Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GH975_RS10255 Genome accession   NZ_CP045871
Coordinates   2015727..2017424 (+) Length   565 a.a.
NCBI ID   WP_153714432.1    Uniprot ID   A0A5Q2Q8D8
Organism   Litorivicinus lipolyticus strain IMCC 1097     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2010727..2022424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH975_RS10230 (GH975_10230) - 2011282..2012544 (-) 1263 WP_153714427.1 hypothetical protein -
  GH975_RS10235 (GH975_10235) - 2012578..2013354 (+) 777 WP_153714428.1 YdcF family protein -
  GH975_RS10240 (GH975_10240) - 2013439..2014386 (+) 948 WP_153714429.1 diguanylate cyclase domain-containing protein -
  GH975_RS12275 (GH975_10245) pilA 2014440..2014904 (-) 465 WP_153714430.1 pilin Machinery gene
  GH975_RS10250 (GH975_10250) pilA2 2015124..2015522 (-) 399 WP_153714431.1 pilin Machinery gene
  GH975_RS10255 (GH975_10255) pilB 2015727..2017424 (+) 1698 WP_153714432.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GH975_RS10260 (GH975_10260) pilC 2017437..2018681 (+) 1245 WP_153714433.1 type II secretion system F family protein Machinery gene
  GH975_RS10265 (GH975_10265) pilD 2018706..2019593 (+) 888 WP_153714434.1 A24 family peptidase Machinery gene
  GH975_RS10270 (GH975_10270) coaE 2019590..2020189 (+) 600 WP_153714435.1 dephospho-CoA kinase -
  GH975_RS10275 (GH975_10275) - 2020186..2020395 (+) 210 WP_153714436.1 DNA gyrase inhibitor YacG -
  GH975_RS10280 (GH975_10280) mutT 2020359..2020781 (-) 423 WP_153714437.1 8-oxo-dGTP diphosphatase MutT -
  GH975_RS10285 (GH975_10285) argJ 2020762..2021934 (-) 1173 WP_153714438.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61537.35 Da        Isoelectric Point: 4.9780

>NTDB_id=398925 GH975_RS10255 WP_153714432.1 2015727..2017424(+) (pilB) [Litorivicinus lipolyticus strain IMCC 1097]
MGQVVLQGLARQLVSNAQISEEVAMQATADAHKAGEPFAHHVVAQSLVKSADLALTASQLYGTPLLDLNAFDLTRIPSDK
LDRKIVIEARALPLFIRGKRLYVGLSDPTDLRALDAMQFASGLTTEAIVVEADKLAAAIENYGESSGSLGDLNDDGLDNI
DLEDDSDTQTEDDPSDPGNDQPIVRFVNKMLLDAIRLGASDIHFEPYEKAYRIRYRVDGVLSEVTKPPVNMATRLAARIK
VMSKMDISERRVPQDGRIKMKLSANKAIDFRVNTLPTLWGEKIVLRILDPSSAQLGIDALGYEPDQKDMYMKALAQPQGM
ILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQTQVNTKVGLTFAEALRAFLRQDPDVVMVGEIRDLE
TAEIAIKASQTGHLVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCSHCKSAVELPPEALESEGLAPA
DIATHTLYEPVGCEHCMNGYKGRVGVYEVVRVTPAISEIIMREGNALELAKQCHLEGFKSLRQSGLQKVLQGVTSLQEIN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=398925 GH975_RS10255 WP_153714432.1 2015727..2017424(+) (pilB) [Litorivicinus lipolyticus strain IMCC 1097]
GTGGGACAGGTTGTACTCCAAGGGTTAGCGCGCCAACTGGTGTCGAACGCTCAAATCAGCGAAGAAGTCGCAATGCAGGC
GACCGCGGACGCACACAAGGCCGGCGAGCCCTTTGCGCACCATGTGGTGGCGCAATCGCTGGTCAAATCGGCCGATCTCG
CGCTGACCGCTTCGCAGCTGTACGGCACCCCGCTGCTGGATCTGAACGCCTTTGATTTAACCCGCATTCCGAGCGACAAA
CTGGATCGGAAAATAGTTATCGAGGCCCGCGCCCTGCCACTGTTCATCCGCGGCAAGCGGCTGTACGTCGGCCTCAGTGA
CCCGACCGACTTGCGCGCCTTGGATGCGATGCAATTTGCCTCCGGATTGACCACCGAAGCGATTGTGGTCGAGGCCGACA
AATTGGCTGCGGCTATCGAGAACTACGGCGAATCCAGCGGTAGCCTGGGAGATCTGAATGACGACGGTTTGGACAATATT
GACCTGGAAGACGACAGCGACACCCAAACCGAGGACGATCCGAGCGACCCCGGCAACGACCAGCCCATTGTCCGCTTCGT
CAATAAGATGTTGCTCGACGCGATTCGTCTGGGTGCGTCGGACATCCACTTCGAACCCTACGAAAAAGCCTACCGCATCC
GTTACCGGGTCGACGGGGTGCTGAGTGAAGTGACCAAACCGCCGGTCAACATGGCAACCCGTTTAGCAGCGCGCATTAAA
GTGATGTCGAAAATGGACATCTCGGAGCGCCGCGTGCCCCAGGACGGGCGCATTAAGATGAAACTGTCGGCCAACAAAGC
GATCGATTTTCGCGTCAACACCCTGCCAACTTTGTGGGGTGAGAAAATCGTGCTGCGTATTTTGGACCCCAGCAGCGCCC
AACTCGGTATCGATGCGCTGGGTTACGAGCCCGACCAAAAAGACATGTACATGAAAGCGCTGGCCCAACCCCAGGGCATG
ATTTTGGTGACCGGCCCAACCGGTTCCGGCAAAACCGTGTCGCTGTACACCGGCCTGAATATTTTAAACACCCCGGAAGT
GAACATTTCGACCGCCGAAGACCCGGTCGAAATCAACTTGGAAGGTATCAACCAAACCCAGGTCAACACCAAGGTCGGGC
TGACCTTTGCCGAAGCCCTGCGGGCGTTTTTGCGCCAAGACCCGGACGTGGTCATGGTCGGCGAGATCCGTGATTTGGAA
ACCGCTGAAATTGCGATCAAGGCCTCACAAACCGGTCACCTGGTGCTGTCGACGCTGCACACCAATTCAGCCCCGGAGAC
CCTGACTCGCTTGCGCAATATGGGGGTGCCGGCTTTCAACATCGCCACCAGCGTTTCGCTAATTATTGCCCAGCGCTTGG
CGCGGCGATTATGCAGTCACTGTAAATCCGCGGTCGAGCTGCCTCCCGAGGCGCTCGAAAGCGAGGGCCTCGCGCCCGCG
GACATCGCCACGCACACGCTTTACGAACCGGTCGGGTGCGAGCACTGCATGAACGGCTACAAGGGCCGCGTCGGTGTCTA
CGAAGTGGTCCGGGTCACTCCGGCGATATCCGAAATCATCATGCGCGAGGGCAACGCGTTGGAACTGGCCAAACAGTGCC
ACCTTGAAGGGTTTAAAAGCCTTCGCCAGTCAGGCCTGCAAAAAGTCCTGCAGGGCGTGACCAGCCTTCAAGAAATTAAC
CGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q2Q8D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.574

99.823

0.595
  pilB Acinetobacter baylyi ADP1

59.043

99.823

0.589
  pilB Legionella pneumophila strain ERS1305867

53.805

100

0.538
  pilB Vibrio cholerae strain A1552

54.286

92.92

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

52.562

93.274

0.49
  pilB Vibrio campbellii strain DS40M4

52.363

93.628

0.49
  pilF Neisseria gonorrhoeae MS11

48.754

99.469

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

41.8

88.496

0.37

Multiple sequence alignment