Detailed information of component protein
Summary
| Component ID | T6CP000205 |
| Component protein type | TssD |
| T6SS ID (Type) | T6SS00026  (Type i4b) |
| Strain | Ralstonia solanacearum GMI1000 |
| Replicon | plasmid pGMI1000MP |
| Sequence | Protein sequence (168 a.a.); Nucleotide sequence (504 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | RS_RS20680 |
| Coordinate (Strand) | 935144..935647 (+) |
| NCBI ID | WP_011004043.1 |
| RefSeq | NC_003296 |
| Uniprot ID | M4V2U4_RALSL, A0A223GUI7_9RALS, Q8XRT6_RALSO, A0A0K1ZS84_RALSL |
| KEGG ID | rse:F504_4476, rso:RSp0745 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 6.5e-37 | 5..139 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-167 | MKDIYVKFDSPAIKGESQDKDHKEWIEVNSWSQSIIQPRSATASTAGGHTAERCEHRDMVFSKDLDVVSPLLYQHASGGTTFGEVTIEFFRADGEGNRVKYLEVKLKNAILSEVDSQVAAEGIPTDTFSLRYAAVQWKYTQQKSAGGQGGNSQGAWSLTKNDKTYSV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9747 | - | - |
Protein sequence: 168 a.a.
>T6CP000205 NC_003296:935144-935647 [Ralstonia solanacearum GMI1000] [TssD]
MKDIYVKFDSPAIKGESQDKDHKEWIEVNSWSQSIIQPRSATASTAGGHTAERCEHRDMVFSKDLDVVSPLLYQHASGGT
TFGEVTIEFFRADGEGNRVKYLEVKLKNAILSEVDSQVAAEGIPTDTFSLRYAAVQWKYTQQKSAGGQGGNSQGAWSLTK
NDKTYSV*
MKDIYVKFDSPAIKGESQDKDHKEWIEVNSWSQSIIQPRSATASTAGGHTAERCEHRDMVFSKDLDVVSPLLYQHASGGT
TFGEVTIEFFRADGEGNRVKYLEVKLKNAILSEVDSQVAAEGIPTDTFSLRYAAVQWKYTQQKSAGGQGGNSQGAWSLTK
NDKTYSV*
Nucleotide sequence: 504 bp
>T6CP000205 NC_003296:935144-935647 [Ralstonia solanacearum GMI1000] [TssD]
ATGAAGGATATCTACGTCAAGTTCGACAGCCCGGCCATCAAGGGGGAGTCCCAGGACAAGGATCACAAGGAGTGGATCGA
AGTCAACAGCTGGTCCCAGTCGATCATCCAGCCGCGTTCGGCCACGGCCTCGACCGCGGGCGGCCATACCGCCGAGCGTT
GCGAGCACCGCGACATGGTGTTCTCCAAGGACCTGGACGTGGTCAGCCCGCTGCTGTACCAGCACGCTTCGGGCGGCACG
ACCTTCGGTGAAGTGACGATCGAGTTCTTCCGCGCCGACGGTGAAGGCAACCGCGTGAAGTACCTCGAGGTCAAGCTGAA
GAACGCGATTTTGAGCGAAGTGGATTCGCAGGTGGCTGCCGAGGGCATCCCGACCGATACGTTCAGCCTGCGCTACGCGG
CCGTGCAGTGGAAGTACACCCAGCAGAAGAGCGCCGGCGGCCAGGGCGGCAACTCGCAGGGCGCCTGGAGCCTGACCAAG
AACGACAAGACCTACTCGGTTTAA
ATGAAGGATATCTACGTCAAGTTCGACAGCCCGGCCATCAAGGGGGAGTCCCAGGACAAGGATCACAAGGAGTGGATCGA
AGTCAACAGCTGGTCCCAGTCGATCATCCAGCCGCGTTCGGCCACGGCCTCGACCGCGGGCGGCCATACCGCCGAGCGTT
GCGAGCACCGCGACATGGTGTTCTCCAAGGACCTGGACGTGGTCAGCCCGCTGCTGTACCAGCACGCTTCGGGCGGCACG
ACCTTCGGTGAAGTGACGATCGAGTTCTTCCGCGCCGACGGTGAAGGCAACCGCGTGAAGTACCTCGAGGTCAAGCTGAA
GAACGCGATTTTGAGCGAAGTGGATTCGCAGGTGGCTGCCGAGGGCATCCCGACCGATACGTTCAGCCTGCGCTACGCGG
CCGTGCAGTGGAAGTACACCCAGCAGAAGAGCGCCGGCGGCCAGGGCGGCAACTCGCAGGGCGCCTGGAGCCTGACCAAG
AACGACAAGACCTACTCGGTTTAA