Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KYK85_RS01955 Genome accession   NZ_CP079992
Coordinates   421060..421737 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain KMS2001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416060..426737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYK85_RS01940 (KYK85_01935) chiA 417418..420120 (+) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -
  KYK85_RS01945 (KYK85_01940) bfd 420289..420483 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KYK85_RS01950 (KYK85_01945) bfr 420555..421031 (+) 477 WP_000675504.1 bacterioferritin -
  KYK85_RS01955 (KYK85_01950) pilD 421060..421737 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  KYK85_RS01960 (KYK85_01955) gspM 421737..422198 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  KYK85_RS01965 (KYK85_01960) gspL 422195..423358 (-) 1164 WP_001332436.1 type II secretion system protein GspL -
  KYK85_RS01970 (KYK85_01965) gspK 423373..424356 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  KYK85_RS01975 (KYK85_01970) gspJ 424349..424936 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  KYK85_RS01980 (KYK85_01975) gspI 424929..425324 (-) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  KYK85_RS01985 (KYK85_01980) gspH 425321..425830 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  KYK85_RS01990 (KYK85_01985) gspG 425838..426275 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=516142 KYK85_RS01955 WP_000178175.1 421060..421737(-) (pilD) [Escherichia coli strain KMS2001]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=516142 KYK85_RS01955 WP_000178175.1 421060..421737(-) (pilD) [Escherichia coli strain KMS2001]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387