Detailed information    

experimental Experimentally validated

Overview

Name   ruvB   Type   Unclear
Locus tag   SGL_RS14255 Genome accession   NC_000911
Coordinates   2651866..2652951 (-) Length   361 a.a.
NCBI ID   WP_010873577.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   require for natural transformation   
Unclear

Function

In every case, the efficiency of transformation to Kmr phenotype was approximately threefold lower in the ruvB - mutant than in the WT strain.


Genomic Context

Location: 2646866..2657951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS14240 (sll0616) secA 2647386..2650184 (-) 2799 WP_010873574.1 preprotein translocase subunit SecA -
  SGL_RS14245 (sll0615) - 2650377..2650997 (-) 621 WP_010873575.1 TMEM165/GDT1 family protein -
  SGL_RS14250 (sll0614) - 2651440..2651838 (-) 399 WP_010873576.1 DUF4253 domain-containing protein -
  SGL_RS14255 (sll0613) ruvB 2651866..2652951 (-) 1086 WP_010873577.1 Holliday junction branch migration DNA helicase RuvB Unclear
  SGL_RS19980 thiO 2653153..2654124 (+) 972 Protein_2489 glycine oxidase ThiO -
  SGL_RS19985 - 2654302..2655123 (+) 822 WP_369791791.1 thiazole synthase -
  SGL_RS14265 (slr0634) - 2655459..2656037 (+) 579 WP_010873579.1 DUF3782 domain-containing protein -
  SGL_RS14270 (slr0635) - 2656139..2657077 (+) 939 WP_010873580.1 YgfZ/GcvT domain-containing protein -
  SGL_RS14275 (sll0611) - 2657148..2657594 (-) 447 WP_010873581.1 hypothetical protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40053.15 Da        Isoelectric Point: 6.8865

>NTDB_id=1421 SGL_RS14255 WP_010873577.1 2651866..2652951(-) (ruvB) [Synechocystis sp. PCC 6803]
MAIKRSGNNNLSPNVKSDLLSPEVIPQERSTSPELEQQEASLRPQRLADYIGQRDLKEVLRIAIQAAQGRQEAIDHLLLY
GPPGLGKTTLALILAEEMQVRCKITAAPALERPRDITGLLLALQPGDILFIDEIHRLNRLTEELLYPAMEDFRLDITMGK
GQSAKVRSLKLAHFTLVGATTKVGSLTSPLRDRFGLIQRLRFYEVDELQQIILRTAGILSVSISPTGAEAIAMRARGTPR
IANRLLKRVRDYAQVKQQPEIDPALASEALDLYQVDKRGLDWTDRLVLQTLIEQFQGGPTGLEAIAAATGEDAKTIEEVY
EPYLLQIGYLARTSRGRIATTAAYEHLGLTPPTPLLPWKES

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=1421 SGL_RS14255 WP_010873577.1 2651866..2652951(-) (ruvB) [Synechocystis sp. PCC 6803]
ATGGCCATTAAACGTTCCGGCAACAATAATCTTTCTCCCAACGTTAAATCCGATCTCCTTTCCCCGGAAGTTATTCCCCA
GGAACGCAGTACCAGCCCAGAGTTAGAACAGCAAGAGGCATCCCTCAGGCCCCAACGGTTAGCGGATTACATTGGCCAAC
GGGATCTCAAGGAAGTGTTGCGCATTGCTATCCAAGCGGCCCAAGGTAGGCAAGAGGCGATCGACCATCTGCTGTTATAC
GGCCCACCGGGGTTGGGCAAAACCACCCTAGCTCTAATTTTGGCGGAGGAAATGCAGGTACGTTGCAAAATTACTGCGGC
TCCGGCGTTAGAACGTCCCAGGGATATTACGGGGCTACTATTGGCTCTGCAACCGGGGGATATTTTGTTTATCGACGAAA
TTCACCGTTTAAATCGCCTAACCGAGGAATTGCTCTACCCTGCCATGGAGGATTTTCGTTTGGACATCACTATGGGCAAA
GGCCAAAGCGCCAAAGTCCGCAGTTTAAAGTTAGCCCACTTTACTCTTGTGGGGGCTACCACCAAAGTAGGTTCTTTGAC
TTCGCCGCTGCGGGATCGGTTTGGCTTAATTCAAAGATTGAGATTTTACGAGGTTGATGAGTTGCAACAAATTATCCTCC
GCACCGCTGGTATTTTATCCGTATCTATTTCCCCCACAGGGGCCGAGGCGATCGCCATGAGAGCCAGGGGAACTCCCCGC
ATTGCCAACCGTTTACTGAAAAGGGTGCGGGATTACGCCCAGGTTAAACAACAGCCGGAAATTGACCCTGCCCTTGCGTC
GGAAGCATTGGATTTATACCAAGTGGATAAACGGGGTTTGGATTGGACAGACCGCTTAGTTTTACAAACTTTAATTGAAC
AATTCCAGGGAGGCCCCACCGGCCTAGAGGCGATCGCCGCTGCCACAGGGGAAGATGCCAAAACCATTGAGGAAGTGTAC
GAACCCTACCTGCTACAAATTGGTTACCTGGCCCGCACCAGTCGAGGCCGCATTGCCACCACCGCCGCCTATGAACACCT
AGGGTTGACTCCCCCCACTCCCCTGCTACCTTGGAAGGAAAGTTAA

Domains

Predicted by InterproScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(42-200)

RuvB AAA lid domain (AAA_lid_4)

(203-276)

RuvB C-terminal winged helix domain (RuvB_C)

(278-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Francis Domain et al. (2004) Function and regulation of the cyanobacterial genes lexA, recA and ruvB: LexA is critical to the survival of cells facing inorganic carbon starvation. Molecular Microbiology 53(1):65-80. [PMID: 15225304]