Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B2G84_RS00820 Genome accession   NZ_CP019979
Coordinates   119355..120719 (+) Length   454 a.a.
NCBI ID   WP_001085195.1    Uniprot ID   Q8E287
Organism   Streptococcus agalactiae strain Sag158     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 114355..125719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2G84_RS00795 (B2G84_00795) rpoE 115065..115640 (+) 576 WP_000418426.1 DNA-directed RNA polymerase subunit delta -
  B2G84_RS00800 (B2G84_00800) - 115913..117517 (+) 1605 WP_000170435.1 CTP synthase -
  B2G84_RS00805 (B2G84_00805) - 117626..118552 (+) 927 WP_000723859.1 alpha/beta hydrolase -
  B2G84_RS00815 (B2G84_00815) - 118747..119193 (+) 447 WP_000049186.1 dUTP diphosphatase -
  B2G84_RS00820 (B2G84_00820) radA 119355..120719 (+) 1365 WP_001085195.1 DNA repair protein RadA Machinery gene
  B2G84_RS00825 (B2G84_00825) - 120855..121352 (+) 498 WP_000214742.1 beta-class carbonic anhydrase -
  B2G84_RS00830 (B2G84_00830) - 121506..122825 (+) 1320 WP_000759706.1 FAD-containing oxidoreductase -
  B2G84_RS00835 (B2G84_00835) gltX 123034..124488 (+) 1455 WP_001284500.1 glutamate--tRNA ligase -
  B2G84_RS00840 (B2G84_00840) - 124528..125496 (-) 969 WP_000668256.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49562.84 Da        Isoelectric Point: 6.5148

>NTDB_id=182838 B2G84_RS00820 WP_001085195.1 119355..120719(+) (radA) [Streptococcus agalactiae strain Sag158]
MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKNARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGEIGLTGEIR
RVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKAVFST

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=182838 B2G84_RS00820 WP_001085195.1 119355..120719(+) (radA) [Streptococcus agalactiae strain Sag158]
GTGGCTAAGAAAAAATCAGTCTTCACCTGTCAAGAGTGTGGTTACCAATCACCTAAGTATTTGGGACGTTGTCCAAATTG
CTCAGCCTGGTCTTCATTTGTAGAAGAGGTTGAAGTTCAAGAAGTTAAAAATGCACGAGTTAGTTTGAACGGTGAAAAGT
CCCGACCGACGAAGTTAAAAGATGTTTCCTCAATTAACTATTCTAGAACTAAGACTGATATGGATGAATTTAACCGTGTT
CTAGGAGGCGGTGTTGTACCAGGAAGTTTGGTTCTGATTGGAGGAGACCCTGGGATAGGTAAATCTACTTTATTACTACA
AGTATCAACTCAGCTAGCTAATAAAGGTACAGTATTATATGTCTCAGGTGAGGAATCTGCAGAACAGATTAAGTTACGAA
GTGAGCGTCTAGGCGATATCGATAATGAATTTTACCTTTATGCCGAGACTAATATGCAAAGTATTCGGTCGGAGATTGAG
AAGATTAAACCAGATTTTCTTATTATTGATTCTATACAAACTATTATGAGTCCCGAAGTATCAAGTGTCCAAGGCTCTGT
TAGCCAAGTTCGCGAAGTGACCGCTGAATTGATGCAGTTAGCTAAAACTAATAATATTGCTACTTTTATTGTAGGTCATG
TGACTAAGGAGGGAACATTAGCAGGCCCTCGAATGCTAGAACATATGGTGGATACGGTTCTTTATTTTGAAGGGGAGCGT
CATCACACGTTCCGTATTTTACGGGCAGTCAAAAACCGTTTTGGTTCGACCAATGAAATAGGTATCTTTGAAATGCAGTC
GGGTGGTCTAGTTGAAGTTTTGAATCCAAGTCAAGTTTTTCTTGAAGAACGTTTAGACGGTGCAACAGGATCTGCTATTG
TTGTTACTATGGAAGGAACAAGGCCTATTTTAGCAGAGGTTCAAGCTTTAGTGACTCCGACTGTTTTTGGAAATGCTAAG
CGTACAACAACAGGTCTTGACTTTAATCGTGTCAGTCTTATTATGGCAGTTCTAGAAAAACGTTGTGGTTTATTGCTTCA
AAATCAAGATGCCTATTTAAAATCAGCGGGTGGTGTTAAATTGGATGAACCAGCAATTGATTTAGCTGTTGCTGTAGCTA
TTGCTTCCAGTTACAAAGAAAAACCCACAAATCCACAAGAATCATTTATTGGAGAAATTGGACTCACTGGTGAGATTCGT
CGCGTGACACGTATTGAGCAACGTATTAATGAGGCTAGTAAATTGGGCTTTACTAAAATTTACGCACCAAAGAATTCTCT
AGCTGGGATTGAAATACCTAAAGGCATTGATGTTATTGGTGTGACAACTGTTAGTCAGGTCTTAAAAGCAGTGTTTTCAA
CATAA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E287

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.079

99.78

0.879
  radA Streptococcus pneumoniae D39

88.079

99.78

0.879
  radA Streptococcus pneumoniae R6

88.079

99.78

0.879
  radA Streptococcus pneumoniae TIGR4

88.079

99.78

0.879
  radA Streptococcus mitis NCTC 12261

88.079

99.78

0.879
  radA Streptococcus mitis SK321

87.859

99.78

0.877
  radA/sms Bacillus subtilis subsp. subtilis str. 168

62.914

99.78

0.628

Multiple sequence alignment