SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00007
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00002 (Type i1)
Strain	Vibrio cholerae O1 biovar El Tor str. N16961
Replicon	chromosome
Sequence	Protein sequence (1164 a.a.); Nucleotide sequence (3492 bp)
Description	type VI secretion system Vgr family protein; evolved VgrG with C-terminal actin crosslinking domain (ACD)
Reference	[1] Miyata ST, Kitaoka M, Brooks TM, et al. Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 2011 Jul;79(7):2941-9. doi: 10.1128/IAI.01266-10. Epub 2011 May 9. PMID: 21555399 [2] Pukatzki S, Ma AT, Revel AT, et al. Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15508-13. Epub 2007 Sep 14. PMID: 17873062

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VC_RS06850
Coordinates (Strand)	1510278..1513769 (+)
NCBI ID	15641427
RefSeq	NC_002505
Uniprot ID	VGRG1_VIBCH
KEGG ID	vch:VC1416
PDB ID	4GQK

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ACD	PF16671.7	1.6e-238	726..1111
Phage_GPD	PF05954.13	3.9e-77	41..334
Phage_base_V	PF04717.14	1.1e-13	392..458

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1163	MATLAYSIEVEGLEDETLVVRGFHGQESLSNSVFLGQACYGFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVHGIVRAFSQGDIGHHHTFYQLTLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFALKRDGVQREFCVQYRESDIDFLHRLAAEEGLVYSFVHEAGKHTLYFSDASDSLSKLPEPIPYNALVGGAIDTPYIHGLTYRTQAEVSEVQLKDYSFKKPAYSFLQTVQGTELDYQQTRYQHFDAPGRYKDDVNGAAFSQIRLDYLRRHAHTATGQSNEPLLRAGYKFDLQEHLDPAMNRDWVVVSINHQGEQPQALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGRVKIHFPWDRYSNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLNGDPDQPIITGRTYHATNTPPYTLPEHKTKTVLRTETHQGEGFNELSFEDQAGKEQIYLHAQKDFDGLIENDHTTVIRHDHHLTVENDQFTQIKHNQHLTVEWESREAVTGEQVLSIEGSLHVKTGKVWVNEAGTEIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVNLNSGGSAGSGSGFGGAMPALPGGLEPAVALAPPQTISYQALLQAEQANVPAVKVCPLAAQEATPAVNSITPPPPPPIAPPMAPPQPIMNPQPTANAQPNLGRSTKATPDFPTHFPKSSIGIENELAGLVVAMPANSAQKFGYVKSAQGDALFMLTKDMNQGSYQRPPSLQDGKNYQNWQTHTVELVSYPCEMDDKAAVETRKQAMLWLATHFTTHIDQSNHQPLAPIQSEDGRFVIEITNAKHVIAAGNGISAESQGQTITMTPSGQQATVGVAAKGFGTSATPELRLLESAPWYQKSLKSQFASLTSAENLDDKELAANVFAYLTSIYLKTAELAKKFGIYINEWDPMSEQITPNANGLTDPKVKNAWEILPRTKPSKIVEILSKSDAKAVMKHIKPQLQSRYSESLSKNVFQYFQDGGEVAGHGINNATVGDKHSPELAILFEFRTVPNELQSYLPKTESTTKSEVKLLDQFDPMKRKTVIQQVESLVQNSGDAFDKWYQSYRDSMNQPPVKNAKKIASANQKAQWVKEHNPQEWQRIIA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9832	-	-

Protein sequence: 1164 a.a. .

                                    >EFF00007 NC_002505:1510278-1513769  (Unknown) [Vibrio cholerae O1 biovar El Tor str. N16961]
MATLAYSIEVEGLEDETLVVRGFHGQESLSNSVFLGQACYGFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVH
GIVRAFSQGDIGHHHTFYQLTLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFALKRDGVQREFCVQYRESD
IDFLHRLAAEEGLVYSFVHEAGKHTLYFSDASDSLSKLPEPIPYNALVGGAIDTPYIHGLTYRTQAEVSEVQLKDYSFKK
PAYSFLQTVQGTELDYQQTRYQHFDAPGRYKDDVNGAAFSQIRLDYLRRHAHTATGQSNEPLLRAGYKFDLQEHLDPAMN
RDWVVVSINHQGEQPQALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGRVKIHF
PWDRYSNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLNGDPDQPIITGRTYHATNTPPYTLPEHKTKTVLRTE
THQGEGFNELSFEDQAGKEQIYLHAQKDFDGLIENDHTTVIRHDHHLTVENDQFTQIKHNQHLTVEWESREAVTGEQVLS
IEGSLHVKTGKVWVNEAGTEIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVNLNSGGSAGSGSGFGGAMPA
LPGGLEPAVALAPPQTISYQALLQAEQANVPAVKVCPLAAQEATPAVNSITPPPPPPIAPPMAPPQPIMNPQPTANAQPN
LGRSTKATPDFPTHFPKSSIGIENELAGLVVAMPANSAQKFGYVKSAQGDALFMLTKDMNQGSYQRPPSLQDGKNYQNWQ
THTVELVSYPCEMDDKAAVETRKQAMLWLATHFTTHIDQSNHQPLAPIQSEDGRFVIEITNAKHVIAAGNGISAESQGQT
ITMTPSGQQATVGVAAKGFGTSATPELRLLESAPWYQKSLKSQFASLTSAENLDDKELAANVFAYLTSIYLKTAELAKKF
GIYINEWDPMSEQITPNANGLTDPKVKNAWEILPRTKPSKIVEILSKSDAKAVMKHIKPQLQSRYSESLSKNVFQYFQDG
GEVAGHGINNATVGDKHSPELAILFEFRTVPNELQSYLPKTESTTKSEVKLLDQFDPMKRKTVIQQVESLVQNSGDAFDK
WYQSYRDSMNQPPVKNAKKIASANQKAQWVKEHNPQEWQRIIA*

                                

Nucleotide sequence: 3492 bp .

                                    >EFF00007 NC_002505:1510278-1513769  (Unknown) [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGCGACATTAGCGTACAGCATTGAGGTGGAAGGGCTGGAAGATGAGACTCTGGTGGTCAGAGGCTTCCACGGGCAGGA
GTCACTCTCTAATAGTGTTTTTTTAGGGCAAGCCTGTTACGGGTTTCGCTACGAAGTGCAACTGGCGAGTCGGGTATCAA
ACCTCACCGCCGAGCAGATGGTGGATAAGCGTGCCGAGCTCAAGCTTTACCGCAACTCACAGTTGGTGCAGCGCGTGCAC
GGCATTGTGCGTGCTTTCAGCCAAGGCGATATCGGCCATCACCACACCTTCTACCAACTCACTTTAGTCCCTGCCTTAGA
GCGCTTATCCCTGCGTCATAACAGCCGCATTTTCCAAAAGCAGACTGTCCCTGAGATTCTCTCTATCTTGCTGCAAGAGA
TGGGCATCAACGATTACGCTTTTGCGCTCAAACGTGATGGCGTGCAGCGTGAGTTTTGTGTGCAGTACCGCGAAAGCGAT
ATCGACTTTCTGCACCGCCTCGCCGCTGAAGAAGGTTTGGTGTACAGCTTTGTCCATGAAGCGGGCAAACATACCCTCTA
TTTCAGTGATGCCAGTGATAGCCTAAGCAAACTGCCGGAGCCTATCCCATACAACGCTTTAGTCGGTGGCGCGATAGATA
CCCCGTATATCCACGGCTTAACCTATCGCACCCAAGCAGAAGTGAGCGAAGTTCAGCTCAAAGACTACAGCTTCAAAAAG
CCAGCCTACTCCTTTTTGCAAACCGTGCAGGGTACTGAGCTGGATTATCAGCAAACGCGCTACCAACATTTTGATGCGCC
GGGGCGCTACAAAGACGATGTGAACGGCGCCGCCTTTAGCCAAATCCGTTTAGACTACCTGCGCCGCCATGCCCATACCG
CAACAGGGCAGAGCAACGAGCCGCTGCTGCGTGCCGGCTATAAGTTTGACCTGCAAGAGCACCTTGACCCCGCGATGAAC
CGCGATTGGGTGGTAGTGTCGATTAACCACCAAGGTGAGCAGCCGCAAGCGCTACAAGAAGACGGTGGCAGCGGCGCTAC
TACTTACAGCAATCAGTTCAGCTTAATTCCGGGGCATCTTCACTGGCGCGCCGAGCCACAACCCAAACCGCAAGTCGATG
GTCCAATGATTGCCACGGTCGTCGGTCCTGAAGGCGAAGAAATCTTCTGTGATGAACATGGGCGGGTGAAAATCCATTTC
CCGTGGGACCGTTACTCGAACGGTAACGAGCAAAGCTCCTGTTGGGTACGGGTGTCGCAAGGTTGGGCGGGCAGTCAGTA
TGGCTTTATCGCTATTCCGCGCATCGGCCATGAAGTGATTGTAGAGTTTTTAAACGGTGACCCAGACCAACCGATTATCA
CTGGCCGCACTTATCATGCGACCAACACGCCGCCATACACCTTGCCTGAGCACAAAACCAAAACCGTGCTGCGCACCGAG
ACGCACCAAGGGGAGGGCTTTAACGAGCTCAGTTTTGAAGACCAAGCGGGCAAAGAGCAAATCTACCTGCATGCGCAGAA
AGATTTTGATGGTTTGATTGAAAACGACCACACCACGGTCATTCGTCACGACCATCACCTGACCGTTGAAAACGACCAAT
TTACCCAGATTAAGCACAACCAACACCTGACGGTGGAGTGGGAAAGTCGAGAAGCGGTAACGGGCGAGCAAGTGCTGAGT
ATTGAAGGCTCGCTGCATGTGAAAACAGGCAAAGTTTGGGTCAATGAAGCGGGTACAGAGATCCATGTAAAAGCGGGACA
GAAAGTGGTGATTGAAGCGGGCTCCGAGATTACCGTTAAAGCGGGCGGCAGCTTTGTGAAAGTCGATCCGGCTGGGGTGC
ATCTGTCCGGTGCTTTGGTCAATCTCAACTCTGGCGGCAGCGCTGGTAGTGGTAGCGGCTTTGGCGGTGCAATGCCCGCC
TTACCGGGCGGCTTAGAACCCGCCGTGGCACTGGCTCCACCGCAAACCATCTCTTACCAAGCGTTGCTACAGGCCGAGCA
AGCGAATGTTCCTGCGGTGAAGGTGTGTCCTTTAGCCGCTCAAGAAGCAACGCCTGCGGTGAATAGTATCACGCCGCCTC
CACCACCACCGATTGCTCCGCCAATGGCCCCACCACAACCAATAATGAATCCACAGCCAACCGCTAACGCGCAGCCTAAT
CTTGGAAGATCCACTAAGGCAACGCCGGATTTTCCAACACATTTCCCGAAGTCATCCATTGGCATTGAGAATGAGTTAGC
TGGTCTAGTGGTGGCTATGCCAGCCAACTCGGCACAGAAATTTGGCTACGTGAAAAGTGCACAAGGTGATGCGCTGTTTA
TGTTAACGAAAGACATGAACCAAGGTTCGTATCAGCGGCCACCTTCATTACAAGATGGAAAAAATTATCAGAATTGGCAG
ACGCATACAGTAGAGTTGGTGAGCTACCCTTGTGAAATGGACGATAAAGCTGCCGTTGAAACACGCAAACAAGCCATGTT
GTGGTTAGCAACCCACTTTACTACGCATATCGACCAGTCCAATCATCAACCATTAGCTCCTATTCAAAGTGAAGACGGAC
GCTTTGTTATCGAAATCACTAATGCGAAGCATGTGATTGCTGCGGGCAATGGGATTTCTGCAGAATCTCAAGGTCAAACA
ATCACGATGACACCAAGTGGACAACAAGCCACAGTGGGTGTTGCTGCTAAAGGTTTCGGGACGAGTGCAACGCCAGAATT
AAGGTTACTTGAATCGGCACCTTGGTATCAGAAATCACTCAAGAGCCAATTTGCATCTCTAACGAGCGCAGAAAACTTGG
ATGATAAAGAGTTAGCTGCAAACGTATTTGCTTATCTGACGTCTATTTATTTAAAAACAGCAGAGCTAGCGAAGAAGTTT
GGTATTTACATCAATGAATGGGACCCCATGTCGGAGCAAATTACTCCAAATGCGAATGGATTAACGGATCCAAAAGTAAA
AAATGCATGGGAAATATTGCCACGTACAAAACCAAGCAAAATAGTAGAAATACTCAGTAAGAGTGATGCCAAAGCGGTTA
TGAAACATATCAAGCCTCAATTGCAGTCTCGCTATTCTGAGAGTTTGTCGAAAAATGTATTTCAATATTTCCAAGATGGT
GGTGAAGTAGCCGGACACGGTATTAATAATGCGACGGTCGGTGATAAACATAGTCCGGAATTGGCGATTTTGTTTGAGTT
CAGAACTGTTCCGAATGAACTGCAAAGTTATTTGCCTAAAACAGAATCGACAACTAAGTCAGAAGTGAAGTTACTCGATC
AGTTTGATCCTATGAAGCGTAAAACAGTAATTCAACAAGTAGAATCGCTTGTGCAAAATAGTGGCGATGCATTTGATAAA
TGGTACCAAAGCTACCGAGACTCCATGAATCAACCTCCCGTTAAAAATGCGAAGAAAATTGCTTCCGCGAATCAAAAAGC
ACAATGGGTTAAAGAGCATAATCCTCAAGAATGGCAACGCATTATTGCTTAG

                                