Detailed information    

experimental Experimentally validated

Overview

Name   ruvB   Type   Machinery gene
Locus tag   SP_RS01270 Genome accession   NC_003028
Coordinates   231600..232598 (+) Length   332 a.a.
NCBI ID   WP_001808442.1    Uniprot ID   Q97SR6
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

The RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex.


Genomic Context

Location: 226600..237598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS01240 (SP_0255) - 227448..228143 (+) 696 WP_001144899.1 GNAT family N-acetyltransferase -
  SP_RS01245 (SP_0256) - 228155..228571 (+) 417 WP_000628406.1 GNAT family N-acetyltransferase -
  SP_RS12620 (SP_0257) ltrA 229365..230621 (+) 1257 Protein_238 group II intron reverse transcriptase/maturase -
  SP_RS01265 (SP_0258) - 231373..231465 (-) 93 WP_000970381.1 type I toxin-antitoxin system Fst family toxin -
  SP_RS01270 (SP_0259) ruvB 231600..232598 (+) 999 WP_001808442.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  SP_RS01275 (SP_0260) - 232579..233151 (+) 573 WP_001098903.1 nucleotidyltransferase family protein -
  SP_RS01280 (SP_0261) - 233382..234140 (+) 759 WP_000466719.1 isoprenyl transferase -
  SP_RS01285 (SP_0262) - 234149..234952 (+) 804 WP_000189859.1 phosphatidate cytidylyltransferase -
  SP_RS01290 (SP_0263) eeP 234974..236233 (+) 1260 WP_000900658.1 RIP metalloprotease RseP Regulator

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 37278.70 Da        Isoelectric Point: 4.5018

>NTDB_id=684 SP_RS01270 WP_001808442.1 231600..232598(+) (ruvB) [Streptococcus pneumoniae TIGR4]
MSRILDNEMMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNDLEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=684 SP_RS01270 WP_001808442.1 231600..232598(+) (ruvB) [Streptococcus pneumoniae TIGR4]
ATGAGTAGAATTTTAGATAATGAGATGATGGGTGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATTGGTCAGGATAAGGTTAAGGATCAACTTCAGATCTTTATCGAAGCCGCTAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGCCCTCCAGGGCTTGGGAAAACAACCATGGCTTTTGTCATTGCCAATGAACTGGGAGTCAATCTC
AAGCAAACGTCTGGTCCTGTTATCGAAAAAGCGGGTGATTTGGTAGCGATTTTGAATGATTTAGAGCCTGGAGATGTCCT
CTTTATTGACGAGATTCATCGCTTACCCATGTCAGTGGAAGAAGTGCTTTATAGTGCCATGGAGGACTTCTACATCGATA
TTATGATTGGGGCTGGTGAAGGCAGTCGCAGTGTTCATTTGGAGTTGCCTCCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCAAATCCACTACGGGCACGTTTTGGGATTACAGGTCATATGGAGTATTATGCTCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACCCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGAACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTTATCGAT
GATGTTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
CACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTGAATATCGCCGAAGAGCGTGAGACAG
TTGAAGACATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q97SR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

99.096

100

0.991
  ruvB Streptococcus pneumoniae D39

99.096

100

0.991
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.429

98.193

0.593
  ruvB Helicobacter pylori 26695

50.774

97.289

0.494
  ruvB Synechocystis sp. PCC 6803

49.84

94.277

0.47

References

[1] H Tettelin et al. (2001) Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science (New York, N.Y.) 293(5529):498-506. [PMID: 11463916]