4V1C5_RALSL |
| KEGG ID | rso:RSp0746 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GPW_gp25 | PF04965.16 | 8.8e-20 | 32..132 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-157 | MKGFEPSLLDKLFDDEPHAPLPTALRQLSLEELKSTVARDIESLLNTRIVFEEDDLQSLPECKRSLLTYGLNDFAGLSLASFYDRNYICQSLTKAIERHEPRLQHVSVSLEINERATNALCFGITAVLLVGPAREPVSFDAMLQPSTLRYSVSRGRG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9934 | - | - |
Protein sequence: 158 a.a.
>T6CP000206 NC_003296:935708-936181 [Ralstonia solanacearum GMI1000] [TssE]
MKGFEPSLLDKLFDDEPHAPLPTALRQLSLEELKSTVARDIESLLNTRIVFEEDDLQSLPECKRSLLTYGLNDFAGLSLA
SFYDRNYICQSLTKAIERHEPRLQHVSVSLEINERATNALCFGITAVLLVGPAREPVSFDAMLQPSTLRYSVSRGRG*
MKGFEPSLLDKLFDDEPHAPLPTALRQLSLEELKSTVARDIESLLNTRIVFEEDDLQSLPECKRSLLTYGLNDFAGLSLA
SFYDRNYICQSLTKAIERHEPRLQHVSVSLEINERATNALCFGITAVLLVGPAREPVSFDAMLQPSTLRYSVSRGRG*
Nucleotide sequence: 474 bp
>T6CP000206 NC_003296:935708-936181 [Ralstonia solanacearum GMI1000] [TssE]
ATGAAAGGTTTCGAACCCAGCCTGCTCGATAAGCTCTTCGACGACGAGCCGCATGCGCCCTTGCCGACGGCTCTGCGGCA
GCTTTCGCTGGAAGAGCTCAAGAGCACGGTGGCGCGCGATATCGAGTCCTTGCTCAATACGCGCATCGTCTTTGAAGAGG
ACGACCTGCAGAGCCTGCCCGAATGCAAGCGGTCGCTGCTGACCTACGGGCTGAACGATTTCGCGGGCCTGAGCCTCGCC
AGCTTCTACGACCGCAATTACATCTGCCAGTCGCTCACCAAGGCGATCGAGCGGCACGAGCCGCGGCTGCAGCACGTCAG
TGTCTCGCTTGAAATCAACGAGCGGGCGACCAACGCGCTGTGCTTCGGCATCACTGCGGTCTTGCTGGTCGGGCCGGCGC
GCGAGCCCGTGAGCTTTGATGCGATGCTGCAGCCGTCCACGCTGCGGTATTCCGTCTCGCGCGGGCGCGGCTGA
ATGAAAGGTTTCGAACCCAGCCTGCTCGATAAGCTCTTCGACGACGAGCCGCATGCGCCCTTGCCGACGGCTCTGCGGCA
GCTTTCGCTGGAAGAGCTCAAGAGCACGGTGGCGCGCGATATCGAGTCCTTGCTCAATACGCGCATCGTCTTTGAAGAGG
ACGACCTGCAGAGCCTGCCCGAATGCAAGCGGTCGCTGCTGACCTACGGGCTGAACGATTTCGCGGGCCTGAGCCTCGCC
AGCTTCTACGACCGCAATTACATCTGCCAGTCGCTCACCAAGGCGATCGAGCGGCACGAGCCGCGGCTGCAGCACGTCAG
TGTCTCGCTTGAAATCAACGAGCGGGCGACCAACGCGCTGTGCTTCGGCATCACTGCGGTCTTGCTGGTCGGGCCGGCGC
GCGAGCCCGTGAGCTTTGATGCGATGCTGCAGCCGTCCACGCTGCGGTATTCCGTCTCGCGCGGGCGCGGCTGA