Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   B2G84_RS00415 Genome accession   NZ_CP019979
Coordinates   65225..66223 (+) Length   332 a.a.
NCBI ID   WP_000196634.1    Uniprot ID   Q8E2D9
Organism   Streptococcus agalactiae strain Sag158     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 60225..71223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2G84_RS00400 (B2G84_00400) - 61151..62542 (+) 1392 WP_001079802.1 hypothetical protein -
  B2G84_RS00405 (B2G84_00405) purB 62567..63865 (+) 1299 WP_000572887.1 adenylosuccinate lyase -
  B2G84_RS00410 (B2G84_00410) comR 64017..64928 (+) 912 WP_000912100.1 helix-turn-helix domain-containing protein Regulator
  B2G84_RS00415 (B2G84_00415) ruvB 65225..66223 (+) 999 WP_000196634.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  B2G84_RS00420 (B2G84_00420) - 66375..66812 (+) 438 WP_000754813.1 low molecular weight protein-tyrosine-phosphatase -
  B2G84_RS00425 (B2G84_00425) - 66819..67199 (+) 381 WP_000787700.1 membrane protein -
  B2G84_RS00430 (B2G84_00430) - 67196..68974 (+) 1779 WP_001220909.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37557.94 Da        Isoelectric Point: 4.5391

>NTDB_id=182836 B2G84_RS00415 WP_000196634.1 65225..66223(+) (ruvB) [Streptococcus agalactiae strain Sag158]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATVK
AYEHLGYQRFDK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=182836 B2G84_RS00415 WP_000196634.1 65225..66223(+) (ruvB) [Streptococcus agalactiae strain Sag158]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCCGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTACTTTATAGTGCAATGGAAGACTTCTATATTGACA
TTATGATCGGTGCAGGAGAAACTAGTAGAAGTGTTCATCTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAATGATTTGAC
AGAAATTATTGAGCGTACAGCAGACATTTTTGAAATGAAAATTACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGTTAAG
GCATATGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)

RuvB AAA lid domain (AAA_lid_4)

(181-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E2D9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae TIGR4

90.06

100

0.901
  ruvB Streptococcus pneumoniae R6

89.759

100

0.898
  ruvB Streptococcus pneumoniae D39

89.759

100

0.898
  ruvB Bacillus subtilis subsp. subtilis str. 168

59.819

99.699

0.596
  ruvB Helicobacter pylori 26695

53.074

93.072

0.494
  ruvB Synechocystis sp. PCC 6803

50.479

94.277

0.476

Multiple sequence alignment