SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00008
Effector type	Tle
Cognate immunity protein	IMU00156
Related T6SS (Type)	T6SS00002 (Type i1)
Strain	Vibrio cholerae O1 biovar El Tor str. N16961
Replicon	chromosome
Sequence	Protein sequence (642 a.a.); Nucleotide sequence (1926 bp)
Description	TseL (Tle2); lipase
Reference	[1] Dong TG, Ho BT, Yoder-Himes DR, et al. Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A. 2013 Feb 12;110(7):2623-8. doi: 10.1073/pnas.1222783110. Epub 2013 Jan 29. PMID: 23362380 [2] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891 [3] Liang X, Moore R, Wilton M, et al. Identification of divergent type VI secretion effectors using a conserved chaperone domain. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9106-11. doi: 10.1073/pnas.1505317112. Epub 2015 Jul 6. PMID: 26150500

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VC1418 (TseL, Tle2)
Coordinates (Strand)	1514611..1516536 (+)
NCBI ID	15641429
RefSeq	NC_002505
Uniprot ID	TSEL_VIBCH
KEGG ID	vch:VC1418
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Lipase_3	PF01764.27	5e-18	320..433

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-641	MDSFNYCVQCNPEENWLELEFRSENDEPIDGLLVTITNQSAPSNTYTQTTSSGKVLFGKIAAGEWRASVSQASLLTEVEKYASRKEGQESPVKKRAAAELDAADKDTKQYRFTTIGDFWDEAPKDEFLQKQHKGIDVNASAEKAGFRLSHNQTYVFEIKALRSYMPVIIDTDEFNLVNSYTFALLSKLAYATNDFNRDDGKTIDNQGAISTVISQLKRKERPTYSGDLQAKWLLEEIPYSKALSAQYYAEDDVGSEGYIIFNDELAIIGVRGTEPYFQSKKPPVDNTKFKIIKAASGMAAVIADKIESATDSPGMKDLIITDLDAAQIAPEEFGGTYVHRGFYQYTMALLSLMEKDLGLHKIKKFYCCGHSLGGAGALLISALIKDSYHPPVLRLYTYGMPRVGTRSFVERYQNILHYRHVNNHDLVPQIPTVWMNTDVSEGFHVLDVFKSRVDLMRKMLTDDDDDNYQHHGHLSQLLTYNSNNQVLLTPKQTQVTMLDLANLATNDSVAMVDGLSDASIVEHGMEQYIPNLFEQLTALSDESLMVHYQRAISALEQEIATLQQSYLTVKQAWIESIGNGTPTMNIGRLMSEMHSINKLIENRNKIRGELRQIVSDPQRMPATKFLISQQTLPDEIKVQIR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9852	-	-

Protein sequence: 642 a.a. .

                                    >EFF00008 NC_002505:1514611-1516536 Type VI Lipase Effector (Tle) [Vibrio cholerae O1 biovar El Tor str. N16961]
MDSFNYCVQCNPEENWLELEFRSENDEPIDGLLVTITNQSAPSNTYTQTTSSGKVLFGKIAAGEWRASVSQASLLTEVEK
YASRKEGQESPVKKRAAAELDAADKDTKQYRFTTIGDFWDEAPKDEFLQKQHKGIDVNASAEKAGFRLSHNQTYVFEIKA
LRSYMPVIIDTDEFNLVNSYTFALLSKLAYATNDFNRDDGKTIDNQGAISTVISQLKRKERPTYSGDLQAKWLLEEIPYS
KALSAQYYAEDDVGSEGYIIFNDELAIIGVRGTEPYFQSKKPPVDNTKFKIIKAASGMAAVIADKIESATDSPGMKDLII
TDLDAAQIAPEEFGGTYVHRGFYQYTMALLSLMEKDLGLHKIKKFYCCGHSLGGAGALLISALIKDSYHPPVLRLYTYGM
PRVGTRSFVERYQNILHYRHVNNHDLVPQIPTVWMNTDVSEGFHVLDVFKSRVDLMRKMLTDDDDDNYQHHGHLSQLLTY
NSNNQVLLTPKQTQVTMLDLANLATNDSVAMVDGLSDASIVEHGMEQYIPNLFEQLTALSDESLMVHYQRAISALEQEIA
TLQQSYLTVKQAWIESIGNGTPTMNIGRLMSEMHSINKLIENRNKIRGELRQIVSDPQRMPATKFLISQQTLPDEIKVQI
R*

                                

Nucleotide sequence: 1926 bp .

                                    >EFF00008 NC_002505:1514611-1516536 Type VI Lipase Effector (Tle) [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGATTCATTTAATTATTGCGTGCAGTGTAACCCTGAGGAAAACTGGTTAGAGCTAGAGTTTCGGAGTGAAAATGATGA
ACCGATAGATGGTTTGCTAGTTACGATTACGAACCAAAGTGCCCCCTCTAACACATACACGCAAACCACAAGTTCGGGTA
AGGTGTTGTTTGGCAAGATTGCGGCTGGGGAATGGCGTGCTTCTGTAAGCCAAGCTTCTCTGTTAACTGAAGTCGAAAAA
TATGCCAGCCGTAAAGAGGGACAAGAGTCGCCAGTGAAAAAACGAGCTGCGGCAGAACTTGACGCCGCAGATAAAGATAC
GAAGCAGTACCGCTTTACCACTATTGGTGATTTCTGGGATGAAGCACCAAAAGATGAGTTTTTACAAAAGCAGCATAAAG
GGATAGATGTCAATGCTTCTGCTGAGAAAGCAGGGTTTAGACTCAGTCATAACCAGACCTATGTTTTTGAAATCAAAGCA
CTACGTAGCTATATGCCAGTGATTATTGATACCGATGAATTTAACTTGGTGAATAGTTATACCTTTGCGTTGTTGTCGAA
ATTAGCTTATGCGACTAACGATTTTAATAGGGATGATGGTAAGACAATAGATAATCAGGGAGCAATTAGCACGGTTATTT
CTCAATTGAAGCGTAAAGAAAGACCGACTTATTCTGGAGATTTACAAGCAAAGTGGTTATTAGAGGAAATTCCATATAGC
AAAGCGTTAAGTGCCCAATATTATGCTGAGGATGATGTCGGTAGTGAAGGTTACATCATCTTTAACGATGAATTGGCGAT
TATAGGTGTGAGAGGTACAGAGCCTTATTTTCAGAGTAAGAAACCACCTGTTGATAACACTAAATTTAAAATTATAAAAG
CGGCTTCAGGTATGGCGGCTGTGATTGCGGATAAGATAGAAAGTGCTACCGATTCACCAGGAATGAAAGACCTTATTATA
ACAGACCTTGATGCTGCGCAAATCGCCCCCGAAGAGTTTGGTGGAACATATGTTCATCGGGGGTTTTACCAATATACGAT
GGCTTTGTTATCTTTGATGGAGAAAGATCTAGGGCTACATAAAATAAAAAAATTCTATTGTTGTGGCCATAGCTTAGGGG
GCGCTGGTGCATTACTTATAAGTGCATTGATTAAAGATAGCTATCATCCTCCAGTATTGCGTTTATATACATATGGAATG
CCACGAGTGGGAACTCGCAGTTTTGTAGAGCGCTATCAAAATATTTTGCATTACCGTCATGTTAATAATCACGATTTGGT
GCCACAGATACCAACAGTATGGATGAACACAGATGTATCTGAAGGATTCCATGTACTTGATGTATTTAAAAGCCGTGTTG
ATCTGATGAGAAAAATGCTCACTGATGATGATGATGACAATTATCAACACCATGGTCATTTGTCTCAGTTACTAACTTAT
AATTCAAATAACCAAGTCTTACTGACACCCAAACAAACTCAAGTCACTATGCTAGACCTAGCAAATTTAGCCACTAATGA
TTCTGTTGCTATGGTAGATGGTTTATCAGATGCCTCAATAGTAGAGCATGGAATGGAGCAGTATATACCCAATTTATTTG
AGCAGTTAACAGCACTAAGTGATGAAAGTTTAATGGTGCATTATCAACGAGCGATTTCAGCCTTAGAGCAGGAAATTGCT
ACGTTGCAACAAAGTTATCTTACAGTTAAACAAGCTTGGATTGAATCTATAGGAAATGGCACGCCGACTATGAATATCGG
ACGTTTGATGAGTGAAATGCATTCTATAAATAAATTAATAGAGAACCGTAATAAAATTAGAGGTGAGCTAAGGCAGATAG
TCTCTGATCCTCAGCGTATGCCTGCAACTAAATTTTTAATTTCACAGCAAACCTTACCAGATGAAATCAAGGTGCAAATA
AGATGA

                                