Detailed information NTG0398924

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: GH975_RS10250
• Genome accession: NZ_CP045871
• Coordinates: 2015124..2015522 (-)
• Length: 132 a.a.
• NCBI ID: WP_153714431.1
• Uniprot ID: A0A5Q2Q9J1
• Organism: Litorivicinus lipolyticus strain IMCC 1097
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2010124..2020522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GH975_RS10225 (GH975_10225)	-	2010327..2011295 (+)	969	WP_153714426.1	asparaginase	-
GH975_RS10230 (GH975_10230)	-	2011282..2012544 (-)	1263	WP_153714427.1	hypothetical protein	-
GH975_RS10235 (GH975_10235)	-	2012578..2013354 (+)	777	WP_153714428.1	YdcF family protein	-
GH975_RS10240 (GH975_10240)	-	2013439..2014386 (+)	948	WP_153714429.1	diguanylate cyclase domain-containing protein	-
GH975_RS12275 (GH975_10245)	pilA	2014440..2014904 (-)	465	WP_153714430.1	pilin	Machinery gene
GH975_RS10250 (GH975_10250)	pilA2	2015124..2015522 (-)	399	WP_153714431.1	pilin	Machinery gene
GH975_RS10255 (GH975_10255)	pilB	2015727..2017424 (+)	1698	WP_153714432.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GH975_RS10260 (GH975_10260)	pilC	2017437..2018681 (+)	1245	WP_153714433.1	type II secretion system F family protein	Machinery gene
GH975_RS10265 (GH975_10265)	pilD	2018706..2019593 (+)	888	WP_153714434.1	A24 family peptidase	Machinery gene
GH975_RS10270 (GH975_10270)	coaE	2019590..2020189 (+)	600	WP_153714435.1	dephospho-CoA kinase	-
GH975_RS10275 (GH975_10275)	-	2020186..2020395 (+)	210	WP_153714436.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 132 a.a.
• Molecular weight: 13199.13 Da
• Isoelectric Point: 6.1229

>NTDB_id=398924 GH975_RS10250 WP_153714431.1 2015124..2015522(-) (pilA2) [Litorivicinus lipolyticus strain IMCC 1097]
MKNQKGFTLIELMIVVAIVGILAAVAIPAYSDYTTRAKITEGLSLMSGAKTAVTENLLSGNGASSGVNDLGVTGANVASV
TTASATGAITVTYNANVAGGGNIVFTPTFANGVVSWVCTSAINDALLPATCR

Nucleotide

• Length: 399 bp

>NTDB_id=398924 GH975_RS10250 WP_153714431.1 2015124..2015522(-) (pilA2) [Litorivicinus lipolyticus strain IMCC 1097]
ATGAAGAATCAAAAAGGCTTTACGCTAATCGAACTGATGATCGTGGTTGCGATCGTTGGCATCTTGGCCGCTGTCGCGAT
CCCTGCGTACTCGGACTACACCACGCGCGCCAAAATTACCGAAGGCTTAAGCCTGATGTCAGGTGCCAAAACGGCGGTGA
CTGAAAACCTATTAAGCGGCAACGGTGCAAGCTCCGGTGTTAACGATCTGGGCGTGACCGGTGCCAACGTCGCCAGCGTC
ACCACTGCGTCGGCGACCGGCGCGATCACTGTAACCTACAACGCCAACGTCGCCGGCGGCGGTAACATCGTATTCACGCC
GACCTTTGCCAACGGCGTGGTCAGCTGGGTGTGTACTTCTGCTATCAACGATGCTTTGTTGCCGGCGACCTGCCGCTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-132)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

3D structure

• AlphaFold DB: A0A5Q2Q9J1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	55.474	100	0.576
pilA2	Legionella pneumophila strain ERS1305867	54.015	100	0.561
comP	Acinetobacter baylyi ADP1	49.315	100	0.545
pilA	Ralstonia pseudosolanacearum GMI1000	50	100	0.508
pilE	Neisseria gonorrhoeae MS11	40.764	100	0.485
pilE	Neisseria gonorrhoeae strain FA1090	38.65	100	0.477
pilA/pilA1	Eikenella corrodens VA1	39.333	100	0.447
pilA	Pseudomonas aeruginosa PAK	38.667	100	0.439
pilA	Haemophilus influenzae Rd KW20	41.045	100	0.417
pilA	Vibrio parahaemolyticus RIMD 2210633	42.52	96.212	0.409
pilA	Haemophilus influenzae 86-028NP	39.552	100	0.402
pilA	Acinetobacter baumannii strain A118	37.41	100	0.394
pilA	Glaesserella parasuis strain SC1401	37.778	100	0.386
pilA/pilAI	Pseudomonas stutzeri DSM 10701	37.5	100	0.386
pilA/pilAII	Pseudomonas stutzeri DSM 10701	37.313	100	0.379
pilA	Vibrio cholerae C6706	33.566	100	0.364
pilA	Vibrio cholerae strain A1552	33.566	100	0.364
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	33.566	100	0.364