Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
T6SS ID | T6SS00002 |
T6SS type | Type i1 |
Strain | Vibrio cholerae O1 biovar El Tor str. N16961 |
Replicon (RefSeq) | chromosome II (Vas) (NC_002506) |
Location | 110871..145577 |
Function | - |
Comment | T6SS introduces cytotoxicity towards Dictyostelium and cultured mammalian macrophages. T6SS is involved in inflammatory response, diarrhoea and actin cross-linking in mouse intestine. T6SS leads to killing of E. coli, Salmonella and Citrobacter rodentium. |
References | [1] Dong TG et al (2012) Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52. Nucleic Acids Res. 40(16):7766-75. [PudMed:22723378] [2] Kitaoka M et al (2011) Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol. 60(Pt 4):397-407. [PudMed:21252269] [3] Pietrosiuk A et al (2011) Molecular basis for the unique role of the AAA+ chaperone ClpV in type VI protein secretion. J Biol Chem. 286(34):30010-21. [PudMed:21733841] [4] Altindis E et al (2015) Secretome Analysis of Vibrio cholerae Type VI Secretion System Reveals a New Effector-Immunity Pair. MBio. pii: e00075-15. [PudMed:25759499] [5] Ishikawa T et al (2012) Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. Infect Immun. 80(2):575-84. [PudMed:22083711] [6] Zheng J et al (2011) Genetic analysis of anti-amoebae and anti-bacterial activities of the type VI secretion system in Vibrio cholerae. PLoS One. 6(8):e23876. [PudMed:21909372] [7] Pukatzki S et al (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199] [8] Borgeaud S et al (2015) Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784] [9] Dong TG et al (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380] [10] Basler M et al (2012) Type VI secretion requires a dynamic contractile phage tail-like structure. Nature. 483(7388):182-6. [PudMed:22367545] [11] Pukatzki S et al (2007) Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc Natl Acad Sci U S A. 104(39):15508-13. [PudMed:17873062] [12] Miyata ST et al (2011) Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399] [13] Russell AB et al (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891] [14] Yang X et al (2014) Molecular mechanism for self-protection against the type VI secretion system in Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 70(Pt 4):1094-103. [PudMed:24699653] [15] Kitaoka M et al (2011) VasH is a transcriptional regulator of the type VI secretion system functional in endemic and pandemic Vibrio cholerae. J Bacteriol. 193(23):6471-82. [PudMed:21949076] [16] Miyata ST et al (2013) Dual Expression Profile of Type VI Secretion System Immunity Genes Protects Pandemic Vibrio cholerae. PLoS Pathog. 9(12):e1003752. [PudMed:24348240] [17] Records AR et al (2011) The type VI secretion system: a multipurpose delivery system with a phage-like machinery. Mol Plant Microbe Interact. 24(7):751-7. [PudMed:21361789] [18] Ma AT et al (2009) Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133] [19] Shao Y et al (2014) Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180] [20] Unterweger D et al (2012) Constitutive type VI secretion system expression gives Vibrio cholerae intra- and interspecific competitive advantages. PLoS One. 7(10):e48320. [PudMed:23110230] [21] MacIntyre DL et al (2010) The Vibrio cholerae type VI secretion system displays antimicrobial properties. Proc Natl Acad Sci U S A. 107(45):19520-4. [PudMed:20974937] [22] Broms JE et al (2013) A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552. BMC Microbiol. 13(1):96. [PudMed:23642157] [23] Jeong JH et al (2014) Purification, crystallization and preliminary X-ray crystallographic analysis of TssL from Vibrio cholerae. Acta Crystallogr F Struct Biol Commun. 70(Pt 9):1260-3. [PudMed:25195905] |
T6SS components and genome coordinates
Component | FHA | PAAR | TssA | TssB | TssC | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | VasH |
Number | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
---|---|---|---|---|---|---|---|
VC_RS13840 | 106851..107891 | + | 1041 | WP_001134026.1 | oxidoreductase | ||
VC_RS13845 | 107928..108284 | - | 357 | WP_001249602.1 | rhodanese-like domain-containing protein | ||
VC_RS13850 | 108299..109855 | - | 1557 | WP_001882958.1 | EAL domain-containing protein | ||
VC_RS13855 | 110031..110681 | - | 651 | WP_001010777.1 | HAD family phosphatase | ||
VC_RS13860 | 110871..112649 | + | 1779 | WP_001006513.1 | SulP family inorganic anion transporter | ||
VC_RS13865 | 112691..114370 | - | 1680 | WP_001275247.1 | RluA family pseudouridine synthase | ||
T6CP011389 | PAAR | VC_RS13870 | 115142..115426 | + | 285 | WP_000526403.1 | type VI secretion system PAAR protein |
VC_RS13875 | 115494..116414 | + | 921 | WP_001901866.1 | sel1 repeat family protein | ||
T6CP000001 | TssB | VC_RS13880 (tssB) | 116846..117352 | + | 507 | WP_000031391.1 | type VI secretion system contractile sheath small subunit |
T6CP000002 | TssC | VC_RS13885 (tssC) | 117396..118871 | + | 1476 | WP_000108140.1 | type VI secretion system contractile sheath large subunit |
T6CP008374 | TssE | VC_RS13890 (tssE) | 118874..119311 | + | 438 | WP_000221000.1 | type VI secretion system baseplate subunit TssE |
T6CP000003 | TssF | VC_RS13895 (tssF) | 119317..121086 | + | 1770 | WP_000189792.1 | type VI secretion system baseplate subunit TssF |
T6CP000004 | TssG | VC_RS13900 (tssG) | 121050..122066 | + | 1017 | WP_000510181.1 | type VI secretion system baseplate subunit TssG |
T6CP011388 | FHA | VC_RS13905 (tagH) | 122069..123556 | + | 1488 | WP_001089704.1 | type VI secretion system-associated FHA domain protein TagH |
T6CP000005 | TssJ | VC_RS13910 (tssJ) | 123559..124035 | + | 477 | WP_001045311.1 | type VI secretion system lipoprotein TssJ |
T6CP000006 | TssK | VC_RS13915 (tssK) | 124042..125376 | + | 1335 | WP_000458007.1 | type VI secretion system baseplate subunit TssK |
T6CP000007 | TssL | VC_RS13920 (icmH) | 125379..126152 | + | 774 | WP_000083409.1 | type IVB secretion system protein IcmH/DotU |
T6CP000008 | TssH | VC_RS13925 (tssH) | 126178..128787 | + | 2610 | WP_000619136.1 | type VI secretion system ATPase TssH |
T6CP012413 | VasH | VC_RS13930 (vasH) | 128790..130382 | + | 1593 | WP_000084793.1 | sigma-54 dependent T6SS transcriptional regulator VasH |
VC_RS13935 (tagO) | 130379..131035 | + | 657 | WP_001223388.1 | type VI secretion system-associated protein TagO | ||
T6CP008375 | TssA | VC_RS13940 (vasJ) | 131045..132454 | + | 1410 | WP_000426093.1 | TssA family type VI secretion system protein VasJ |
T6CP000009 | TssM | VC_RS13945 (tssM) | 132515..136015 | + | 3501 | WP_000225015.1 | type VI secretion system membrane subunit TssM |
T6CP008376 | TssA | VC_RS13950 | 136066..137328 | + | 1263 | WP_000073077.1 | ImpA family type VI secretion system protein |
T6CP000010 | TssI | VC_RS13955 (tssI) | 137581..140634 | + | 3054 | WP_000113295.1 | type VI secretion system tip protein VgrG |
VC_RS13960 | 140631..140999 | + | 369 | WP_001061295.1 | hypothetical protein | ||
VC_RS13965 | 141147..141407 | - | 261 | WP_000799238.1 | DUF3012 domain-containing protein | ||
VC_RS13970 (rbsD) | 141691..142110 | + | 420 | WP_001911632.1 | D-ribose pyranase | ||
VC_RS13975 (rbsA) | 142137..143639 | + | 1503 | WP_000189455.1 | ribose ABC transporter ATP-binding protein RbsA | ||
VC_RS13980 (rbsC) | 143669..144634 | + | 966 | WP_208854928.1 | ribose ABC transporter permease | ||
VC_RS13985 (rbsB) | 144699..145577 | + | 879 | WP_000737186.1 | ribose ABC transporter substrate-binding protein RbsB | ||
VC_RS13990 (rbsK) | 145716..146636 | + | 921 | WP_001038380.1 | ribokinase | ||
VC_RS13995 | 146647..147651 | + | 1005 | WP_000224436.1 | substrate-binding domain-containing protein | ||
VC_RS14000 | 147777..148343 | + | 567 | WP_000365205.1 | nicotinamide riboside transporter PnuC | ||
VC_RS14005 | 148389..148511 | + | 123 | WP_000271123.1 | hypothetical protein | ||
VC_RS14010 | 148508..149239 | + | 732 | WP_000027380.1 | nucleoside phosphorylase | ||
VC_RS14015 | 149379..150059 | + | 681 | WP_001177523.1 | Crp/Fnr family transcriptional regulator |
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
---|---|---|---|---|---|---|---|---|
REG0039 | VC_RS13930 (VasH) | protein | Global regulator | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 128790..130382 (+) | 446006938 | VCA0017 (hcp-2) transcript (↓) |
REG0288 | VCA0566 (VxrB) | protein | TCS-RR | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 504728..505384 (+) | 446737785 | Unknown (↑) |
REG0289 | VCA0933 (CspV) | protein | c-di-GMP regulatory pathway | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 886068..886280 (+) | 446027150 | Unknown (↓) |
REG0320 | VC_A0122 | protein | Transcription regulator | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 137379..137621 (+) | 9657509 | hcp-2 expression (↓) |
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|---|---|---|---|---|---|
IMU00156 | VC1419 (TsiV1) | EFF00008 | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002505) | 1516533..1517276 (+) | 15641430 |
IMU00166 | VCA0021 (TsiV2) | EFF00003 | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 26653..27381 (+) | 15600792 |
IMU00172 | VCA0124 (TsiV3) | EFF00005 | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 140631..140999 (+) | 15600895 |
IMU00291 | VC_RS14695 | EFF01442 | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002506) | 305996..306223 (+) | 15601052 |
Experimental investigation has been performed.
Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
---|---|---|---|---|---|---|---|---|
ACP00001 | VC1417 | adaptor | Vibrio cholerae O1 biovar El Tor str. N16961 | chromosome (NC_002505) | 1513748..1514608 (+) | 15641428 | VgrG-1 | VC1418 (TseL) |