Detailed information NTG0398927

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GH975_RS10265
• Genome accession: NZ_CP045871
• Coordinates: 2018706..2019593 (+)
• Length: 295 a.a.
• NCBI ID: WP_153714434.1
• Uniprot ID: A0A5Q2QEY5
• Organism: Litorivicinus lipolyticus strain IMCC 1097
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2013706..2024593

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GH975_RS12275 (GH975_10245)	pilA	2014440..2014904 (-)	465	WP_153714430.1	pilin	Machinery gene
GH975_RS10250 (GH975_10250)	pilA2	2015124..2015522 (-)	399	WP_153714431.1	pilin	Machinery gene
GH975_RS10255 (GH975_10255)	pilB	2015727..2017424 (+)	1698	WP_153714432.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GH975_RS10260 (GH975_10260)	pilC	2017437..2018681 (+)	1245	WP_153714433.1	type II secretion system F family protein	Machinery gene
GH975_RS10265 (GH975_10265)	pilD	2018706..2019593 (+)	888	WP_153714434.1	A24 family peptidase	Machinery gene
GH975_RS10270 (GH975_10270)	coaE	2019590..2020189 (+)	600	WP_153714435.1	dephospho-CoA kinase	-
GH975_RS10275 (GH975_10275)	-	2020186..2020395 (+)	210	WP_153714436.1	DNA gyrase inhibitor YacG	-
GH975_RS10280 (GH975_10280)	mutT	2020359..2020781 (-)	423	WP_153714437.1	8-oxo-dGTP diphosphatase MutT	-
GH975_RS10285 (GH975_10285)	argJ	2020762..2021934 (-)	1173	WP_153714438.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 295 a.a.
• Molecular weight: 31495.43 Da
• Isoelectric Point: 6.8368

>NTDB_id=398927 GH975_RS10265 WP_153714434.1 2018706..2019593(+) (pilD) [Litorivicinus lipolyticus strain IMCC 1097]
MIQTLADAGLFLPLVTVLGLLIGSFLNVVVHRLPIMMENELRLDCEWLSAKDNGLPEPDPTAPTGPIFNLSKPRSRCPQC
DTQIRARDNIPVLSWVLLKGRCRGCDHPISARYPIVEASTAALSLLVAWVMEPSLATIAALALVWTLWALTLIDADTQLL
PDQLTLPLTWAGLLVATTGVWPVSLTDALFGAVFGYLSLWSVFHLFRILTGKHGMGAGDFKLLAALGAWMGWQVLPLIIV
LSSLVGAVVGISAIALAKMGARAPMAFGPYLAGAGLIALLWGAQLIDGYKAVAGL

Nucleotide

• Length: 888 bp

>NTDB_id=398927 GH975_RS10265 WP_153714434.1 2018706..2019593(+) (pilD) [Litorivicinus lipolyticus strain IMCC 1097]
TTGATTCAAACATTGGCCGATGCTGGCCTCTTTTTACCCCTGGTCACCGTATTGGGCCTATTAATCGGCAGCTTTCTGAA
TGTCGTCGTGCATCGACTGCCGATCATGATGGAAAACGAGTTGCGGCTGGACTGTGAGTGGCTCAGCGCAAAAGATAACG
GCCTGCCCGAACCGGACCCGACCGCGCCCACCGGTCCGATCTTTAACCTATCGAAGCCGCGCTCGCGCTGCCCACAATGC
GACACTCAAATTCGCGCGCGCGATAACATCCCGGTGTTGTCGTGGGTGCTATTAAAAGGCCGTTGCCGGGGCTGCGATCA
TCCGATCAGTGCGCGCTACCCGATCGTCGAAGCCAGCACCGCAGCGCTGTCGCTGCTGGTGGCCTGGGTGATGGAGCCCA
GCCTTGCCACCATCGCCGCACTGGCACTGGTATGGACGCTGTGGGCACTGACGCTGATTGATGCGGACACCCAATTGCTA
CCCGATCAATTAACCTTACCGCTGACCTGGGCGGGATTACTGGTCGCCACCACCGGGGTGTGGCCGGTCAGCCTGACCGA
CGCCCTGTTTGGCGCGGTCTTTGGGTACCTCAGTCTGTGGTCCGTGTTTCATTTATTTCGCATCCTAACTGGCAAGCACG
GCATGGGCGCGGGTGACTTCAAGCTGTTGGCCGCACTTGGCGCCTGGATGGGCTGGCAGGTGCTGCCGCTGATCATTGTG
CTGTCCAGTTTGGTCGGCGCCGTAGTCGGCATTTCGGCGATTGCCTTGGCCAAAATGGGCGCGCGTGCGCCAATGGCGTT
TGGGCCCTACTTGGCCGGTGCGGGTTTGATCGCCCTGTTGTGGGGCGCACAGTTAATTGACGGTTATAAGGCGGTGGCAG
GCTTATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-130)

• Type IV leader peptidase family (Peptidase_A24) (142-251)

3D structure

• AlphaFold DB: A0A5Q2QEY5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.571	94.915	0.508
pilD	Vibrio campbellii strain DS40M4	52.5	94.915	0.498
pilD	Acinetobacter baumannii D1279779	48	93.22	0.447
pilD	Acinetobacter nosocomialis M2	48	93.22	0.447
pilD	Neisseria gonorrhoeae MS11	47.368	90.169	0.427