Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00166  |
| Cognate effector | EFF00003 |
| Related T6SS (Type) | T6SS00002 (Type i1) |
| Strain | Vibrio cholerae O1 biovar El Tor str. N16961 |
| Replicon | chromosome |
| Sequence | Protein sequence (243 a.a.); Nucleotide sequence (729 bp) |
| Description | TsiV2 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | VCA0021 (TsiV2) |
| Coordinates (Strand) | 26653..27381 (+) |
| NCBI ID | 15600792 |
| RefSeq | NC_002506 |
| Uniprot ID | TSIV2_VIBCH |
| KEGG ID | vch:VCA0021 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-38 | MLIDKNELEQLKVKLHSSEVIYQWDSVAYGERRSEIFR |
| Transmembrane helix | 39-61 | VFGAISAGIVPLWPFIFFADIQF |
| Outside | 62-65 | NSKE |
| Transmembrane helix | 66-85 | FWGFICFSLAGMAAARYLFM |
| Inside | 86-116 | PDHRYCYSLTQAGIYYTDQEVIPDAAYTFVR |
| Transmembrane helix | 117-139 | GFAWVGIAVCLLALAVVGPLAFV |
| Outside | 140-242 | GAGGFALLAFGLTNFHPTVHKKEVYFADQLIVFDPIKEKMVDLNTDSTDEPWFDRRLFFSSLDEKTHFIELVKSIHNNVDYLPLQRVNDQYKHPIFNQELKEE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9583 | - | - |
Protein sequence: 243 a.a. .
>IMU00166 NC_002506:26653-27381 [Vibrio cholerae O1 biovar El Tor str. N16961]
MLIDKNELEQLKVKLHSSEVIYQWDSVAYGERRSEIFRVFGAISAGIVPLWPFIFFADIQFNSKEFWGFICFSLAGMAAA
RYLFMPDHRYCYSLTQAGIYYTDQEVIPDAAYTFVRGFAWVGIAVCLLALAVVGPLAFVGAGGFALLAFGLTNFHPTVHK
KEVYFADQLIVFDPIKEKMVDLNTDSTDEPWFDRRLFFSSLDEKTHFIELVKSIHNNVDYLPLQRVNDQYKHPIFNQELK
EE*
MLIDKNELEQLKVKLHSSEVIYQWDSVAYGERRSEIFRVFGAISAGIVPLWPFIFFADIQFNSKEFWGFICFSLAGMAAA
RYLFMPDHRYCYSLTQAGIYYTDQEVIPDAAYTFVRGFAWVGIAVCLLALAVVGPLAFVGAGGFALLAFGLTNFHPTVHK
KEVYFADQLIVFDPIKEKMVDLNTDSTDEPWFDRRLFFSSLDEKTHFIELVKSIHNNVDYLPLQRVNDQYKHPIFNQELK
EE*
Nucleotide sequence: 729 bp .
>IMU00166 NC_002506:26653-27381 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGTTAATTGATAAAAATGAGTTAGAGCAGTTAAAGGTAAAATTACACAGCAGTGAGGTTATTTATCAATGGGATAGTGT
GGCTTACGGAGAGAGAAGATCTGAAATATTTCGCGTTTTTGGGGCAATCTCTGCTGGTATTGTTCCCCTTTGGCCATTTA
TTTTTTTTGCAGATATTCAATTTAATAGTAAAGAGTTTTGGGGATTTATCTGCTTTAGTCTGGCGGGCATGGCTGCTGCC
AGATATCTTTTTATGCCCGACCACCGTTATTGCTACAGTTTAACGCAAGCGGGGATTTATTATACGGATCAAGAAGTGAT
CCCAGATGCGGCCTACACTTTTGTGCGTGGTTTTGCTTGGGTGGGGATTGCGGTCTGTTTGTTAGCCTTAGCCGTCGTTG
GCCCGTTAGCGTTTGTCGGTGCGGGCGGTTTTGCCCTGCTGGCCTTTGGTTTGACCAACTTCCACCCCACCGTACATAAG
AAAGAGGTTTACTTTGCTGACCAATTGATTGTTTTTGATCCCATCAAAGAAAAGATGGTGGATTTAAATACAGATAGCAC
AGATGAACCATGGTTTGACAGACGGCTATTTTTTAGTTCACTTGATGAGAAGACGCATTTTATTGAGCTCGTGAAGAGTA
TTCATAATAATGTCGACTATCTTCCGTTACAGCGAGTTAACGATCAGTACAAGCATCCAATCTTTAATCAAGAATTAAAA
GAGGAATAG
ATGTTAATTGATAAAAATGAGTTAGAGCAGTTAAAGGTAAAATTACACAGCAGTGAGGTTATTTATCAATGGGATAGTGT
GGCTTACGGAGAGAGAAGATCTGAAATATTTCGCGTTTTTGGGGCAATCTCTGCTGGTATTGTTCCCCTTTGGCCATTTA
TTTTTTTTGCAGATATTCAATTTAATAGTAAAGAGTTTTGGGGATTTATCTGCTTTAGTCTGGCGGGCATGGCTGCTGCC
AGATATCTTTTTATGCCCGACCACCGTTATTGCTACAGTTTAACGCAAGCGGGGATTTATTATACGGATCAAGAAGTGAT
CCCAGATGCGGCCTACACTTTTGTGCGTGGTTTTGCTTGGGTGGGGATTGCGGTCTGTTTGTTAGCCTTAGCCGTCGTTG
GCCCGTTAGCGTTTGTCGGTGCGGGCGGTTTTGCCCTGCTGGCCTTTGGTTTGACCAACTTCCACCCCACCGTACATAAG
AAAGAGGTTTACTTTGCTGACCAATTGATTGTTTTTGATCCCATCAAAGAAAAGATGGTGGATTTAAATACAGATAGCAC
AGATGAACCATGGTTTGACAGACGGCTATTTTTTAGTTCACTTGATGAGAAGACGCATTTTATTGAGCTCGTGAAGAGTA
TTCATAATAATGTCGACTATCTTCCGTTACAGCGAGTTAACGATCAGTACAAGCATCCAATCTTTAATCAAGAATTAAAA
GAGGAATAG