SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00005
Effector type	Tge
Cognate immunity protein	IMU00172
Related T6SS (Type)	T6SS00002 (Type i1)
Strain	Vibrio cholerae O1 biovar El Tor str. N16961
Replicon	chromosome
Sequence	Protein sequence (1018 a.a.); Nucleotide sequence (3054 bp)
Description	type VI secretion system Vgr family protein; Muramidase
Reference	[1] Dong TG, Ho BT, Yoder-Himes DR, et al. Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A. 2013 Feb 12;110(7):2623-8. doi: 10.1073/pnas.1222783110. Epub 2013 Jan 29. PMID: 23362380 [2] Brooks TM, Unterweger D, Bachmann V, et al. Lytic activity of the Vibrio cholerae type VI secretion toxin VgrG-3 is inhibited by the antitoxin TsaB. J Biol Chem. 2013 Mar 15;288(11):7618-7625. doi: 10.1074/jbc.M112.436725. Epub 2013 Jan 22. PMID: 23341465

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VCA0123
Coordinates (Strand)	137581..140634 (+)
NCBI ID	15600894
RefSeq	NC_002506
Uniprot ID	VGRG3_VIBCH
KEGG ID	vch:VCA0123
PDB ID	4NOO, 4NSO

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	4.6e-70	41..334
Phage_base_V	PF04717.14	5e-15	391..458
PG_binding_1	PF01471.20	4.4e-08	731..773
DUF2345	PF10106.11	9.7e-05	570..618

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1017	MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRCEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQINGIVRQLSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETDLQFLHRIAAEEGLVYSHLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKKPAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDANGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDHAMNRDWLVVQANHQGTQPQALQEEGGSGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHFPWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYALPDHKTKTVLRTETHQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFTRIQRNQHLTVEGEVRSKIALDSSHEVGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAAAPRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANDEPVAEEKTPERILKSDLLKPSDELEKLAKRQASAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGAVDGIVGKGTLLALDEALMDGWVYENNIYQIWPLGKTSEKYESAGRGPGVISTGNGDYGGASYGCYQMSSNLGVVQKYIQSSKFKEFFSGLNPATKEFNVVWQDIASRYPQEFREEQHQFIKRTHYDIQIGHLRGKGLLFEHNRAAVHDLIWSTSVQFGGRTNLIFNALNGQNMESMTDKDIIILVQDYKLVNTERLFKSSPSWWSDLKKRAVSEKKALLELEIDGLEVDIK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9951	-	-

Protein sequence: 1018 a.a. .

                                    >EFF00005 NC_002506:137581-140634 Type VI Glycoside hydrolase Effector (Tge) [Vibrio cholerae O1 biovar El Tor str. N16961]
MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRCEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQIN
GIVRQLSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETD
LQFLHRIAAEEGLVYSHLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKK
PAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDANGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDHAMN
RDWLVVQANHQGTQPQALQEEGGSGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHF
PWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYALPDHKTKTVLRTE
THQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFTRIQRNQHLTVEGEVRSKIALDSSHE
VGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAA
APRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANDEPVAEEKTPERILKSDLLKPSDELEKLAKR
QASAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGAVDGIVGKGTLLALD
EALMDGWVYENNIYQIWPLGKTSEKYESAGRGPGVISTGNGDYGGASYGCYQMSSNLGVVQKYIQSSKFKEFFSGLNPAT
KEFNVVWQDIASRYPQEFREEQHQFIKRTHYDIQIGHLRGKGLLFEHNRAAVHDLIWSTSVQFGGRTNLIFNALNGQNME
SMTDKDIIILVQDYKLVNTERLFKSSPSWWSDLKKRAVSEKKALLELEIDGLEVDIK*

                                

Nucleotide sequence: 3054 bp .

                                    >EFF00005 NC_002506:137581-140634 Type VI Glycoside hydrolase Effector (Tge) [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGCAAGGTTACAGTTTCAATTAAAGGTGGATGGCCTTGAGGATGAATCCCTCGTCGTACGCGGATTTGAAGGGCAAGA
GTCCTTGTCTGATTCTGTATGGCGCTGTGAACCCTGCTATGGTTTTCGCTATCAGGTTGATTTAGCCAGTGCGCTAAGCA
ACCTTACTGCTGAGCAATTTGTCGACCAAACCGCGCATTTAACCATTCTGCGTGACGGGCAAGTGGTGCAGCAAATTAAC
GGTATCGTACGCCAATTGAGTAAAGGCGATACTGGGCATCGGCACACGTTTTATTCTCTTACGTTAGTACCTGCGCTGGA
GCGGCTTTCTTTACGCAGTAATAGCCGTATTTTCCAGCAGCAAAGCGTGCCGGAAATTATCTCTATTTTACTGCAAGAGA
TGGGGATTGAAGATTACGCCTTTGCCCTTAAGCGTGAGTGTGCCCAGCGTGAGTTTTGTGTGCAGTACCGTGAAACGGAT
TTGCAGTTTTTACACCGTATCGCCGCCGAAGAAGGCTTGGTATACAGTCACCTACATGAAGCGCAGAAACACACGCTACT
GTTTACCGATAGCTCGGACAGCCAACCGAAGTTAGCCAAGCCAGTACCTTATAATGCGCTGGCGGGTGGCGAGATCAATC
TCCCTTATGTGGTCGATCTGCAATTCAAGACCACCGCACAAGTCAGCCATACCGAGCTAAAGGATTACAGCTTTAAAAAG
CCAGCTTACGGATTTACTCAGCGCACGCAGGGTAAAGATATCGCTTATCAGCAGCCAAATTATGAGCATTTTGATGCTCC
GGGACGCTACAAAGACGATGCAAATGGCAAGGCCTTTAGCCAAATTCGCTTAGAGTATTTACGTCGCGATGCTTTGCTTG
CTGATGCGAAGAGTGACGAACCTTTGCTGTTGGCGGGAGTGCGTTTTGACTTGCAAGATCACCTTGATCACGCAATGAAT
CGAGATTGGCTCGTGGTACAAGCCAATCACCAAGGGACGCAGCCACAAGCGTTACAAGAAGAGGGTGGTTCAGGCGCTAC
CACTTACAGCAATCAGCTAAAACTTATTCCCGCTCACATCACTTGGCGAGCAAGGCCTTGTGCTAAGCCGCAAGTGGATG
GTCCCATGATCGCCACCGTGGTTGGGCCACAAGGTGAAGAGATTTATTGCGATAACTTTGGTCGCGTGAAAGTGCATTTC
CCGTGGGATCGCTACTCAAGTAGCAACGAAAAAAGCTCTTGCTGGGTGCGAGTGGCACAAGAATGGGCTGGTAGCCAATA
CGGTAGTATGGCGATTCCGCGAGTTGGCCATGAAGTGATTGTTTCGTTCCTGAATGGCGATCCGGATCAACCCATCATCA
CAGGTCGTACGTATCATGCGACCAATACCGCGCCTTACGCCTTGCCTGACCACAAAACCAAAACCGTGCTGCGCACTGAG
ACTCACCAAGGGCAAGGCTACAACGAACTGAGTTTTGAGGATCAAGCGGGCAGCGAACAGATTTTGCTGCATGCACAAAA
AGATTGGGATGCGTTGATTGAGCATGATCACACCGAAGTGATTCGCCACGATCAACATCTCACTGTGGACAATGATCGCT
TTACCCGCATTCAGCGCAACCAACATTTAACGGTTGAAGGTGAAGTCCGTAGCAAAATCGCACTCGATAGCAGCCATGAA
GTCGGCGCGTCACTACAACACAAAGTCGGGCAGCGCATTGCTGTCGAGGCGGGTAAAGAGATTTCACTCAAAAGCGGCGC
AAAAATCGTGGTTGAAGCCGGAGCGGAGTTAACCCTAAAAGCTGGGGGCAGTTTTGTAAAAGTCGATGCTGGTGGCGTGC
ACTTAGTCGGTCCTGCCATTAACCTAAACGCGGGCGGCAGTGCAGGCAGCGGTAGCGCTTATGGCGGGCAATTAGCGGCA
GCACCGAGAATGTTAGCGCAAGCTAAACCAGTAGCAGAATTGGTTCAGCCGGATATTGCGGCCTCAATGCAATCAGGCGC
GGCGCGTGTTATTGATGTGGCATCACTCCCCACAATGATGCCGAGTTCAGCCAATAACACGGCAAATGATGAGCCTGTGG
CCGAAGAGAAAACACCGGAGCGAATTTTAAAATCCGATCTCCTCAAACCATCCGATGAGTTAGAGAAACTCGCCAAGCGA
CAAGCGAGTGCTTATCGTCAAGGTAATCACAGCGATGAGGTTAAGTTACTACAAGAAGCGCTCATAAAATTGGGGTTTGA
TCTCGGGAAAGCAGGAGCTGATGGTGACTTTGGGAGCAAAACTAAAACCGCGATTGAGCAGTTCCAGAAAAGCTATCAAC
CAAGCCACCAAACCCACCCGTCTTACAGCATTGGCGCTGTTGACGGTATTGTCGGCAAAGGTACACTACTGGCACTAGAC
GAAGCCTTGATGGATGGGTGGGTGTATGAGAATAATATTTATCAAATATGGCCTCTAGGTAAAACGTCAGAGAAATATGA
GTCGGCAGGAAGAGGCCCAGGGGTTATATCAACAGGAAATGGAGATTATGGTGGGGCTTCATATGGTTGCTATCAAATGT
CATCAAATCTTGGTGTGGTACAAAAATATATTCAGTCATCAAAATTCAAAGAATTCTTTAGTGGATTAAATCCCGCTACG
AAAGAGTTTAATGTTGTTTGGCAGGATATAGCTTCAAGATATCCTCAGGAATTCAGAGAAGAACAACACCAATTTATTAA
GAGAACTCATTATGATATACAAATAGGACATCTTAGAGGGAAAGGACTTTTGTTTGAACATAATCGAGCTGCTGTACATG
ATTTGATCTGGTCTACTTCAGTACAGTTTGGTGGGAGAACTAATTTGATTTTCAATGCATTGAATGGACAAAATATGGAA
AGTATGACTGATAAGGACATCATTATTCTGGTACAAGATTACAAGCTTGTTAATACAGAAAGGCTTTTTAAGTCTTCGCC
ATCATGGTGGAGTGATTTAAAAAAACGTGCGGTATCAGAGAAAAAAGCTTTACTTGAACTAGAAATTGACGGTTTGGAGG
TTGATATAAAATGA

                                