Detailed information of component protein
Summary
| Component ID | T6CP000202 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00026  (Type i4b) |
| Strain | Ralstonia solanacearum GMI1000 |
| Replicon | plasmid pGMI1000MP |
| Sequence | Protein sequence (225 a.a.); Nucleotide sequence (675 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | RS_RS20660 |
| Coordinate (Strand) | 931427..932101 (-) |
| NCBI ID | WP_028854260.1 |
| RefSeq | NC_003296 |
| Uniprot ID | A0A223GUP0_9RALS, A0A0K1ZRT8_RALSL |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 2.5e-30 | 74..192 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-27 | MKNIEFKATTRLPGCGSRRRIATGLQR |
| Transmembrane helix | 28-50 | AVAGMAVIALTACSTGASVLAGA |
| Outside | 51-224 | ASGALEAVGLKASNVPDAQKPPREVPLKLTAGANLNAATDRRPVALVVRLYALKDPTSFLQAPYDTFIDPAREKQTLGADLVQVREMTLIPGQRYEFSEKVSREASTLGVVALFRSPAAQRWKFAFNAEKNEKSGIMIGLHACAMTLTTGSPTTPAGSAPLSDLNLLSPASCGG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| TAT(Tat/SPI) | 0.4203 | pos: 48-49. VLA-GA. | MKNIEFKATTRLPGCGSRRRIATGLQRAVAGMAVIALTACSTGASVLA |
Protein sequence: 225 a.a.
>T6CP000202 NC_003296:c932101-931427 [Ralstonia solanacearum GMI1000] [TssJ]
MKNIEFKATTRLPGCGSRRRIATGLQRAVAGMAVIALTACSTGASVLAGAASGALEAVGLKASNVPDAQKPPREVPLKLT
AGANLNAATDRRPVALVVRLYALKDPTSFLQAPYDTFIDPAREKQTLGADLVQVREMTLIPGQRYEFSEKVSREASTLGV
VALFRSPAAQRWKFAFNAEKNEKSGIMIGLHACAMTLTTGSPTTPAGSAPLSDLNLLSPASCGG*
MKNIEFKATTRLPGCGSRRRIATGLQRAVAGMAVIALTACSTGASVLAGAASGALEAVGLKASNVPDAQKPPREVPLKLT
AGANLNAATDRRPVALVVRLYALKDPTSFLQAPYDTFIDPAREKQTLGADLVQVREMTLIPGQRYEFSEKVSREASTLGV
VALFRSPAAQRWKFAFNAEKNEKSGIMIGLHACAMTLTTGSPTTPAGSAPLSDLNLLSPASCGG*
Nucleotide sequence: 675 bp
>T6CP000202 NC_003296:c932101-931427 [Ralstonia solanacearum GMI1000] [TssJ]
ATGAAGAATATTGAATTCAAGGCTACGACAAGGCTGCCGGGCTGCGGCAGCCGGCGCCGCATCGCGACCGGGCTCCAGCG
GGCAGTCGCGGGGATGGCCGTGATCGCCCTGACCGCCTGCTCGACCGGCGCCAGCGTGCTGGCGGGCGCCGCCAGTGGCG
CGCTGGAGGCCGTGGGGCTGAAGGCATCGAACGTGCCGGACGCGCAGAAACCGCCCCGCGAGGTTCCGCTCAAGCTGACC
GCCGGCGCCAACCTGAACGCGGCGACGGACCGCCGCCCGGTCGCGCTGGTGGTCCGGTTATACGCACTCAAGGATCCGAC
CAGTTTCCTGCAGGCGCCGTATGACACGTTTATTGATCCGGCGCGTGAAAAACAAACGCTCGGCGCCGATCTGGTCCAGG
TCCGGGAAATGACGCTGATTCCCGGCCAGCGCTACGAATTCTCGGAAAAAGTCTCGCGTGAAGCCAGTACACTGGGCGTT
GTTGCACTATTCCGCAGTCCGGCCGCCCAACGATGGAAATTCGCTTTTAATGCGGAAAAGAATGAGAAAAGCGGCATCAT
GATCGGATTGCACGCTTGCGCGATGACCCTGACCACGGGCTCGCCGACCACCCCCGCCGGCAGCGCGCCGCTGTCCGACC
TGAACCTGCTTTCGCCGGCAAGCTGCGGCGGCTGA
ATGAAGAATATTGAATTCAAGGCTACGACAAGGCTGCCGGGCTGCGGCAGCCGGCGCCGCATCGCGACCGGGCTCCAGCG
GGCAGTCGCGGGGATGGCCGTGATCGCCCTGACCGCCTGCTCGACCGGCGCCAGCGTGCTGGCGGGCGCCGCCAGTGGCG
CGCTGGAGGCCGTGGGGCTGAAGGCATCGAACGTGCCGGACGCGCAGAAACCGCCCCGCGAGGTTCCGCTCAAGCTGACC
GCCGGCGCCAACCTGAACGCGGCGACGGACCGCCGCCCGGTCGCGCTGGTGGTCCGGTTATACGCACTCAAGGATCCGAC
CAGTTTCCTGCAGGCGCCGTATGACACGTTTATTGATCCGGCGCGTGAAAAACAAACGCTCGGCGCCGATCTGGTCCAGG
TCCGGGAAATGACGCTGATTCCCGGCCAGCGCTACGAATTCTCGGAAAAAGTCTCGCGTGAAGCCAGTACACTGGGCGTT
GTTGCACTATTCCGCAGTCCGGCCGCCCAACGATGGAAATTCGCTTTTAATGCGGAAAAGAATGAGAAAAGCGGCATCAT
GATCGGATTGCACGCTTGCGCGATGACCCTGACCACGGGCTCGCCGACCACCCCCGCCGGCAGCGCGCCGCTGTCCGACC
TGAACCTGCTTTCGCCGGCAAGCTGCGGCGGCTGA