Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00002 experimental
T6SS type Type i1
Strain Vibrio cholerae O1 biovar El Tor str. N16961
Replicon (RefSeq) chromosome II (Vas) (NC_002506)
Location 110871..145577
Function -
Comment T6SS introduces cytotoxicity towards Dictyostelium and cultured mammalian macrophages. T6SS is involved in inflammatory response, diarrhoea and actin cross-linking in mouse intestine. T6SS leads to killing of E. coli, Salmonella and Citrobacter rodentium.
References
[1] Unterweger D et al (2012) Constitutive type VI secretion system expression gives Vibrio cholerae intra- and interspecific competitive advantages. PLoS One. 7(10):e48320. [PudMed:23110230]
[2] Basler M et al (2012) Type VI secretion requires a dynamic contractile phage tail-like structure. Nature. 483(7388):182-6. [PudMed:22367545]
[3] Ishikawa T et al (2012) Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. Infect Immun. 80(2):575-84. [PudMed:22083711]
[4] Kitaoka M et al (2011) VasH is a transcriptional regulator of the type VI secretion system functional in endemic and pandemic Vibrio cholerae. J Bacteriol. 193(23):6471-82. [PudMed:21949076]
[5] Zheng J et al (2011) Genetic analysis of anti-amoebae and anti-bacterial activities of the type VI secretion system in Vibrio cholerae. PLoS One. 6(8):e23876. [PudMed:21909372]
[6] Pietrosiuk A et al (2011) Molecular basis for the unique role of the AAA+ chaperone ClpV in type VI protein secretion. J Biol Chem. 286(34):30010-21. [PudMed:21733841]
[7] Miyata ST et al (2011) Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399]
[8] Records AR et al (2011) The type VI secretion system: a multipurpose delivery system with a phage-like machinery. Mol Plant Microbe Interact. 24(7):751-7. [PudMed:21361789]
[9] Kitaoka M et al (2011) Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol. 60(Pt 4):397-407. [PudMed:21252269]
[10] MacIntyre DL et al (2010) The Vibrio cholerae type VI secretion system displays antimicrobial properties. Proc Natl Acad Sci U S A. 107(45):19520-4. [PudMed:20974937]
[11] Pukatzki S et al (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199]
[12] Pukatzki S et al (2007) Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc Natl Acad Sci U S A. 104(39):15508-13. [PudMed:17873062]
[13] Ma AT et al (2009) Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133]
[14] Dong TG et al (2012) Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52. Nucleic Acids Res. 40(16):7766-75. [PudMed:22723378]
[15] Dong TG et al (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380]
[16] Russell AB et al (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891]
[17] Broms JE et al (2013) A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552. BMC Microbiol. 13(1):96. [PudMed:23642157]
[18] Miyata ST et al (2013) Dual Expression Profile of Type VI Secretion System Immunity Genes Protects Pandemic Vibrio cholerae. PLoS Pathog. 9(12):e1003752. [PudMed:24348240]
[19] Shao Y et al (2014) Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180]
[20] Yang X et al (2014) Molecular mechanism for self-protection against the type VI secretion system in Vibrio cholerae. Acta Crystallogr D Biol Crystallogr. 70(Pt 4):1094-103. [PudMed:24699653]
[21] Jeong JH et al (2014) Purification, crystallization and preliminary X-ray crystallographic analysis of TssL from Vibrio cholerae. Acta Crystallogr F Struct Biol Commun. 70(Pt 9):1260-3. [PudMed:25195905]
[22] Borgeaud S et al (2015) Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784]
[23] Altindis E et al (2015) Secretome Analysis of Vibrio cholerae Type VI Secretion System Reveals a New Effector-Immunity Pair. MBio. pii: e00075-15. [PudMed:25759499]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHAPAARTssATssBTssCTssETssFTssGTssHTssITssJTssKTssLTssMVasH
Number112111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
VC_RS13840 106851..107891 + 1041 WP_001134026.1 oxidoreductase
VC_RS13845 107928..108284 - 357 WP_001249602.1 rhodanese-like domain-containing protein
VC_RS13850 108299..109855 - 1557 WP_001882958.1 EAL domain-containing protein
VC_RS13855 110031..110681 - 651 WP_001010777.1 HAD family phosphatase
VC_RS13860 110871..112649 + 1779 WP_001006513.1 SulP family inorganic anion transporter
VC_RS13865 112691..114370 - 1680 WP_001275247.1 RluA family pseudouridine synthase
T6CP011389 PAAR VC_RS13870 115142..115426 + 285 WP_000526403.1 type VI secretion system PAAR protein
VC_RS13875 115494..116414 + 921 WP_001901866.1 sel1 repeat family protein
T6CP000001 TssB VC_RS13880 (tssB) 116846..117352 + 507 WP_000031391.1 type VI secretion system contractile sheath small subunit
T6CP000002 TssC VC_RS13885 (tssC) 117396..118871 + 1476 WP_000108140.1 type VI secretion system contractile sheath large subunit
T6CP008374 TssE VC_RS13890 (tssE) 118874..119311 + 438 WP_000221000.1 type VI secretion system baseplate subunit TssE
T6CP000003 TssF VC_RS13895 (tssF) 119317..121086 + 1770 WP_000189792.1 type VI secretion system baseplate subunit TssF
T6CP000004 TssG VC_RS13900 (tssG) 121050..122066 + 1017 WP_000510181.1 type VI secretion system baseplate subunit TssG
T6CP011388 FHA VC_RS13905 (tagH) 122069..123556 + 1488 WP_001089704.1 type VI secretion system-associated FHA domain protein TagH
T6CP000005 TssJ VC_RS13910 (tssJ) 123559..124035 + 477 WP_001045311.1 type VI secretion system lipoprotein TssJ
T6CP000006 TssK VC_RS13915 (tssK) 124042..125376 + 1335 WP_000458007.1 type VI secretion system baseplate subunit TssK
T6CP000007 TssL VC_RS13920 (icmH) 125379..126152 + 774 WP_000083409.1 type IVB secretion system protein IcmH/DotU
T6CP000008 TssH VC_RS13925 (tssH) 126178..128787 + 2610 WP_000619136.1 type VI secretion system ATPase TssH
T6CP012413 VasH VC_RS13930 (vasH) 128790..130382 + 1593 WP_000084793.1 sigma-54 dependent T6SS transcriptional regulator VasH
VC_RS13935 (tagO) 130379..131035 + 657 WP_001223388.1 type VI secretion system-associated protein TagO
T6CP008375 TssA VC_RS13940 (vasJ) 131045..132454 + 1410 WP_000426093.1 TssA family type VI secretion system protein VasJ
T6CP000009 TssM VC_RS13945 (tssM) 132515..136015 + 3501 WP_000225015.1 type VI secretion system membrane subunit TssM
T6CP008376 TssA VC_RS13950 136066..137328 + 1263 WP_000073077.1 ImpA family type VI secretion system protein
T6CP000010 TssI VC_RS13955 (tssI) 137581..140634 + 3054 WP_000113295.1 type VI secretion system tip protein VgrG
VC_RS13960 140631..140999 + 369 WP_001061295.1 hypothetical protein
VC_RS13965 141147..141407 - 261 WP_000799238.1 DUF3012 domain-containing protein
VC_RS13970 (rbsD) 141691..142110 + 420 WP_001911632.1 D-ribose pyranase
VC_RS13975 (rbsA) 142137..143639 + 1503 WP_000189455.1 ribose ABC transporter ATP-binding protein RbsA
VC_RS13980 (rbsC) 143669..144634 + 966 WP_208854928.1 ribose ABC transporter permease
VC_RS13985 (rbsB) 144699..145577 + 879 WP_000737186.1 ribose ABC transporter substrate-binding protein RbsB
VC_RS13990 (rbsK) 145716..146636 + 921 WP_001038380.1 ribokinase
VC_RS13995 146647..147651 + 1005 WP_000224436.1 substrate-binding domain-containing protein
VC_RS14000 147777..148343 + 567 WP_000365205.1 nicotinamide riboside transporter PnuC
VC_RS14005 148389..148511 + 123 WP_000271123.1 hypothetical protein
VC_RS14010 148508..149239 + 732 WP_000027380.1 nucleoside phosphorylase
VC_RS14015 149379..150059 + 681 WP_001177523.1 Crp/Fnr family transcriptional regulator
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0039 experimental VC_RS13930 (VasH) protein Global regulator Vibrio cholerae O1 biovar El Tor str. N16961 chromosome (NC_002506) 128790..130382 (+) 446006938 VCA0017 (hcp-2) transcript (↓)
REG0288 experimental VCA0566 (VxrB) protein TCS-RR Vibrio cholerae O1 biovar El Tor str. N16961 chromosome (NC_002506) 504728..505384 (+) 446737785 Unknown (↑)
REG0289 experimental VCA0933 (CspV) protein c-di-GMP regulatory pathway Vibrio cholerae O1 biovar El Tor str. N16961 chromosome (NC_002506) 886068..886280 (+) 446027150 Unknown (↓)
REG0320 experimental VC_A0122 protein Transcription regulator Vibrio cholerae O1 biovar El Tor str. N16961 chromosome (NC_002506) 137379..137621 (+) 9657509 hcp-2 expression (↓)

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00001 experimental VC1417 adaptor Vibrio cholerae O1 biovar El Tor str. N16961 chromosome (NC_002505) 1513748..1514608 (+) 15641428 VgrG-1 VC1418 (TseL)