Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ECE110019_RS01505 Genome accession   NZ_CP035751
Coordinates   322418..322858 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli E110019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 317418..327858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECE110019_RS01485 coaE 317908..318528 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  ECE110019_RS27065 - 318553..318597 (+) 45 WP_120795372.1 protein YacM -
  ECE110019_RS01490 guaC 318753..319796 (+) 1044 WP_001217338.1 GMP reductase -
  ECE110019_RS01495 hofC 319831..321033 (-) 1203 WP_000157234.1 protein transport protein HofC -
  ECE110019_RS01500 pilB 321023..322408 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  ECE110019_RS01505 pilA 322418..322858 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  ECE110019_RS01510 nadC 323061..323954 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ECE110019_RS01515 ampD 324042..324593 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ECE110019_RS01520 ampE 324590..325444 (+) 855 WP_000172028.1 beta-lactamase regulator AmpE -
  ECE110019_RS01525 aroP 325487..326860 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=304557 ECE110019_RS01505 WP_000360895.1 322418..322858(-) (pilA) [Escherichia coli E110019]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=304557 ECE110019_RS01505 WP_000360895.1 322418..322858(-) (pilA) [Escherichia coli E110019]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment