Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00002 experimental
T6SS type Type i1
Strain Vibrio cholerae O1 biovar El Tor str. N16961
Replicon (RefSeq) chromosome II (Vas) (NC_002506)
Location 110871..145577
Function -
Comment T6SS introduces cytotoxicity towards Dictyostelium and cultured mammalian macrophages. T6SS is involved in inflammatory response, diarrhoea and actin cross-linking in mouse intestine. T6SS leads to killing of E. coli, Salmonella and Citrobacter rodentium.
References
[1] Dong TG et al (2012) Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52. Nucleic Acids Res. 40(16):7766-75. [PudMed:22723378]
[2] Kitaoka M et al (2011) Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol. 60(Pt 4):397-407. [PudMed:21252269]
[3] Pietrosiuk A et al (2011) Molecular basis for the unique role of the AAA+ chaperone ClpV in type VI protein secretion. J Biol Chem. 286(34):30010-21. [PudMed:21733841]
[4] Altindis E et al (2015) Secretome Analysis of Vibrio cholerae Type VI Secretion System Reveals a New Effector-Immunity Pair. MBio. pii: e00075-15. [PudMed:25759499]
[5] Ishikawa T et al (2012) Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. Infect Immun. 80(2):575-84. [PudMed:22083711]
[6] Zheng J et al (2011) Genetic analysis of anti-amoebae and anti-bacterial activities of the type VI secretion system in Vibrio cholerae. PLoS One. 6(8):e23876. [PudMed:21909372]
[7] Pukatzki S et al (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199]
[8] Borgeaud S et al (2015) Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784]
[9] Dong TG et al (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380]
[10] Basler M et al (2012) Type VI secretion requires a dynamic contractile phage tail-like structure. Nature. 483(7388):182-6. [PudMed:22367545]
[11] Pukatzki S et al (2007) Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc Natl Acad Sci U S A. 104(39):15508-13. [PudMed:17873062]
[12] Miyata ST et al (2011) Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399]
[13] Russell AB et al (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891]
[14] Yang X et al (2014) Molecular mechanism for self-protection against the type VI secretion system in Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 70(Pt 4):1094-103. [PudMed:24699653]
[15] Kitaoka M et al (2011) VasH is a transcriptional regulator of the type VI secretion system functional in endemic and pandemic Vibrio cholerae. J Bacteriol. 193(23):6471-82. [PudMed:21949076]
[16] Miyata ST et al (2013) Dual Expression Profile of Type VI Secretion System Immunity Genes Protects Pandemic Vibrio cholerae. PLoS Pathog. 9(12):e1003752. [PudMed:24348240]
[17] Records AR et al (2011) The type VI secretion system: a multipurpose delivery system with a phage-like machinery. Mol Plant Microbe Interact. 24(7):751-7. [PudMed:21361789]
[18] Ma AT et al (2009) Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133]
[19] Shao Y et al (2014) Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180]
[20] Unterweger D et al (2012) Constitutive type VI secretion system expression gives Vibrio cholerae intra- and interspecific competitive advantages. PLoS One. 7(10):e48320. [PudMed:23110230]
[21] MacIntyre DL et al (2010) The Vibrio cholerae type VI secretion system displays antimicrobial properties. Proc Natl Acad Sci U S A. 107(45):19520-4. [PudMed:20974937]
[22] Broms JE et al (2013) A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552. BMC Microbiol. 13(1):96. [PudMed:23642157]
[23] Jeong JH et al (2014) Purification, crystallization and preliminary X-ray crystallographic analysis of TssL from Vibrio cholerae. Acta Crystallogr F Struct Biol Commun. 70(Pt 9):1260-3. [PudMed:25195905]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPAARTssATssBTssCTssETssFTssGTssHTssITssJTssKTssLTssMVasH
Number112111111111111


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Component ID
Component
Locus tag (Gene)
Coordinate
Strand
Size (bp)
NCBI ID
Product
VC_RS13840106851..107891+1041WP_001134026.1oxidoreductase
VC_RS13845107928..108284-357WP_001249602.1rhodanese-like domain-containing protein
VC_RS13850108299..109855-1557WP_001882958.1EAL domain-containing protein
VC_RS13855110031..110681-651WP_001010777.1HAD family phosphatase
VC_RS13860110871..112649+1779WP_001006513.1SulP family inorganic anion transporter
VC_RS13865112691..114370-1680WP_001275247.1RluA family pseudouridine synthase
T6CP011389PAARVC_RS13870115142..115426+285WP_000526403.1type VI secretion system PAAR protein
VC_RS13875115494..116414+921WP_001901866.1sel1 repeat family protein
T6CP000001TssBVC_RS13880 (tssB)116846..117352+507WP_000031391.1type VI secretion system contractile sheath small subunit
T6CP000002TssCVC_RS13885 (tssC)117396..118871+1476WP_000108140.1type VI secretion system contractile sheath large subunit
Showing 1 to 10 of 36 rows rows per page
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Regulator ID
Locus tag (Gene)
Regulator type
Regulatory pathway
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Target (Regulation)
REG0039 experimentalVC_RS13930 (VasH)proteinGlobal regulatorVibrio cholerae O1 biovar El Tor str. N16961chromosome (NC_002506)128790..130382 (+)446006938VCA0017 (hcp-2) transcript (↓)
REG0288 experimentalVCA0566 (VxrB)proteinTCS-RRVibrio cholerae O1 biovar El Tor str. N16961chromosome (NC_002506)504728..505384 (+)446737785Unknown (↑)
REG0289 experimentalVCA0933 (CspV)proteinc-di-GMP regulatory pathwayVibrio cholerae O1 biovar El Tor str. N16961chromosome (NC_002506)886068..886280 (+)446027150Unknown (↓)
REG0320 experimentalVC_A0122proteinTranscription regulatorVibrio cholerae O1 biovar El Tor str. N16961chromosome (NC_002506)137379..137621 (+)9657509hcp-2 expression (↓)
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experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Showing 1 to 4 of 4 rows

experimental Experimental investigation has been performed.
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Accessory protein ID
Locus tag (Gene)
Type            
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Related structure protein
Related effector
ACP00001 experimentalVC1417adaptorVibrio cholerae O1 biovar El Tor str. N16961chromosome (NC_002505)1513748..1514608 (+)15641428VgrG-1VC1418 (TseL)
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