Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GH975_RS12275 Genome accession   NZ_CP045871
Coordinates   2014440..2014904 (-) Length   154 a.a.
NCBI ID   WP_153714430.1    Uniprot ID   A0A5Q2QG11
Organism   Litorivicinus lipolyticus strain IMCC 1097     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2009440..2019904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH975_RS10215 (GH975_10215) - 2009468..2009656 (+) 189 WP_153714424.1 hypothetical protein -
  GH975_RS10220 (GH975_10220) - 2009653..2010330 (+) 678 WP_153714425.1 type 1 glutamine amidotransferase -
  GH975_RS10225 (GH975_10225) - 2010327..2011295 (+) 969 WP_153714426.1 asparaginase -
  GH975_RS10230 (GH975_10230) - 2011282..2012544 (-) 1263 WP_153714427.1 hypothetical protein -
  GH975_RS10235 (GH975_10235) - 2012578..2013354 (+) 777 WP_153714428.1 YdcF family protein -
  GH975_RS10240 (GH975_10240) - 2013439..2014386 (+) 948 WP_153714429.1 diguanylate cyclase domain-containing protein -
  GH975_RS12275 (GH975_10245) pilA 2014440..2014904 (-) 465 WP_153714430.1 pilin Machinery gene
  GH975_RS10250 (GH975_10250) pilA2 2015124..2015522 (-) 399 WP_153714431.1 pilin Machinery gene
  GH975_RS10255 (GH975_10255) pilB 2015727..2017424 (+) 1698 WP_153714432.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GH975_RS10260 (GH975_10260) pilC 2017437..2018681 (+) 1245 WP_153714433.1 type II secretion system F family protein Machinery gene
  GH975_RS10265 (GH975_10265) pilD 2018706..2019593 (+) 888 WP_153714434.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15472.37 Da        Isoelectric Point: 5.7273

>NTDB_id=398929 GH975_RS12275 WP_153714430.1 2014440..2014904(-) (pilA) [Litorivicinus lipolyticus strain IMCC 1097]
MRTRTQTRAQQGFTLIELMIVVAIIGILAAVAIPAYSDYTTRAKVTEGVGIAGTIKAVVSENIIAGASSNLDAGWNSGSF
SATDNVSAVAVDGATGGITVTYTAAAGGGTMIFAPFQADGSAALSFASGAHSGNFAWDCTGGTLSANFRPANCR

Nucleotide


Download         Length: 465 bp        

>NTDB_id=398929 GH975_RS12275 WP_153714430.1 2014440..2014904(-) (pilA) [Litorivicinus lipolyticus strain IMCC 1097]
ATGCGTACACGCACTCAAACACGTGCCCAGCAGGGCTTTACCCTTATCGAACTGATGATCGTGGTGGCGATTATTGGCAT
ATTAGCGGCGGTGGCGATCCCTGCGTATTCCGATTACACGACGCGGGCCAAGGTCACAGAGGGCGTCGGCATCGCCGGCA
CCATTAAAGCGGTGGTCTCGGAAAACATTATTGCCGGCGCTTCATCCAACTTGGATGCGGGCTGGAACTCAGGGTCGTTT
TCAGCCACGGACAATGTGTCGGCGGTGGCCGTCGATGGCGCGACCGGCGGTATTACCGTGACCTACACGGCGGCGGCGGG
CGGCGGCACCATGATTTTTGCACCGTTCCAGGCTGACGGTTCCGCGGCGCTGTCGTTTGCATCCGGTGCCCACAGCGGTA
ACTTTGCCTGGGACTGCACCGGCGGCACTTTGAGCGCCAACTTCCGACCGGCGAATTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-154)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q2QG11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.091

100

0.526
  pilA2 Legionella pneumophila strain ERS1305867

55.102

95.455

0.526
  pilA2 Legionella pneumophila str. Paris

54.422

95.455

0.519
  comP Acinetobacter baylyi ADP1

44.295

96.753

0.429

Multiple sequence alignment