Detailed information of component protein
Summary
| Component ID | T6CP000200 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS00026  (Type i4b) |
| Strain | Ralstonia solanacearum GMI1000 |
| Replicon | plasmid pGMI1000MP |
| Sequence | Protein sequence (264 a.a.); Nucleotide sequence (792 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | RS_RS20650 |
| Coordinate (Strand) | 929249..930040 (-) |
| NCBI ID | WP_011004037.1 |
| RefSeq | NC_003296 |
| Uniprot ID | Q8XRU2_RALSO |
| KEGG ID | rso:RSp0739 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 5.9e-58 | 34..231 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-211 | MSATTTPSLFGGSAAPAGNAAADARESNTHVRTLLDLLYDGFFMLFQLRNGQQPTSAEDFLTRVRAFLEDFDRGAKRLNVSAEDVFDAKYAFCAAVDETILSSNFSIRTAWERRPLQLELFGEQLAGETFFIKLEELRAHGAPRLQALEVFHMCLLLGFRGKYILEGPEKLAYLTARLGDEISAIKGKRAAFAPHWLLPDKVSHRLKRETP |
| Transmembrane helix | 212-234 | LWIFGAVFALIALLGYIGLSSTL |
| Inside | 235-263 | RAKTNETLEGYSQVIKLGPRFSHLTISLP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.7008 | - | - |
Protein sequence: 264 a.a.
>T6CP000200 NC_003296:c930040-929249 [Ralstonia solanacearum GMI1000] [TssL]
MSATTTPSLFGGSAAPAGNAAADARESNTHVRTLLDLLYDGFFMLFQLRNGQQPTSAEDFLTRVRAFLEDFDRGAKRLNV
SAEDVFDAKYAFCAAVDETILSSNFSIRTAWERRPLQLELFGEQLAGETFFIKLEELRAHGAPRLQALEVFHMCLLLGFR
GKYILEGPEKLAYLTARLGDEISAIKGKRAAFAPHWLLPDKVSHRLKRETPLWIFGAVFALIALLGYIGLSSTLRAKTNE
TLEGYSQVIKLGPRFSHLTISLP*
MSATTTPSLFGGSAAPAGNAAADARESNTHVRTLLDLLYDGFFMLFQLRNGQQPTSAEDFLTRVRAFLEDFDRGAKRLNV
SAEDVFDAKYAFCAAVDETILSSNFSIRTAWERRPLQLELFGEQLAGETFFIKLEELRAHGAPRLQALEVFHMCLLLGFR
GKYILEGPEKLAYLTARLGDEISAIKGKRAAFAPHWLLPDKVSHRLKRETPLWIFGAVFALIALLGYIGLSSTLRAKTNE
TLEGYSQVIKLGPRFSHLTISLP*
Nucleotide sequence: 792 bp
>T6CP000200 NC_003296:c930040-929249 [Ralstonia solanacearum GMI1000] [TssL]
ATGAGCGCTACCACCACGCCTTCGCTGTTCGGCGGCTCGGCCGCCCCCGCCGGCAATGCCGCAGCCGATGCGCGCGAATC
CAACACGCACGTGCGCACGCTGCTGGACCTGCTCTACGATGGCTTCTTCATGCTCTTCCAGCTGCGCAACGGCCAGCAGC
CGACCAGCGCGGAAGACTTCCTCACCCGCGTGCGCGCCTTCCTGGAAGATTTCGACCGCGGCGCCAAGCGGCTGAACGTG
TCGGCCGAAGACGTGTTCGACGCCAAGTACGCGTTCTGCGCCGCCGTGGACGAGACCATCCTGTCGTCCAACTTCTCCAT
CCGTACCGCCTGGGAGCGCCGTCCGCTGCAGCTGGAGCTGTTCGGCGAGCAGCTCGCCGGTGAAACGTTCTTCATCAAGC
TGGAAGAGCTGCGTGCCCACGGCGCGCCGCGCCTGCAGGCCCTGGAGGTCTTCCACATGTGCCTGCTGCTGGGCTTCCGC
GGCAAGTACATCCTCGAAGGGCCGGAGAAGCTGGCCTACCTGACCGCCCGCCTGGGCGACGAGATCTCCGCCATCAAGGG
CAAGCGCGCGGCCTTCGCGCCGCACTGGCTGCTGCCGGACAAGGTGTCGCACCGGCTCAAGCGCGAAACGCCGCTGTGGA
TCTTCGGCGCGGTCTTCGCGCTGATCGCGCTGCTGGGGTACATCGGCCTGTCGTCCACGCTGCGCGCGAAGACGAACGAA
ACGCTGGAAGGCTATTCGCAGGTCATCAAGCTCGGGCCGCGCTTCTCGCACCTGACCATCTCGCTGCCCTGA
ATGAGCGCTACCACCACGCCTTCGCTGTTCGGCGGCTCGGCCGCCCCCGCCGGCAATGCCGCAGCCGATGCGCGCGAATC
CAACACGCACGTGCGCACGCTGCTGGACCTGCTCTACGATGGCTTCTTCATGCTCTTCCAGCTGCGCAACGGCCAGCAGC
CGACCAGCGCGGAAGACTTCCTCACCCGCGTGCGCGCCTTCCTGGAAGATTTCGACCGCGGCGCCAAGCGGCTGAACGTG
TCGGCCGAAGACGTGTTCGACGCCAAGTACGCGTTCTGCGCCGCCGTGGACGAGACCATCCTGTCGTCCAACTTCTCCAT
CCGTACCGCCTGGGAGCGCCGTCCGCTGCAGCTGGAGCTGTTCGGCGAGCAGCTCGCCGGTGAAACGTTCTTCATCAAGC
TGGAAGAGCTGCGTGCCCACGGCGCGCCGCGCCTGCAGGCCCTGGAGGTCTTCCACATGTGCCTGCTGCTGGGCTTCCGC
GGCAAGTACATCCTCGAAGGGCCGGAGAAGCTGGCCTACCTGACCGCCCGCCTGGGCGACGAGATCTCCGCCATCAAGGG
CAAGCGCGCGGCCTTCGCGCCGCACTGGCTGCTGCCGGACAAGGTGTCGCACCGGCTCAAGCGCGAAACGCCGCTGTGGA
TCTTCGGCGCGGTCTTCGCGCTGATCGCGCTGCTGGGGTACATCGGCCTGTCGTCCACGCTGCGCGCGAAGACGAACGAA
ACGCTGGAAGGCTATTCGCAGGTCATCAAGCTCGGGCCGCGCTTCTCGCACCTGACCATCTCGCTGCCCTGA